[PDF] Top 20 Diagnosis of Bartonella Endocarditis by a Real Time Nested PCR Assay Using Serum
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Diagnosis of Bartonella Endocarditis by a Real Time Nested PCR Assay Using Serum
... OSN-PCR assay that was performed in a different room. In this assay, the two primer pairs had different hybridization temper- atures and the reaction tubes were not opened during the entire ... See full document
7
Value of Microimmunofluorescence for Diagnosis and Follow-up of Bartonella Endocarditis
... the diagnosis and study of rickettsioses and collected data for patients with blood culture- negative endocarditis between January 1995 and December ...45,000 serum specimens for ... See full document
7
Detection and Measurement of Fungal Burden in a Guinea Pig Model of Invasive Pulmonary Aspergillosis by Novel Quantitative Nested Real Time PCR Compared with Galactomannan and (1,3) β d Glucan Detection
... the diagnosis and to enable diagnostic driven rather than empirical antifungal treatment ...in serum, bronchoalveolar lavage (BAL) fluid, or other body fluids, has proven to be an im- portant advance in the ... See full document
7
A nested real time PCR assay for the quantification of Plasmodium falciparum DNA extracted from dried blood spots
... Blood samples in this study were obtained during passive and active malaria surveillance visits (occurring every two weeks) in Kalifabougou from May 2011 to December 2011 as previously described [9]. For each ... See full document
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Detection of Circulating Aspergillus fumigatus DNA by Real Time PCR Assay of Large Serum Volumes Improves Early Diagnosis of Invasive Aspergillosis in High Risk Adult Patients under Hematologic Surveillance
... a PCR to detect Aspergillus DNA in whole blood or serum by weekly screening for adult patients with hematological disorders who are at high-risk for IA (5, 9, 11, 13, ...the assay, as performed, was ... See full document
6
Real-Time PCR with Serum Samples Is Indispensable for Early Diagnosis of Acute Q Fever
... diagnostic assay. Since blood cells and bacteria are removed from serum by centrifugation of blood, we speculate that we detect DNA fragments rather than intact ...burnetii real-time ... See full document
5
Novel Wide Range Quantitative Nested Real Time PCR Assay for Mycobacterium tuberculosis DNA: Clinical Application for Diagnosis of Tuberculous Meningitis
... WR-QNRT-PCR assay results in the blinded assay demonstrated sufficient reproducibility and strengthened the clinical significance of this assay (Table ...WR-QNRT-PCR assay was ... See full document
10
Comparison of Conventional, Nested, and Real Time Quantitative PCR for Diagnosis of Scrub Typhus
... assays using patient blood samples, this study showed that N-PCR is much more sensitive than C-PCR for the diagnosis of scrub ...Q-PCR using patients’ blood, Q-PCR had 83% ... See full document
6
Development and validation of a duplex real time PCR assay for the diagnosis of equine piroplasmosis
... blood using the DNeasy Blood and Tissue Kit (Qiagen, Hilden, ...removing PCR inhibitors present in blood from DNA ...erythrocytes using RBC Lysis Solution (Qiagen, Germantown, MD, USA) followed by ... See full document
12
A real time PCR assay on blood for diagnosis of invasive candidiasis in immunocompromised patient
... difficult. Diagnosis based on clinical symptoms and cultures are non-specific and lack of sufficient sensitivity for the early diagnosis of ...early diagnosis of IC are present but these current ... See full document
7
Novel Technique of Quantitative Nested Real Time PCR Assay for Mycobacterium tuberculosis DNA
... The diagnosis of tuberculous meningitis (TBM) remains a complex issue because the most widely used conventional diagnostic tools, such as culture and PCR assay for cerebrospinal fluid (CSF) samples, ... See full document
11
Quantification of Hepatitis Delta Virus RNA in Serum by Consensus Real Time PCR Indicates Different Patterns of Virological Response to Interferon Therapy in Chronically Infected Patients
... The diagnosis of HDV infection usually relies on the detection of specific anti-HDV antibodies, with the pres- ence of anti-HDV immunoglobulin M (IgM) reflecting ongo- ing viral ...the serum, except in ... See full document
7
Recovery of Cryptosporidium from spiked water and stool samples measured by PCR and real time PCR
... analysis using molecular ...bovine serum albumin (BSA) in PCR mixes at ten different ...concentrated using two methods, and unconcentrated. Nested PCR and a TaqMan nested ... See full document
9
Real Time PCR Assay for Diagnosis of Entamoeba histolytica Infection
... of real-time-PCR, antigen detection, and tra- ditional-PCR assays on stool and liver abscess pus ...traditional nested PCR, and real-time PCR for 205 stool ... See full document
5
Real time PCR applications for diagnosis of leishmaniasis
... each assay has pros and cons, it is difficult to identify an ideal ...a nested approach can be used to enrich the target sequence before the application of species-specific qPCR assays, therefore avoiding ... See full document
13
Bartonella koehlerae, a New Cat Associated Agent of Culture Negative Human Endocarditis
... htrA PCR products of three other specimens from patients with endocarditis, of which two (spec- imens 264271A and 264044) were infected with ...only Bartonella species for which an htrA sequence is ... See full document
7
Development and evaluation of a real time one step Reverse Transcriptase PCR for quantitation of Chandipura Virus
... The real-time RT-PCR assay was performed using the real- time one step RT-PCR master mix (Applied Biosystems International, Foster City, ...The ... See full document
7
Multiplex Real Time PCR shortTUB Assay for Detection of the Mycobacterium tuberculosis Complex in Smear Negative Clinical Samples with Low Mycobacterial Loads
... our assay. Moreover, the multiplex real-time PCR-short TUB assay could be developed with RNA from biological samples in which ... See full document
8
PCR Detection of DNA Specific forTrichosporon Species in Serum of Patients with Disseminated Trichosporonosis
... early diagnosis is crucial for the treatment of this ...novel nested-PCR assay that detects DNA specific for clinically important strains of Trichosporon in serum samples from patients ... See full document
6
Multiplex PCR To Diagnose Bloodstream Infections in Patients Admitted from the Emergency Department with Sepsis
... the PCR results, blood culture was superior to PCR (sensitivities, ...which PCR identified an ...by PCR in a 33-year-old woman who presented for the evaluation of fever, abdominal pain, and ... See full document
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