[PDF] Top 20 DNA DAMAGE IN SPERM DUE TO CRYOPRESERVATION – A STUDY IN RATS

... C, and this suspension was dropped onto the first agarose layer. Slides were allowed to solidify for 20min at 4 0 C and the slides were immersed in freshly prepared cold lysis buffer. The slides were then incubated at 37 ... See full document

... (CEROS) sperm analyzer ...fittest sperm with intact DNA will fertilize the ovum however sperm with damaged DNA may also achieve fertilization but this is proposed to result in ... See full document

... of sperm depends not only on the functional competence of spermatozoa but also on sperm DNA integrity, 6-8 Sperm chromatin condensation and stability which can detect the sperm ... See full document

... of sperm DNA fragmentation so this test is being more and more recommended especially when patients have to benefit from an ART (Assisted Reproductive Technology) ...our study was to assess if our ... See full document

... like DNA damage, mutagenesis, carcinogenesis and which is also associated with free radical propagation in biological ...current study declared, treatment of CCl 4 -intoxicated rats with ... See full document

... measure sperm DNA defects in human spermatozoa ...and sperm chromatin structure assay (SCSA(7-12)) can evaluate the integrity of sperm ...the sperm chromatin dispersion test (SCD) (13), ... See full document

... another study, Zheng et ...our study, leptin did not change pro- oxidant ...be due to a rise of antioxidant enzymes ...present study, leptin also poten- tiated DNAf reduction in frozen-thawed ... See full document

... present study was showed the negative influence of cryopreservation on sperm parameters especially the progresseve motility and normal morphology for sperms ...of sperm and the addition of the ... See full document

... in rats induced by protein-de fi cient ...induce DNA fragmentation in testis, accompanied by the suppression of the p53 and PCNA- related apoptotic ...spermatogenesis, sperm parameters and in vitro ... See full document

... to damage by LPO ...can damage DNA and proteins, either through oxidation of DNA bases (primarily guanine via lipid peroxyl or alkoxyl radicals) or through covalent binding to MDA resulting in ... See full document

... their sperm cells, improving motility, particu- larly in asthenospermic patients ...previous study, an inverse relationship of sperm count and viability was seen in serum and seminal zinc levels, ... See full document

... as DNA, proteins and lipids become oxidized, resulting in lipid peroxidation, DNA damage and modulation of antioxidant system [39-40], gene alteration [41], cell proliferation [42], chromosomal ... See full document

... (LIFE) study was a prospective, observational cohort study that assessed 501 couples discontinuing contraception with the goal of becoming pregnant with TTP as the primary end- ...LIFE study ... See full document

... to sperm proteomic profile ...on sperm, and influences sperm motility and fertilizing ability ...present study, the GO analysis revealed that the cellular locali- zations of the identified ... See full document

... This study was designed to evaluate the protective effect of methanolic (SCM), ethanolic (SCE) and aqueous (SCA) extracts of Syzygium cumini seeds on arsenic- induced blood cell genotoxicity and hepatotoxicity in ... See full document

... the DNA compaction and thus exposing the DNA to further damage ...on sperm physiology. It is needed a deeper study of what ROS and their levels, and what antioxidant enzymes are im- ... See full document

... this study for the vitrification of dog sperm (fresh and swum-up sperm), different cryopreservation mediums on the basis of glycerol, milk and egg yolk were ...of sperm parameters. The ... See full document

... dangerous damage caused by IR, posing a serious threat to cell viability and genome ...of DNA double-strand breaks (DSBs), was detected by FCM and western ...residual DNA damage, we further ... See full document

... mortality due to cutaneous melanoma (CM) in ...to study patient and tumor characteristics among Norwegian patients, their relations to melanoma- specific death, and compare our results with those from other ... See full document

... during cryopreservation is 0 to −60°C, especially 0 to −10°C, because intercellular ice accumulates at these temperatures and cell damage oc- curs within this temperature zone during the process of cooling ... See full document