[PDF] Top 20 Efficient CRISPR/Cas9 genome editing with low off target effects in zebrafish
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Efficient CRISPR/Cas9 genome editing with low off target effects in zebrafish
... the CRISPR/Cas9 system we speculated that it might be suitable to facilitate a targeted KI approach in ...injected Cas9 mRNA and the gRNA targeting the ATG start codon of C13H9orf72 (C9t3) together ... See full document
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Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease
... the CRISPR/Cas9 system and developed methods for one-step generation of mice carrying mutations in a single or multiple genes, as well as mice carrying reporter and conditional alleles (Wang et ...and ... See full document
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Upgrading of efficient and scalable CRISPR–Cas-mediated technology for genetic engineering in thermophilic fungus Myceliophthora thermophila
... Cas12a-based CRISPR system for efficient multiplex genome editing, using a single-array approach in ...These CRISPR–Cas12a cassettes worked well for simultaneous multiple gene ...the ... See full document
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Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off target effects
... of Cas9-mediated DNA cleavage and tolerates fewer mismatches than the PAM-distal sequences [19, 27, ...the CRISPR/Cas9 system for cattle genome editing, we developed an evaluation model ... See full document
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Towards a CRISPR view of early human development: applications, limitations and ethical concerns of genome editing in human embryos
... the CRISPR-Cas9 system was initially realized, ethical concerns were raised about the possibility of creating permanent and inheritable changes in the genome of human gametes and ...involving ... See full document
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Keeping CRISPR/Cas on-Target
... of Cas9 (Fu et ...human genome editing was achieved by tru-gRNAs(Fu et ...The off-target mutagenesis was largely reduced about 5000-fold by using tru-gRNA (Fu et ...of cas9 ... See full document
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Development of a CRISPR-Cas9 System for Efficient Genome Editing of Candida lusitaniae
... the CRISPR-Cas9 system and for those that did ...of CRISPR, diploid cells transformed with the short- FIG 2 Genotypic analysis of red colonies arising from CRISPR-Cas9 targeting of ADE2 ... See full document
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Optimizing CRISPR Cas9 Genome Editing System: A Review
... reduce off target activity by 50-1000 folds in cell lines and facilitated gene knockout in mouse zygotes without sacrificing on-target cleavage efficiency 21 ...of Cas9 and sgRNAs into the ... See full document
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Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium glutamicum
... robust genome editing tools are urgently demanded. The CRISPR/Cas9 system that utilized NGG as proto- spacer adjacent motif (PAM) and has good targeting specificity is expected to enable ... See full document
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Multiplexed CRISPR-Cas9-Based Genome Editing of Rhodosporidium toruloides
... of CRISPR-Cas9-based gene disruption in ...a Cas9 ex- pression cassette harboring nourseothricin resistance and selecting transformants on this antibiotic resulted in strains of ...While ... See full document
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ARNT is a potential direct HIF-1 target gene in human Hep3B hepatocellular carcinoma cells
... loci. Low affinity HIF binding sites might also be occupied by HIF-1 dur- ing prolonged hypoxia ...HIF target genes and contribute to cell-type specific hypoxic ...HIF target assumes that disruption ... See full document
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A Multiplex CRISPR/Cas9 System for Use as an Anti-BmNPV Therapeutic
... one-vector CRISPR/Cas9 system for BmNPV genome excision BmNPV contains 141 genes most of which have not been studied [1, ...the effects of different gene knockouts on the replication of BmNPV, ... See full document
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CRISPR/Cas9 TECHNOLOGY A NEW BOON IN GENOME EDITING
... by efficient DNA assembly techniques such as Golden Gate ...the target DNA sequence, so sophisticated protein engineering for each target is unnecessary and only 20 nt in the gRNA need to be modified ... See full document
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CRISPR-Cas9: from Genome Editing to Cancer Research
... Gene editing is a widely used engineering tool for generating mutations that enhance ...the genome editing approach into a more convenient and efficient ...animals, CRISPR-Cas9 ... See full document
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Efficient and Scalable Precision Genome Editing in Staphylococcus aureus through Conditional Recombineering and CRISPR/Cas9 Mediated Counterselection
... and Cas9-mediated counterselection for ...modified; Cas9 followed by a transcriptional terminator; low-copy-number temperature- sensitive ...and Cas9 (yellow Pac-Man symbol) is unable to cleave ... See full document
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Efficient Editing of Malaria Parasite Genome Using the CRISPR/Cas9 System
... a CRISPR/Cas9 system for modification of the ...yoelii genome. CRISPR/Cas9-mediated genome editing re- quires expression of two components: Cas9 nuclease and a ... See full document
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Highly Specific and Efficient CRISPR/Cas9-Catalyzed Homology-Directed Repair in Drosophila
... the Cas9 nuclease and a single chimeric guide RNA ...20-nt target se- quence next to a trinucleotide NGG protospacer adjacent mo- tif (PAM) in the genome to direct Cas9-mediated cleavage of ... See full document
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Efficient CRISPR–Cas9 mediated multiplex genome editing in yeasts
... of target products [43, 44]. The integration of multi-copy target gene into the genome is an important method for increasing gene expression, especially in the organism without episomal plasmids [28, ... See full document
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In Vitro CRISPR/Cas9 System for Efficient Targeted DNA Editing
... the genome with high efficiency and specificity in vivo (22–27). The CRISPR/Cas system from Streptococcus pyogenes, which consists of Cas9 protein and two RNAs (CRISPR RNA [crRNA] and ... See full document
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CRISPR Cas9 Mediated Genome Editing in Leishmania donovani
... prokaryotic CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9, an RNA-guided endonu- clease, has been shown to mediate efficient genome editing in a wide variety of ... See full document
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