[PDF] Top 20 Evaluation of denaturing gradient gel electrophoresis (DGGE) as a method of mutation detection
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Evaluation of denaturing gradient gel electrophoresis (DGGE) as a method of mutation detection
... same mutation and heterozygote advantage may be the mechanisms that best explain the present distribution of m utant PAH ...deletion mutation in exon 3 of the PAH gene which is observed in a large ... See full document
207
A Comparison Between Denaturing Gradient Gel Electrophoresis and Denaturing High Performance Liquid Chromatography in Detecting Mutations in Genes Associated with Hereditary Non-Polyposis Colorectal Cancer (HNPCC) and the Identification of 9 New Mutations Previously Unidentified by DGGE
... new method by which heteroduplex structures formed during the PCR amplification of heterozygote samples can be rapidly ...for mutation detection in hereditary non-polyposis colorectal cancer (HNPCC) ... See full document
10
Evaluation of Denaturing Gradient Gel Electrophoresis (DGGE) for the Determination of Marine Phytoplankton Genetic Diversity
... is gradient gel electrophoresis (GGE), using either a chemical denaturant gradient (DGGE), or a heat gradient ...from DGGE methods optimization using clonal laboratory ... See full document
11
Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities
... as DGGE (Riesner et ...in DGGE, the resolution of TGGE is also mostly dependent upon gel ...an electrophoresis step less than 6 h compared to more than 14 h for ...of DGGE and TGGE ... See full document
11
Evidence of melanoma in wild marine fish populations
... agarose gel electrophoresis ...nator. DGGE was performed using the D-Code universal mutation detection system ...(denaturant) gradient for 13 h at 60uC and a constant voltage of ... See full document
7
TP53 hotspot mutations are predictive of survival in primary central nervous system lymphoma patients treated with combination chemotherapy
... DGGE: denaturing gradient gel electrophoresis; DLBCL: diffuse large b-cell lymphoma; FFPE: formalin fixed paraffin embedded; HD-MTX: high-dose methotrexate; HR: hazard ratio; IARC: ... See full document
13
An investigation into the potential impacts of ocean acidification and ocean fertilisation on the genetic diversity of marine bacterial assemblages
... communities. Denaturing gradient gel electrophoresis (DGGE), described in detail in Section ...2001). DGGE can also be used to screen large clone libraries and remove the ... See full document
277
Metataxonomic profiling and prediction of functional behaviour of wheat straw degrading microbial consortia
... ing gradient gel electrophoresis (DGGE) analyses and isolation of some key consortium ...oxidation method to detect 5-hydroxymethylfurfural oxidoreductase ...metataxonomic ... See full document
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Analysis of the bacterial community in chronic obstructive pulmonary disease sputum samples by denaturing gradient gel electrophoresis and real-time PCR
... The DGGE gel images were analyzed using Phoretix 1D (Single Gel Dendrogram) software (Phoretix, New- castle upon Tyne, UK) ...group method with arithmetic averages (UPGMA) ... See full document
7
Denaturing gradient gel electrophoresis analysis of archaeal and bacterial populations in a submerged anaerobic membrane bioreactor treating landfill leachate at low temperatures
... the DGGE technique as described in Material and Methods, and the gel picture is shown in ...the gel was found to have a high similarity (100 %) to the sequence of Methanosarcina ...the gel. ... See full document
19
Statistical Analyses of Complex Denaturing Gradient Gel Electrophoresis Profiles
... Characteristic profiles of microbial communities or DNA fingerprints can be produced by DGGE. These microbial fin- gerprints can be represented as binary vectors, which have the potential to be of a very high ... See full document
8
Diversity of Hepatitis C Virus Quasispecies Evaluated by Denaturing Gradient Gel Electrophoresis
... Denaturing gradient gel electrophoresis (DGGE) was used to study the diversity of hepatitis C virus (HCV) ...Optimized DGGE running conditions were applied to screen for ... See full document
12
Polymerase Chain Reaction Denaturing Gradient Gel Electrophoresis (PCR DGGE) Profile of Bacterial Community from Agricultural Soils in Bauchi, North East Nigeria
... The need to understand the microbial community that channel soil affair is quite imperative. These will pave way in knowing their activities and mechanis- tic role to the sustenance of ecosystem (e.g. energy flow, ... See full document
7
Elucidation of The Microbial Community in Activated Sludge using PCR-DGGE Analysis in Arid and Semi Arid Regions of Rajasthan
... using denaturing gradient gel electrophoresis (DGGE) of PCR- amplified 16S ribosomal DNA ...from DGGE profiles were excised and subjected to sequencing to identify the dominant ... See full document
11
Diversity of eukaryotic plankton of aquaculture ponds with Carassius auratus gibelio, using denaturing gradient gel electrophoresis
... PCR-denaturing gradient gel electrophoresis (DGGE) fingerprinting was first used for profiling complex microbial populations by Muyzer et ...recognized method for elucidating ... See full document
18
Denaturing Gradient Gel Electrophoresis as a Diagnostic Tool in Periodontal Microbiology
... of DGGE as a diagnos- tic ...the detection of ...the detection of ...or DGGE. The sensitivity of DGGE for ...of detection of the species-specific ...by DGGE than by ...of ... See full document
6
PCR-DGGE analysis of fungal community in manufacturing process of a traditional Iranian cheese
... description, detection, identification and characterization of microorgan- isms in food because they are faster, more reliable and somehow cheaper than conventional culturing method that fail to reproduce ... See full document
7
Phytase producing Bacillus sp inoculation increases phosphorus availability in cattle manure
... improve Po hydrolysis in manure with a concomitant increase in P availability for plants requires further evaluation. The primary concerns regarding the use of these bacteria as manure inoculants are the inoculum ... See full document
11
High Prevalence of Helicobacter Species Detected in Laboratory Mouse Strains by Multiplex PCR Denaturing Gradient Gel Electrophoresis and Pyrosequencing
... the detection of murine helicobacters (12, 22, 25, ...the detection of Helicobacter ...direct detection of Helicobac- ter in animal tissues, in which colonization with more than one Helicobacter ... See full document
8
Structural Shifts of Mucosa Associated Lactobacilli and Clostridium leptum Subgroup in Patients with Ulcerative Colitis
... The mucosa-associated bacterial composition is host specific and significantly different from that of fecal bacteria (43), and the composition also is stable over a period of time (22). This suggested that the ... See full document
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