[PDF] Top 20 Development of a Species Specific fur Gene Based Method for Identification of the Burkholderia cepacia Complex
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Development of a Species Specific fur Gene Based Method for Identification of the Burkholderia cepacia Complex
... The fur gene protocol has a number of advantages over those developed previously, as it does not rely on biochemical measures; it can identify strains to the species level (unlike 16S rRNA ... See full document
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Multilocus Sequence Typing Scheme That Provides Both Species and Strain Differentiation for the Burkholderia cepacia Complex
... recA gene, such as restriction fragment length polymorphisms (RFLP), are transferable but offer limited res- olution and are primarily applied as a means of identification at the BCC species level ... See full document
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Phenotypic Methods for Determining Genomovar Status of the Burkholderia cepacia Complex
... and species are shown in Table ...B. cepacia complex, except for ...B. cepacia complex isolates (excluding ...B. cepacia by the API 20 NE ...five species, of which four ... See full document
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Differentiation of Species Combined into the Burkholderia cepacia Complex and Related Taxa on the Basis of Their Fatty Acid Patterns
... the species level based on the results obtained by genotypic methods, such as restriction fragment length polymorphism analysis of the recA gene or 16S rRNA genes (17, 18) and recA ... See full document
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Novel Pan Genomic Analysis Approach in Target Selection for Multiplex PCR Identification and Detection of Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia cepacia Complex Species: a Proof of Concept Study
... six gene targets ...the identification of lineage-specific genes, and yet the computa- tional power demanded by such methods appears prohibitive; the “Benchmark Data set” of 100 proteomes used by the ... See full document
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Diversity of the parB and repA genes of the Burkholderia cepacia complex and their utility for rapid identification of Burkholderia cenocepacia
... PCR method was tested on 142 different ST ...unassigned species status (Bcc Groups K, E, Bcc2 to 6, and Bcc group 8) were included in the study making the strain collection examined one of the most broad in ... See full document
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DNA Based Diagnostic Approaches for Identification of Burkholderia cepacia Complex, Burkholderia vietnamiensis, Burkholderia multivorans,Burkholderia stabilis, and Burkholderia cepacia Genomovars I and III
... B. cepacia complex and closely related ...rRNA gene in strains representative of the ...the gene from all bacterial species tested to date ...rRNA gene (and recA [see below]) for ... See full document
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Correlation of wbiI Genotype, Serotype, and Isolate Source within Species of the Burkholderia cepacia Complex
... the Burkholderia cepacia complex (Bcc) are opportunistic pathogens that can infect the lungs of cystic fibrosis (CF) patients and can be transmitted among these patients, causing epidemics in the CF ... See full document
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Distinguishing Species of the Burkholderia cepacia Complex and Burkholderia gladioli by Automated Ribotyping
... Several species belonging to the genus Burkholderia are clinically relevant, opportunistic pathogens that inhabit major environmental ...adequate identification and epidemiological characterization ... See full document
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Taxonomy and Identification of the Burkholderia cepacia Complex
... B. cepacia-like organisms are available. The choice of what identification tools to use depends on their availability and the mission of the laboratory ...B. cepacia complex or the genus ... See full document
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SNaPBceBcon: a Practical Tool for Identification and Genotyping of Burkholderia cepacia and Burkholderia contaminans
... he Burkholderia cepacia complex (Bcc) is a very heterogeneous group of 20 bacterial species capable of adapting to an innu- merable range of environments ...Bcc identification can be ... See full document
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Detection of Burkholderia cepacia Complex in Patients with Cystic Fibrosis
... specimens. Burkholderia species colonies were visible on almost all BCSA plates after 48 ...B.cepacia complex initially appeared as pinpoint colonies which finally changed to small, gray, and ... See full document
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Six Year Molecular Analysis of Burkholderia cepacia Complex Isolates among Cystic Fibrosis Patients at a Referral Center for Lung Transplantation
... B. cepacia from referred patients to our clinic CF patient popu- lation has not ...B. cepacia complex-infected patients (patients 12 to 14, whose isolates were in cluster C, and patient 16, whose ... See full document
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Antibiotic susceptibility pattern of Burkholderia cepacia complex from various clinical samples in a tertiary care center: A one year prospective study
... Burkholderia cepacia is a cluster of at least 18 closely related genomic species (or genomovars) now called the Burkholderia cepacia complex (BCC) (Table ...The ... See full document
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Evaluation of Various Culture Media for Detection of Rapidly Growing Mycobacteria from Patients with Cystic Fibrosis
... The species and subspecies identities of all strains had been previously confirmed by sequencing of at least two of three house- keeping genes (rpoB, hsp65, and sodA), as described previously ... See full document
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Monitoring clinical and microbiological evolution of a cystic fibrosis patient over 26 years: experience of a Brazilian CF Centre
... rRNA gene in an Illumina MiSeq instrument ...Although Burkholderia was remarkably predominant, other genera related to CF lung infections were also found: Pseudo- monas ... See full document
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Implementation of a successful eradication protocol for Burkholderia Cepacia complex in cystic fibrosis patients
... was based on identifying an aggressive combin- ation of inhaled and intravenous therapies with broad coverage to obtain maximal in-vivo treatment synergy as multiple patient strains demonstrated pan-resistance ... See full document
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Method for Rapid Identification and Differentiation of the Species of the Mycobacterium chelonae Complex Based on 16S 23S rRNA Gene Internal Transcribed Spacer PCR Restriction Analysis
... SGM species (10 l each) were resolved on 1% agarose using the Trevigel gel matrix (Trevigen, Gaithersburg, MD) with 1⫻ Tris-acetate/EDTA electrophoresis buffer ...the Gene Clean II kit (Bio 101 Systems, ... See full document
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Burkholderia cepacia complex: clinical course in cystic fibrosis patients
... B. cepacia complex, the outcome varies considerably, from a rapid fatal decline in lung function and bacteremia to an acceleration of pulmonary function decline or to chronic asymptomatic infection, ... See full document
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Direct PCR Detection of Burkholderia cepacia Complex and Identification of Its Genomovars by Using Sputum as Source of DNA
... Burkholderia cepacia is a multiresistant and transmissible opportunistic pathogen that frequently impairs the clinical state of patients with cystic fibrosis (CF) (1, 3, 9, ...B. cepacia ... See full document
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