[PDF] Top 20 Differential Effects of Human Immunodeficiency Virus Type 1 Vpu on the Stability of BST-2/Tetherin

... mature BST-2 (8) or took into account only the dominant immature form (7, ...(i) virus- encoded Vpu increases the rate of turnover of total BST-2 in infected HeLa ...of ... See full document

... protein BST-2 (HM1.24/CD317/tetherin) potently inhibits the release of viral particles and is counteracted by HIV-1 Vpu, which induces its downregulation from the cell surface (27, ... See full document

... of Vpu affect the subcellular localization of the ...that Vpu is localized in the TGN and is associated to some extent with late endosomal compartments suggests that the protein is trafficking through the ... See full document

... Fig. 1 and 2). Depletion of CD4 1 T cells is observed during the course of HIV disease progression, while major fluctuations of monocytes are not ...CD4 1 -T-cell count (mean, 116 m l 21 ), ... See full document

... unpurified virus stocks can significantly interfere with the infection of macrophage cultures and produce aberrant ...of virus stocks (48, 65), contributes to the observed inhibition of virus ...of ... See full document

... of tetherin by HIV-2 Env. Since the HIV-2 Env has been reported to have a Vpu-like function, we first sought to demonstrate that it was capable of rescuing the release of Vpu-defective ... See full document

... Efficient tetherin antagonism may increase viral replicative fitness by several ...of tetherin from the sites of virion budding enables efficient progeny virus release and spread of infection within and ... See full document

... for 1 h followed by 50-fold dilution before infection inhibited HIV replication significantly via a prereverse transcription mechanism ...(Fig. 2), while retrocyclin has little cytotoxicity at peptide ... See full document

... a vpu ORF, such as HIV-2, visna virus, and Moloney murine leukemia virus (5, ...that Vpu may con- tribute to higher viral loads in macaques infected with SHIV (23), no information ... See full document

... h: human; BST-2: bone marrow stromal cell antigen-2; HIV-1: human immunodeficiency virus type 1; Vpu: viral protein U; AGM: African green ... See full document

... during virus assembly, or their processing during virus matu- ...However, effects of Vif on Gag or Gag-Pol processing are not consistently observed in vif mutant virions (8, 22, 43, 48), suggesting ... See full document

... the Vpu protein. Multiple clones of R5 Vpu showed it to be 82 amino acids in length with two additional amino acids at the N-terminus and a deletion at residue 67 compared to NL ...C Vpu proteins ... See full document

... HIV-1 Vpu. agmBST-2 differs from hBST-2 at 44 amino acid positions, with 13 of these clustered in the N- terminal cytoplasmic domain ...confer Vpu resistance on ...These ... See full document

... by Vpu identified ...by Vpu in a ␤ TrCP-dependent manner, a comparative proteomics screening was performed using HeLa-CD4 cells, which are an HIV-susceptible, restric- tive cell ...wild-type ... See full document

... of tetherin in the TGN is associated with antagonism (7, 14, 17, ...our Vpu TM mutants were aberrantly localized. HeLa cells transfected with Vpu-HA or TM mutants were grown on coverslips, fixed 24 h ... See full document

... ⌬ Vpu-infected (black) cells for 6 h or 24 h and subsequent intracellular Gag ...⌬ Vpu (black bars) and either left untreated or pretreated with 500 U/ml interferon for 24 h, mixed with uninfected target T ... See full document

... wt Vpu present in SHIV KU-2MC4 failed to counteract pig-tailed macaque BST-2 ...of Vpu in SHIV KU-2MC4 did not affect virus release due to the presence of SIV Nef, which antagonizes ... See full document

... the virus-containing supernatants were harvested, and virus particles present in the culture medium were ...pelleted virus fractions, separated on SDS–12% polyacrylamide gels, and visualized by ... See full document

... In the case of TRIM5 ␣ s from rhesus macaque, squirrel mon- key, and owl monkey, and probably for other TRIM5 ␣ s, it appears that a major site of interaction between TRIM5 and retroviral capsid is at the exposed loop ... See full document

... The amounts of the envelope glycoproteins gp160 and gp120 present in HeLa-T4 cell lysates transfected with HXBH10-vpu+/env+ and HXBH10-vpu-/env+ DNAs are similar in lysates of cells labe[r] ... See full document