[PDF] Top 20 Evaluation of the Analytical Performance of the Xpert MTB/RIF Assay

... genome copies per reaction. Negative controls contained no DNA. Initial DNA concentrations were determined with a NanoDrop ND-1000 (Thermo Scientific) and diluted in TET buffer. When reliable genome size was not ... See full document

... The Xpert MTB/RIF (Xpert) assay permits rapid near-patient detection of Mycobacterium tuberculosis in sputum; however, the test sensitivity remains suboptimal in paucibacillary ... See full document

... with MTB than ...of MTB cases, but the lowest number of MTB cases was re- corded among the age group of below 20 years (see Table ...249 MTB-positive males, 190 (76.3%) tested posi- tive for ... See full document

... in RIF resistance [24-26]. In this study, 7 isolates were identified as RIF-resistant by the Xpert assays; 6 of these isolates had confirmed resistance according to culture-based ...for RIF ... See full document

... Xpert MTB/RIF is a TB-specific automated, car- tridge-based nucleic amplification assay with fully integrated and automated sample prepa- ration, amplification and detection of Mtb, as ... See full document

... quantitative Xpert MTB/RIF assay readouts for sputum M tuberculosis DNA as a biomarker correlate well with M tuberculosis smear and culture results during antituberculosis ...with Xpert ... See full document

... TB: Xpert MTB/RIF assay, Cepheid, USA; TB: tuberculosis; MTB: Mycobac- terium tuberculosis; KJ: Kenneth-Jones; GA: gastric aspirates; Spm: sputum; TRS: treatment response standard; PPV: ... See full document

... an evaluation of a newly developed molecular assay for the detection of cell-free Mycobacterium tuberculosis ...the Xpert MTB/RIF assay, the adenosine deaminase assay, the ... See full document

... and Xpert MTB/RIF ...and Xpert MTB/RIF assay but negative by a reference laboratory ...the evaluation NAATs were performed on a decontaminated and concen- trated ... See full document

... The Xpert MTB/RIF assay was endorsed for use on patient material, regardless of smear gradation, while the GenoType MTBDRplus (version 1) has been limited for use on smear-positive patient ... See full document

... the performance of the Xpert assay in suspected musculoskeletal TB samples which were tissue or pus taken from bones, joints, or spine, but the individual reference standard to assess the diagnostic ... See full document

... INNO-LiPA RIF TB (Innogenetics, Ghent, Belgium) and the Genotype MTBDRplus (Hain Life-Sci- ence, Nehren, Germany) (here referred as to ...and Xpert MTB/RIF assays have shown good ... See full document

... the assay to detect 16 CFU/ml, in contrast to the 114-CFU/ml limit of detection of Xpert MTB/RIF ...the assay, such as a larger PCR chamber in the cartridge, appear to have improved its ... See full document

... [16]. Performance of Xpert MTB/RIF in tuberculosis spondylitis has been reported in very few ...disease, Xpert MTB/RIF had a sensitivity of ...using Xpert ... See full document

... of MTB, the impact of preceding antibiotic use, and as was seen in the FIND trial but not in our study, the persistence of nonviable mycobacterial DNA in patients with a history of treated ...(Xpert ... See full document

... the Xpert MTB/RIF sample ...the Xpert MTB/RIF ...An Xpert MTB/RIF test was considered positive if MTB was detected and negative if MTB was not ... See full document

... T uberculosis (TB) is one of the leading causes of death from an infectious disease worldwide (1). Diagnostic delays contribute significantly to death. In the case of pulmonary TB (PTB), a diag- nosis can often be made ... See full document

... the Xpert MTB/RIF ...the Xpert MTB/RIF Ultra assay in our cohort, and this test may have higher sensitivity than current diagnostic tests ...diagnostic performance ... See full document

... the evaluation of medical history, clinical evaluation with chest radiography, tuberculin skin-testing and smear mi- croscopy with mycobacterial culture where available ...the Xpert ... See full document

... the Xpert MTB/RIF assay with better TB detection capabilities and more definitive identification of RIF susceptibility and resis- ...in analytical sensitivity for the detection of ... See full document