[PDF] Top 20 An efficient calcium phosphate nanoparticle-based nonviral vector for gene delivery

... classical calcium phosphate ...classical calcium phosphate ...the delivery of DNA into cells through endocytosis and could improve transfection ...classical calcium ... See full document

... and efficient delivery platform without ...RNA nanoparticle (RNP) based on pRNA 3-way-junction (3WJ) from bacteriophage phi29 to target glioblastoma cells with folate (FA) ligand and deliver ... See full document

... of gene expression of the ampli- con was significantly enhanced when used with a helper virus ...effective gene delivery is essential, it may be advantageous to use a helper virus containing amplicon ... See full document

... the calcium deposit in mineralized tissues and ...extracellular calcium and phosphate ...the calcium deposit. Based on these results, chitosan-g- PEI-mediated BMP2 gene ... See full document

... their delivery, stability, and off-target effects. Delivery systems provide solutions to many of the challenges facing siRNA ...vectors, nonviral carriers have been studied ...for gene ... See full document

... Calcium phosphate nanoparticles, routinely used for in vitro transfection, have been investigated as a power- ful non-viral gene delivery ...or nonviral), show good gene ... See full document

... it remains extrachromosomal and directs a transient transgene expression. On the other hand, polylysine complexes com- bined with plasmid DNA (pDNA/ cationic polymer complexes) offer a chemical method of gene ... See full document

... Gene delivery approaches provide a mechanism to dir- ectly alter gene expression within a cell ...current delivery systems have limited the use of gene delivery for therapeutic, ... See full document

... viral gene vectors are commonly utilized to construct TRAIL-expressing stem cells that have promising apoptosis-inducing effects on human cancer cells [11, ...their efficient gene delivery ... See full document

... effective nonviral gene delivery system against avian influenza virus (AIV) that induced cytokine expression, the hemagglutinin (H5) gene of AIV, A/Ck/Malaysia/5858/04 (H5N1) and green ... See full document

... for gene delivery research in the ...effective gene delivery nanocarriers in the near ...different gene delivery systems should be effectively combined to make best use of the ... See full document

... novel gene vector, cationized PPS (CPPS) nanoparticles, for the nonviral gene delivery of a plasmid DNA encoding Wnt3a into human umbilical cord mesenchymal stem cells (HUMSCs) in ... See full document

... purchased from Sigma-Aldrich (St Louis, MO, USA). N,N′ - carbonyldiimidazole (CDI), DMAPA, AEPZ, and dimethyl sulfoxide (DMSO) were acquired from Aladdin Reagent (Shanghai, People’s Republic of China). DMSO was dried for ... See full document

... Hep G2 (human hepatocellular carcinoma; American Type Culture Collection [ATCC] No HB-8065™), SMMC- 7721 (human hepatocellular carcinoma), MCF-7 (human breast adenocarcinoma; ATCC No HTB-22™), AGS (human gastric ... See full document

... Agricultural productivity relies on many factors including the type of soil, seeds, optimum dose fertilizers, their mode of application, availability of water for irrigation and climatic factors. To increase productivity ... See full document

... chronic phosphate depletion on renal tubular function were evaluated by micropuncture and free water clearance studies in the ...in phosphate-depleted dogs, with proportional inhibition of calcium ... See full document

... intranasally. Based on these results, and as of June 2007, Alnylam has initiated a phase II trial with ALN-RSV01 that is designed to evaluate the safety, tolerability, and antiviral activ- ity of ALN-RSV01 in  ... See full document

... the delivery of a DNA vaccine model in vitro and in vivo based on pHEMA + chitosan nanospheres, exploiting their core–shell interaction, which showed high transfection capability through interaction with ... See full document

... fection efficiency of PPs rapidly increased with the increase in ratio of PEI 2KD/Pluronic, which can be explained by the high degree of modification of PEI 2KD leading to an increase in positive surface and structural ... See full document

... ) showed very high site-specific recombinase activity, since 491-bp fragments, ex- pected from template DNA molecules that went through Cre/ loxP-mediated excision, were the strikingly prominent species (Fig. 1B). ... See full document