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[PDF] Top 20 Effect of Multimerization on Membrane Association of Rous Sarcoma Virus and HIV-1 Matrix Domain Proteins

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Effect of Multimerization on Membrane Association of Rous Sarcoma Virus and HIV-1 Matrix Domain Proteins

Effect of Multimerization on Membrane Association of Rous Sarcoma Virus and HIV-1 Matrix Domain Proteins

... chimeric proteins was examined by fluorescence imaging, and the association of the proteins with liposomes was quantified by flotation in sucrose gradients, following synthesis in a reticulocyte ... See full document

11

RNA Dimerization Defect in a Rous Sarcoma Virus Matrix Mutant

RNA Dimerization Defect in a Rous Sarcoma Virus Matrix Mutant

... Src membrane-binding domain at the N terminus of ...components, virus particles were examined for incorporation of Env glycoproteins, genomic RNA, and Gag and Gag-Pol ...plasma membrane- ... See full document

9

Membrane Binding of the Rous Sarcoma Virus Gag Protein Is Cooperative and Dependent on the Spacer Peptide Assembly Domain

Membrane Binding of the Rous Sarcoma Virus Gag Protein Is Cooperative and Dependent on the Spacer Peptide Assembly Domain

... underlying membrane binding and assembly of retroviral Gag proteins into a lattice are ...purified Rous sarcoma virus Gag and Gag truncations, we studied the interrelation of Gag-Gag ... See full document

13

Specificity of Plasma Membrane Targeting by the Rous Sarcoma Virus Gag Protein

Specificity of Plasma Membrane Targeting by the Rous Sarcoma Virus Gag Protein

... plasma membrane sites for budding remains elusive, we have undertaken the study of RSV Gag mutants to identify regions of the M domain that are involved in specific plasma membrane ...in ... See full document

11

Lysines Close to the Rous Sarcoma Virus Late Domain Critical for Budding

Lysines Close to the Rous Sarcoma Virus Late Domain Critical for Budding

... Gag proteins of human immu- nodeficiency virus type 1 (HIV-1) and murine leukemia virus (MLV) has no effect on budding or infectivity ...Gag proteins could ... See full document

11

Hydrodynamic and Membrane Binding Properties of Purified Rous Sarcoma Virus Gag Protein

Hydrodynamic and Membrane Binding Properties of Purified Rous Sarcoma Virus Gag Protein

... into virus-like particles in vitro, we infer that RSV Gag is biologically ...unmyristoylated HIV-1 Gag, which is ...unlike HIV-1 Gag, RSV Gag shows no sensitivity to acyl chain ...MA ... See full document

12

In Vivo Interference of Rous Sarcoma Virus Budding by cis Expression of a WW Domain

In Vivo Interference of Rous Sarcoma Virus Budding by cis Expression of a WW Domain

... plasma membrane (as in the internal chimera), this nonspecific effect was ...WW-L domain interaction in vivo, they do not shed light on the actual host proteins that are recruited to the sites ... See full document

7

Repositioning Basic Residues in the M Domain of the Rous Sarcoma Virus Gag Protein

Repositioning Basic Residues in the M Domain of the Rous Sarcoma Virus Gag Protein

... M domain can also impair ...leukemia virus and HIV-1 tolerate sub- stitutions of four or more basic residues (9, 33, ...an HIV-1 Gag mutant lacking all but one basic residue in ... See full document

8

Mechanistic Differences between Nucleic Acid Chaperone Activities of the Gag Proteins of Rous Sarcoma Virus and Human Immunodeficiency Virus Type 1 Are Attributed to the MA Domain

Mechanistic Differences between Nucleic Acid Chaperone Activities of the Gag Proteins of Rous Sarcoma Virus and Human Immunodeficiency Virus Type 1 Are Attributed to the MA Domain

... NC domain is the primary NA interaction domain for both proteins, the Gag polyprotein is able to multimerize due to the CA domain (13, 50), and it is pos- sible that these intermolecular ... See full document

10

Self-assembly in vitro of purified CA-NC proteins from Rous sarcoma virus and human immunodeficiency virus type 1.

Self-assembly in vitro of purified CA-NC proteins from Rous sarcoma virus and human immunodeficiency virus type 1.

... viral proteins must interact with each other, with the RNA genome, with the plasma membrane, and at least in some cases with cellular ...avian sarcoma and leukemia viruses (whose prototype is ... See full document

11

Positionally independent and exchangeable late budding functions of the Rous sarcoma virus and human immunodeficiency virus Gag proteins.

Positionally independent and exchangeable late budding functions of the Rous sarcoma virus and human immunodeficiency virus Gag proteins.

... the HIV membrane-binding domain (2, 17) and also the small membrane-binding domain of the Src oncoprotein, pp60 src ...Gag proteins of HIV (2), murine leukemia ... See full document

6

PR Domain of Rous Sarcoma Virus Gag Causes an Assembly/Budding Defect in Insect Cells

PR Domain of Rous Sarcoma Virus Gag Causes an Assembly/Budding Defect in Insect Cells

... and HIV-1 Gag was soluble in these conditions, over 60% of the Myr2 protein was insoluble, as evidenced by ability to be collected by cen- trifugation for 5 min at 14,000 ⫻ g (data not ...Myr2 ... See full document

6

An LYPSL Late Domain in the Gag Protein Contributes to the Efficient Release and Replication of Rous Sarcoma Virus

An LYPSL Late Domain in the Gag Protein Contributes to the Efficient Release and Replication of Rous Sarcoma Virus

... the virus to recruit the budding machinery to the site of assembly and thereby results in a block at the late stage of virus release in which fully assembled but immature virus particles remain ... See full document

12

Rous Sarcoma Virus Gag Has No Specific Requirement for Phosphatidylinositol-(4,5)-Bisphosphate for Plasma Membrane Association In Vivo or for Liposome Interaction In Vitro

Rous Sarcoma Virus Gag Has No Specific Requirement for Phosphatidylinositol-(4,5)-Bisphosphate for Plasma Membrane Association In Vivo or for Liposome Interaction In Vitro

... While HIV-1 was intended primarily as a positive control, our results differ in some respects from those published previ- ...abrogated HIV-1 Gag-GFP PM localization, as first found in the ... See full document

10

The membrane-binding domain of the Rous sarcoma virus Gag protein.

The membrane-binding domain of the Rous sarcoma virus Gag protein.

... FIG. 1. Schematic diagrams of chimeras. The wild-type Gag and Src proteins are depicted at the top by white and gray bars, ...Myr1-Src proteins represent ...The proteins were expressed by ... See full document

5

Membrane Proteins of Uninfected and Rous Sarcoma Virus-Transformed Avian Cells

Membrane Proteins of Uninfected and Rous Sarcoma Virus-Transformed Avian Cells

... amount of radioactive MP-1 relative to other proteins in membrane preparations from unin- fected cells was the same after incubation of. uninfected cells with 3H-amino acids for times fr[r] ... See full document

8

Functional chimeras of the Rous sarcoma virus and human immunodeficiency virus gag proteins.

Functional chimeras of the Rous sarcoma virus and human immunodeficiency virus gag proteins.

... Failure of a human immunodeficiency virus (HIV) immune globulin to protect chimpanzees against experimental challenge with HIV. Myristylation site in PM5`' is essential for virus particl[r] ... See full document

12

Insertion of a Classical Nuclear Import Signal into the Matrix Domain of the Rous Sarcoma Virus Gag Protein Interferes with Virus Replication

Insertion of a Classical Nuclear Import Signal into the Matrix Domain of the Rous Sarcoma Virus Gag Protein Interferes with Virus Replication

... NLS-containing virus parti- cles yielded intriguing results. The RC.V8.NLS virus particles appeared to be normal in their Gag protein processing, Gag- Pol incorporation, Env packaging, and viral RNA ... See full document

9

Comparison of Rous Sarcoma Virus-Specific Deoxyribonucleic Acid Polymerases in Virions of Rous Sarcoma Virus and in Rous Sarcoma Virus-Infected Chicken Cells

Comparison of Rous Sarcoma Virus-Specific Deoxyribonucleic Acid Polymerases in Virions of Rous Sarcoma Virus and in Rous Sarcoma Virus-Infected Chicken Cells

... on the nature of the core of Rous sarcoma virus (RSV) virions and of particles in RSV-infected chicken cells which contain endogenous viral RNA-directed and exogenous DNA-directed DNA po[r] ... See full document

10

Genetic analysis of interactions between Gag proteins of Rous sarcoma virus.

Genetic analysis of interactions between Gag proteins of Rous sarcoma virus.

... Gag proteins of several other retrovi- ruses, including HIV-1 and ...each virus has evolved a very specific set of contact surfaces that are generally not conserved well enough to mate with ... See full document

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