[PDF] Top 20 Evaluation of Amplicor PCR for direct detection of Mycobacterium tuberculosis from sputum specimens
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Evaluation of Amplicor PCR for direct detection of Mycobacterium tuberculosis from sputum specimens
... 17 PCR-positive, M. tuberculosis culture-neg- ative specimens were from four patients with a history of an- tituberculous ...M. tuberculosis within 2 months of producing ... See full document
5
Relevance of Commercial Amplification Methods for Direct Detection of Mycobacterium tuberculosis Complex in Clinical Samples
... consecutive specimens, the patient can be presumed to be active TB free ...diagnostic evaluation and anti-TB therapy, such as cases when the likelihood of TB is neither very high nor very ... See full document
11
Direct Detection of Rifampin Resistant Mycobacterium tuberculosis in Respiratory Specimens by PCR DNA Sequencing
... the direct application of a PCR-sequencing assay in clinical spec- imens for genotypic prediction of RIF ...with PCR assays for M. tuberculosis identification, our rpoB PCR was 100% ... See full document
6
Rapid and specific detection of Mycobacterium tuberculosis directly from sputum specimens using IS6110 and pncA through multiplex-PCR
... sole laboratory method for the TB diagnosis in addition to radiography. There are no appropriate diagnostic facilities in most of the hospitals in Balochistan. The delay in diagnosis and treatment is one of the major ... See full document
9
Evaluation of conventional and molecular strategies for the rapid diagnosis and molecular characterisation of strains of Mycobacterium tuberculosis
... Comparativestudy of Amplicor for ligase direct detection chain reaction and reaction of polymerasechain M tuberculosis in clinical specimens.. Saudi Medical Journal.[r] ... See full document
212
Direct Drug Susceptibility Testing of Mycobacterium tuberculosis for Rapid Detection of Multidrug Resistance Using the Bactec MGIT 960 System: a Multicenter Study
... of Mycobacterium tuberculosis with liquid medium is well established and of- fers time-saving and reliable ...directly from processed smear-positive specimens (direct DST) and if this ... See full document
6
Evaluation of Gen Probe Amplified Mycobacterium Tuberculosis Direct Test by Using Respiratory and Nonrespiratory Specimens in a Tertiary Care Center Laboratory
... three specimens collected on dif- ferent days be submitted for ...additional specimens should be tested. If the sputum is smear negative and AMTD positive, additional specimens should be ... See full document
5
Evaluation of FLASH - PCR forrapid detection of Mycobacterium tuberculosis from clinical specimens.
... clinical specimens can be rapidly detected using of FLASH-PCR associated with culture and smear ...conventional PCR, while contamination occurs during ... See full document
8
Comparison of the Roche AMPLICOR MYCOBACTERIUM assay and Digene SHARP Signal System with in house PCR and culture for detection of Mycobacterium tuberculosis in respiratory specimens
... M. tuberculosis complex organisms was accomplished by hybrid- ization of the amplified product to a DNA probe specific for organisms of the ...min. Detection of hybridized duplex was accomplished with an ... See full document
5
Evaluation of Roche Amplicor PCR assay for Mycobacterium tuberculosis
... RMtb-PCR-positive specimens. A to- tal of 18 specimens from 16 patients were initially classified as RMtb-PCR false positive with culture as the gold standard (Table ...18 ... See full document
6
Rapid Detection of Mycobacterium tuberculosis and Pyrazinamide Susceptibility Related to pncA Mutations in Sputum Specimens through an Integrated Gene to Protein Function Approach
... gene from a clinical sample is found to have new mutations not available in the current PZA resistance mutation database, the LF-qPCR amplicon is used as the template for ePCR to introduce the expression elements ... See full document
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Rapid and Simultaneous Detection of Mycobacterium tuberculosis Complex and Beijing/W Genotype in Sputum by an Optimized DNA Extraction Protocol and a Novel Multiplex Real Time PCR
... purification from human body ...M. tuberculosis detection directly from sputum specimens; (ii) develop a mul- tiplex real-time PCR for TB diagnosis and simultaneous de- ... See full document
7
Evaluation of the semiautomated Abbott LCx Mycobacterium tuberculosis assay for direct detection of Mycobacterium tuberculosis in respiratory specimens
... M. tuberculosis DNA in sputum was demonstrated ...M. tuberculosis Assay-positive and M. tuberculosis culture-negative specimens were from ... See full document
7
Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by Amplicor PCR
... the PCR test presented here indicate that the majority of M. tuberculosis-positive specimens can be identified rapidly if they are screened by the ...the PCR is not equal to that of culture in ... See full document
6
Loop Mediated Isothermal Amplification for Direct Detection of Mycobacterium tuberculosis Complex, M avium, and M intracellulare in Sputum Samples
... per Amplicor reaction solution were calcu- lated by assuming a molecular size of ...Clinical sputum specimens. Sixty-six sputum specimens from 58 patients with suspected ... See full document
7
Rapid Detection of Mycobacterium tuberculosis in Respiratory Samples by Transcription Reverse Transcription Concerted Reaction with an Automated System
... rapid detection and identification of Mycobacterium tuberculosis complex (MTC) in respiratory samples are extremely important for optimal diagnosis and effective treatment, as well as for prevention ... See full document
5
Direct Detection of Rifampin and Isoniazid Resistant Mycobacterium tuberculosis in Auramine Rhodamine Positive Sputum Specimens by Real Time PCR
... retrieved from the processed sputum samples of 88 different patients were tested (Table ...inferred from the Light- Cycler data when the rpo1 and rpo2 pair of probes was ...real-time PCR when ... See full document
5
Clinical Evaluation of TRCRapid M TB for Detection of Mycobacterium tuberculosis Complex in Respiratory and Nonrespiratory Specimens
... Committee. From December 2006 to November 2008, specimens were obtained from 1,282 patients, including chil- dren and adults, hospitalized in the Yokohama City University Medical Center, Kanagawa, ... See full document
6
Improving the Sensitivity of the Xpert MTB/RIF Assay on Sputum Pellets by Decreasing the Amount of Added Sample Reagent: a Laboratory and Clinical Evaluation
... near-patient detection of Mycobacterium tuberculosis in sputum; however, the test sensitivity remains suboptimal in paucibacillary specimens that are negative for acid-fast bacilli ... See full document
6
Rapid Detection of the Mycobacterium tuberculosis Complex by Use of Quenching Probe PCR (geneCube)
... Early detection of tuberculosis (TB) is essential for infection ...detect Mycobacterium tuberculosis complex (MTBC) and Mycobacterium avium complex (MAC) in clinical ...Cobas ... See full document
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