[PDF] Top 20 Development of a Multiplex Bead-Based Assay for Detection of Hepatitis C Virus
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Development of a Multiplex Bead-Based Assay for Detection of Hepatitis C Virus
... improving detection by using mul- tiplex ...promising multiplex techniques uses digital signal processing to classify small polystyrene ...each bead population (13, ...the detection of their ... See full document
5
Simultaneous Detection of Multiplex Amplified Human Immunodeficiency Virus Type 1 RNA, Hepatitis C Virus RNA, and Hepatitis B Virus DNA Using a Flow Cytometer Microsphere Based Hybridization Assay
... The development of nucleic acid amplification technologies has found many applications in biomedical research and in diagnostic ...use multiplex PCR or re- verse transcription-PCR (RT-PCR) in order to ... See full document
6
Development of a melting curve based multiplex real time PCR assay for simultaneous detection of Streptococcus agalactiae and genes encoding resistance to macrolides and lincosamides
... the multiplex real-time PCR assay in clinical samples in comparison to culture based analysis The performance of real-time PCR in GBS isolates and clinical samples was compared with the results ... See full document
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The development of a GeXP based multiplex reverse transcription PCR assay for simultaneous detection of sixteen human respiratory virus types/subtypes
... different denaturation temperatures and the amplifica- tion cycle conditions were determined in the current protocol. The chimeric primers consisted of a specific primer sequence fused to the universal primer sequence. ... See full document
8
Development of an isothermoal amplification based assay for rapid visual detection of an Orf virus
... RPA-LFD assay on samples Initially, the feasibility of the ORFV RPA-LFD assay for diagnosis was tested validated with known positive samples (n = 9) and negative samples (n = 9) which was confirmed by ORFV ... See full document
6
Comparison of a Multiplex Real Time PCR Assay with a Multiplex Luminex Assay for Influenza Virus Detection
... the virus was not detected in our laboratory during the study ...fluorescent detection of the bead and hybridization signals and can detect 100 ...FLULUM assay to use magnetic beads in order ... See full document
6
Multiplex Real Time Reverse Transcription PCR Assay for Determination of Hepatitis C Virus Genotypes
... genotyping assay was described by Okamoto et al. (24). The assay utilized a first-round amplification of a large section of the core region, followed by a set of second nested amplification reactions each ... See full document
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Development of Multiplex PCR Ligase Detection Reaction Assay for Detection of West Nile Virus
... multiplexed detection of blood-borne bacterial infectious agents ...the detection of blood-borne viral pathogens, both in clinical specimens and for environmental ...a multiplex RT-PCR/LDR for ... See full document
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Multiplex malaria antigen detection by bead based assay and molecular confirmation by PCR shows no evidence of Pfhrp2 and Pfhrp3 deletion in Haiti
... Unlike many countries in the South American region, the country of Haiti does not appear to harbor Pfhrp2- or Pfhrp3-deleted parasites. Clinical cases of Pfhrp2 gene deletion were first reported in Peru’s Amazonian ... See full document
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Heminested Multiplex Reverse Transcription PCR for Detection and Differentiation of Norwalk Like Virus Genogroups 1 and 2 in Fecal Samples
... RT-PCR assay and EM. To assess the sen- sitivity level of the multiplex RT-PCR assay in relation to that of EM, 95 fecal samples that were positive for NLV by either EM or RT-PCR were chosen for ... See full document
5
Development of a Bead-Based Multiplex Immunoassay for Simultaneous Quantitative Detection of IgG Serum Antibodies against Measles, Mumps, Rubella, and Varicella-Zoster Virus
... The assessment of the antibody concentrations in the serum samples was performed essentially as previously described (19). The reference se- rum (RUBI-1-94), serum samples, and control sera were prediluted in PBS ... See full document
5
Multiplex detection in tonsillar tissue of all known human polyomaviruses
... acid detection and ...high-throughput multiplex DNA detection. An assay has earlier been set up for the detection of 10 HPyVs [52, ...further development of this multiplex ... See full document
8
Bead Based Multiplex Genotyping of Human Papillomaviruses
... (RLB) assay, is sensitive and well ...the development of multiplex human papillomavirus genotyping (MPG), a quanti- tative and sensitive high-throughput procedure for the identification of multiple ... See full document
9
Development of a multiplex fluorescence immunological assay for the simultaneous detection of antibodies against Cooperia oncophora, Dictyocaulus viviparus and Fasciola hepatica in cattle
... Luminex assay, field samples obtained from naturally infected grazing animals during a trial in Poland have been used for comparison of the new Luminex® assay with already established ...of multiplex ... See full document
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Automated Multiplex Assay System for Simultaneous Detection of Hepatitis B Virus DNA, Hepatitis C Virus RNA, and Human Immunodeficiency Virus Type 1 RNA
... antibody assay and an HIV-1 p24 antigen assay. In most cases, RNA detection preceded antigen detection, which in turn preceded antibody ...RNA detection preceded antigen ... See full document
9
Development of a fluorescent probe based recombinase polymerase amplification assay for rapid detection of Orf virus
... Orf virus (ORFV) is a prototype member of the Parapoxvirus genus within the Poxviridae family and the viral genome consists of a linear double-stranded DNA (137–139 kbp in ... See full document
7
Quantitation of hepatitis C virus genome molecules in plasma samples
... A competitive reverse transcription PCR cRT-PCR-based assay for the quantitative detection of hepatitis C virus HCV viremia was developed, optimized, and applied to the direct molecular [r] ... See full document
6
Rapid and simultaneous detection of human hepatitis B virus and hepatitis C virus antibodies based on a protein chip assay using nano gold immunological amplification and silver staining method
... parallel detection of thousands of addressable elements in a single ...visual detection based on two-probe sandwich hybridization/nanoparticle amplifi- cation have been employed, and HBV and HCV gene ... See full document
8
Highly Sensitive Multiplex Assay for Detection of Human Immunodeficiency Virus Type 1 and Hepatitis C Virus RNA
... immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV) ...a multiplex HIV-1/HCV assay for simultaneous detection of HIV-1 and HCV, and discriminatory assays for ... See full document
12
Detection of Respiratory Viruses from ARTI Patients by xTAG RVP Fast v2 Assay and Conventional Methods
... is based on a simpler protocol and has shorter turnaround times (approximately 4 h) than conventional ...only assay to use the MAGPIX system. The xTAG RVP Fast v2 assay is a qualitative ... See full document
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