[PDF] Top 20 Development of PCR Assays for Species and Type Specific Identification of Pasteurella multocidaIsolates
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Development of PCR Assays for Species and Type Specific Identification of Pasteurella multocidaIsolates
... identify type B ...from type E:2 strains that cause HS in ...identify type B strains of other somatic serotypes that have been implicated in septicemic pasteurello- sis of wild ...the PCR ... See full document
5
Molecular diagnosis of cutaneous leishmaniasis and identification of the causative Leishmania species in Morocco by using three PCR based assays
... Leishmania species to- gether with the clinical presentation of the resulting disease are frequently used for species ...for species identification [16]. Thus, the diversity of the ... See full document
9
Quantitative Real Time PCR Assay Panel for Detection and Type Specific Identification of Epidemic Respiratory Human Adenoviruses
... 35), species- and type-specific PCR assays are capable of revealing coinfections with different HAdVs that otherwise might go ...qPCR assays that contained two HAdV types, one ... See full document
5
Species Specific Identification of Human Adenoviruses by a Multiplex PCR Assay
... multiplex PCR assay that could supplant hemagglutination for Ad species ...Whereas PCR assays described for identification of Ad species and serotypes have targeted the hexon ... See full document
8
Species specific and ubiquitous DNA based assays for rapid identification of Staphylococcus epidermidis
... on PCR assays performed directly from bacterial colonies or from a standardized ...this PCR protocol includes only 30 cycles, our results show that the sensitivity levels achieved ...midis ... See full document
6
Evaluation of 11 PCR Assays for Species Level Identification of Campylobacter jejuni and Campylobacter coli
... 11 PCR assays described for the species identification of Campylobacter jejuni and Campylobacter coli by using 111 type, reference, and field strains of ...six assays, an ... See full document
7
Development of a PCR Assay for Identification of Staphylococci at Genus and Species Levels
... API identification systems (bioMe´rieux Vitek) (1, 21) and automated systems such as the MicroScan system (Dade Behring ...of PCR-based methods for the species-specific detection of ... See full document
7
Species Identification and Subtyping ofUreaplasma parvum and Ureaplasma urealyticumUsing PCR Based Assays
... Bacterial strains. Two sets of reference strains of all 14 serovars of U. urea- lyticum (including biovars 1 and 2, referred to hereafter as U. parvum and U. urealyticum, respectively) were used as previously described ... See full document
5
Development of multiplex real time PCR assays for identification of members of the Anopheles funestus species group
... target species of the An. funestus group from the melting temperature of the PCR amplicons which ranged in size from 146 to 587 ...each species of ...each species. The detec- tion limit of ... See full document
9
The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniaeand comparison with four other species specific PCR assays
... μl PCR GoTaqGreen Mix (Promega Benelux, Leiden, the Netherlands), 2 μM of each of the forward primer spn9802-143F and the reverse primer spn9802- 304R and 1 μl of the DNA ...All PCR products were ... See full document
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Development of multiplex real-time PCR assays for identification of members of the Anopheles funestus species group
... target species of the An. funestus group from the melting temperature of the PCR amplicons which ranged in size from 146 to 587 ...each species of ...each species. The detec- tion limit of ... See full document
10
Evaluation of Multiplex Type Specific Real Time PCR Assays Using the LightCycler and Joint Biological Agent Identification and Diagnostic System Platforms for Detection and Quantitation of Adult Human Respiratory Adenoviruses
... our assays were specific to the target HAdV ...adenovirus assays with different adenovirus types within species HAdV-B (types B3, B7, B11, B14, and B21), HAdV-E4, or representatives from other ... See full document
7
Species Specific and Ubiquitous DNA Based Assays for Rapid Identification of Staphylococcus aureus
... oped specific and ubiquitous PCR assays for the detection of 16 other species and three genera of the most frequently en- countered bacterial pathogens which account for approxi- mately 85% of ... See full document
6
Development of Rapid Serotype Specific PCR Assays for Eight Serotypes of Streptococcus suis
... eight-serotype-specific PCR. To evaluate the results obtained with the PCR assays, these tonsillar specimens were subjected to an extended bacteriological examination ... See full document
6
Duplex Reverse Transcription PCR Followed by Nested PCR Assays for Detection and Identification of Brazilian Alphaviruses and Flaviviruses
... (RT-PCR) assays using species-specific primers have been de- scribed for certain alphaviruses (3, 19, 20, 25, 28, 33, 38, 39) and certain flaviviruses (7, 8, 9, 12, 13, 16, 21, 23, 27, ...no ... See full document
7
Development of Reverse Transcription PCR Assays Specific for Detection of Equine Encephalitis Viruses
... (nested PCR), respectively (Fig. 2C). WEE-specific RT-PCR and seminested ...RT-PCR–seminested PCR (Table 3, com- binations I and Isn) and a RT-PCR–nested PCR (combina- ... See full document
9
Specific Identification of Listeria welshimeri and Listeria monocytogenes by PCR Assays Targeting a Gene Encoding a Fibronectin Binding Protein
... our PCR assays are able to correctly identify other isolates of ...aim, PCR amplifi- cations with primer pairs G398-G399 and G398-G400 were performed with chromosomal DNA (100 ng) purified from 15 ... See full document
6
Identification of Streptococcus sanguinis with a PCR Generated Species Specific DNA Probe
... the PCR profiles of type strain ATCC 10556 and all other strains of ...coccus species; one was from nt 1 to 111 and the other was from nt 583 to ... See full document
6
Development of PCR based hybridization protocol for identification of streptococcal species
... to detect S. uberis RNA may be the secondary structure of the 16S rRNA molecule, which has been predicted to be a very complex mixture of hairpin and loop structures (15). The sec- ondary structure of 16S rRNA in the ... See full document
6
Development of a 16S rRNA PCR RFLP Assay for Bartonella identification: Applicability in the Identification of Species Involved in Human Infections
... gene PCR-RFLP scheme to identify all currently described Bartonella ...described species of ...different species of Bartonella, including human pathogenic ... See full document
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