[PDF] Top 20 Differentiation of zebrafish spermatogonial stem cells to functional sperm in culture

... mutant zebrafish and maintained for 1 to 16 ...and sperm, indicating that SSCs in hyperplasia continued to proliferate and differentiate in the adult ... See full document

... embryonic stem cell (ESC) differentiation, growth, and maintenance of undifferentiated ...the culture of human testicular cells under ESCs conditions resulted in the formation of SSC ...the ... See full document

... testicular cells. Culture on extracellular matrix (ECM) has been observed to lead to initiation of differentiation in spermatogonial stem cells ...SSCs differentiation. ... See full document

... SSC culture and spermatogenesis in vitro Much of our knowledge about stem cell regulation in many tissues has been gathered by culturing stem cells under various conditions and studying their ... See full document

... of functional DA neurons as demonstrated by significant dopaminergic marker expression and func- tional properties after 2 and 3 weeks of in vitro ...neural differentiation, survival, maturation, and ... See full document

... Sertoli cells to form seminiferous tubule structures in the testes of infertile recipients with busulfan-compro- mised or genetically defective endogenous Sertoli cells was reported ...Sertoli ... See full document

... permatogonial stem cells (SSCs) are the foundation of spermatogenesis and male ...specific stem cells, SSCs are rare, representing only ...germ cells in rodent testis ...and ... See full document

... cultured cells retained spermatogonial stem cell activity, we used a spermatogonial transplantation technique that allows competent spermatogonial stem cells to colonize ... See full document

... Spermatogonial stem cells have unique properties to self-renew and support spermatogenesis throughout their ...for spermatogonial stem cells, the molecular mechanism of ... See full document

... epiblast cells, in which Oct4 is not expressed, into pluripotent ESCs ...defined culture con- dition can reprogram somatic cells into pluripotent cells ...from stem cells to ... See full document

... dimensional culture systems improve cell proliferation ...and differentiation of animal and human SSCs [15–17]. The soft agar culture system (SACS) is a qualitative, three- dimensional cell ... See full document

... testis cells was first accomplished in the mouse in 1994, with a goal of developing a functional assay to unequivocally define the spermatogonial stem cell (Brinster and Zimmermann, 1994; ... See full document

... of culture and a simple medium was then used with added ...the culture system to the original SSC ...Sertoli cells were used as a feeder layer and GDNF was added, and the least time in the Guan et ... See full document

... University. Cells were harvested via trypsinization, washed in PBS, and resuspended in culture medium with or without ...5 cells were mixed with 40 mg of hydroxyapatite–tricalcium phosphate granules ... See full document

... permeabilized and blocked with blocking solution for 1 hour. The composition of the blocking solution was 1% BSA+6% FBS+ 0.1%TritonX100 in PBS. Primary antibody was added and incubated for 1 hour. Cells were ... See full document

... The importance of the miRNAs for directing the expression of genes essential for spermatogenesis has been highlighted by several studies (31). Male germ cell-specific miR-17-92 knockout in mice led to mild defect in ... See full document

... epithelial cells and hepatoblasts and HNF3 β , which is a liver-enriched transcription factor (Ishizaka et ...during differentiation and endodermal and hepatocyte-specific genes were upregulated upon ...of ... See full document

... Wnt5a mediates effects on DA differentiation via nonca- nonical signaling across rodent species. (A and B) Effects of Wnt5a overexpression were abolished by using a Wnt5a- blocking antibody (W5-ab) or the D4476 ... See full document

... polyvalent functional motifs or peptide ligands with defined density and distance between ...certain functional units that promote cell adhesion, proliferation, and ...mesenchymal stem cells ... See full document

... EDK cells produced elevated levels of epidermal growth factor (EGF), granulocyte-macrophage colony-stimulating fac- tor (GM-CSF), platelet-derived growth factor-AA (PDGF-AA), KGF, and HGF when compared with both ... See full document