[PDF] Top 20 Developmental Commitment in Dictyostelium discoideum

... starvation, Dictyostelium discoideum cells halt cell proliferation, aggregate into multicellular organisms, form migrating slugs, and undergo morphogenesis into fruiting bodies while differentiating into ... See full document

... in Dictyostelium genomic DNA, but the recent sequencing of the Dictyostelium genome revealed a candidate DNA methyltransferase gene ...of Dictyostelium genomic DNA with antibodies raised against ... See full document

... PSF secretion ceases as soon as the food supply is exhausted and is replaced by a second starvation factor. Conditioned Medium Factor (CMF). In contrast to PSF, CMF has been purified and the gene encoding it cloned (Jain ... See full document

... D. discoideum har- bors a single Gβ, one Gγ, and twelve Gα subunits ...D. discoideum development is relatively sim- ple as compared to higher ...to developmental morphology and cellular ... See full document

... species, Dictyostelium discoideum (hereafter referred to as Dictyostelium) was discovered in 1933 by Kenneth Raper in the Craggy Mountains of Western North Carolina (Raper, ...the ... See full document

... Expression and cell-type-specific localization of Roco4. RT- PCR analysis suggested that expression of Roco4 is upregu- lated during the later stages of development, consistent with the observed phenotype of roco4-null ... See full document

... D ictyostelium normally exists as a haploid, unicellular amoeba which lives in soil and feeds upon bacteria by phagocytosis. The amoebae divide by binary fission and increase in number until their food supply is ... See full document

... Examination of the production of AprA and CfaD from 12 to 20 h poststarvation revealed an increase in the extracellular levels of both chalones in wild-type cells. This increase coin- cides with the time when the number ... See full document

... D. discoideum we found massive transcriptional changes and complex phenotypes of varying severity for ATG12‾, ATG16‾ and ATG12‾/16‾ knock-out cells implying that ATG12 and ATG16 have, in addition to their role in ... See full document

... of developmental biology of Dictyostelium (Kay et ...the developmental morphology was exam- ined with gene knockout mutants, which is an in vivo ... See full document

... The most outstanding feature of Dictyostelium protein synthesis as revealed on these gels is the finding that a single species of protein, Band B, rises from 4-6 percent of the total pro[r] ... See full document

... During Dictyostelium development cell type differentiation and gene expression patterns are tightly ...most Dictyostelium cell ...certain developmental time is real or the reflection of a previous ... See full document

... D. discoideum and in the group 2 species ...into Dictyostelium cells together with BamHI or an enzyme that produces the same sticky ...the Dictyostelium gene ...into Dictyostelium, where it ... See full document

... One of these proteins, PsA, a developmentally regulated prespore- specific surface glycoprotein, has previously been shown to exist in three size forms due to alle[r] ... See full document

... few Dictyostelium cells, rblA ⫺ cells proliferate more slowly than wild-type cells ...of Dictyostelium cells moves across a lawn of bacteria, rblA ⫺ cells can proliferate and move at roughly the same rate ... See full document

... Labeling of DNA with propidium iodide requires prior fixation of cells. Since the aim of this study was simultaneously to estimate relative DNA content (or cell cycle phases) and cytoplasmic calcium levels, we needed a ... See full document

... In animals, contests and fights are often won by the largest individuals or those in the best condition. Consistent with this, D. discoideum cells fed glucose are more likely to become spores over those starved of ... See full document

... Like the cyst walls of other protists, the spore coat of Dictyostelium discoideum is formed de novo to protect the enclosed dormant cell from stress. Spore coat assembly is initiated by exocytosis of ... See full document

... Linkage analysis in Dictyostelium discoideum using multiply marked tester strains: establishment of linkage group VI1 and the reassessment of earlier linkage data.. whi[r] ... See full document

... Classical Dictyostelium mutagenesis using DNA-damaging agents causes multiple mutations, so we presume that both AX2 and AX3 strains have several sec- ondary mutations which are responsible for this multifac- eted ... See full document