[PDF] Top 20 Nef mediated enhancement of cellular activation and human immunodeficiency virus type 1 replication in primary T cells is dependent on association with p21 activated kinase 2

... of activated PBMC compared to pooled human A/B serum, thereby increasing the number of activated cells and reducing the percentage of resting ...of human A/B serum. We have shown that ... See full document

... impair Nef-mediated enhancement of virion infectivity in P4- CCR5 cells but not in another indicator cell line, TZM-bl, or in human peripheral blood mononuclear cells ...NFAT ... See full document

... accessory human immunodeficiency virus type 1 (HIV-1) protein Nef activates the autophosphor- ylation activity of p21-activated kinase 2 ...a ... See full document

... The Nef-mediated alterations in T-cell function may be sec- ondary to an evolved role for Nef in HIV ...the virus-generating cell, Nef in- creases the infectivity of viral ...to ... See full document

... The Nef protein of human immunodeficiency virus type 1 is an important factor in AIDS ...infectivity, Nef triggers activation of the T-cell receptor (TCR) ... See full document

... 23 nef mutant viruses exhibited significantly im- paired replication in activated CD4 ⫹ primary T ...the nef- defective virus, suggesting that an additional function of ... See full document

... 1997 Nef proteins from human immunodeficiency virus type 1 isolate SF2 (HIV-1 SF2 ) and simian immunodefi- ciency virus isolate mac239 (SIVmac239) have been found ... See full document

... The Nef protein enhances human immunodeficiency virus type 1 (HIV-1) replication through an unknown ...HIV-1 replication in activated ... See full document

... 2004/Accepted 1 September 2004 Infected CD4 ⴙ T cells are the primary sites of human immunodeficiency virus type 1 (HIV-1) replication in ... See full document

... Thus, Nef triggers plasma membrane recruitment of Vav1 and leads to some incorporation of the GEF in plasma membrane ...of Nef, and both proteins were occasionally found to colocalize in membrane ... See full document

... that human immunodeficiency virus type 1 (HIV-1) Nef contributes substantially to disease pathogenesis by augmenting virus replication and markedly ... See full document

... The association between PD-1 expression on HIV-specific T cells, cellular exhaustion, and disease progression may rep- resent an important advance in our understanding of HIV ...immune ... See full document

... protein Nef plays a major role in the pathogenesis of human immunodeficiency virus type I (HIV-1) ...understood, Nef has been shown to be essential for the maintenance of ... See full document

... domain- mediated interactions, makes participation in RT loop or SH3 domain interactions ...of Nef and has the freedom to move between two energetically favorable confo- mers ... See full document

... PAK-2 activation by ...intact cells, possibly allowing for the deter- mination of which proteins implicated in Nef activation of PAK-2 are actually required for this Nef ... See full document

... threonine kinase activity reported to be associated with some Nef ...ent Nef alleles also implicates that these two Nef functions are mechanistically ...Thus, Nef is not mimicking an ... See full document

... of human immunodeficiency virus type 1 (HIV-1), designated nef, encodes a 25 or 27 kDa ...product, Nef, acted as a negative factor in virus ... See full document

... HIV-1 Nef are most closely associ- ated with in vivo pathogenesis in the SCID-hu Thy/Liv mouse ...different Nef mu- tations that compromised CD4 downregulation (WL57AA, LL164AA, and DD174AA) into the ... See full document

... the nef gene can function as a pathogenic factor indepen- dent of replication ...the replication determinants in ...the human thymus. Restoration of the nef gene was required for ... See full document

... University, 2 Nashville, Tennessee Received 16 February 1999/Accepted 16 June 1999 Highly conserved among primate lentiviruses, the human immunodeficiency virus type 1 ... See full document