[PDF] Top 20 Evaluation of blood culture broths with lysis buffer to directly identify specific pathogens by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry methods
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Evaluation of blood culture broths with lysis buffer to directly identify specific pathogens by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry methods
... (B) lysis buffer, respectively. The mass spectra show the difference in peaks (presence or absence) and their intensities between the sample spectrum and those of bacteria identified at first pass by ... See full document
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Direct Identification of Urinary Tract Pathogens from Urine Samples, Combining Urine Screening Methods and Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... screening methods, such as cytometry and automated microscopic analysis of urine sediment, allows the rapid prediction of negative ...addition, matrix-assisted laser desorption ioniza- ... See full document
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Evaluation of a Semiquantitative Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Method for Rapid Antimicrobial Susceptibility Testing of Positive Blood Cultures
... incubation time, concentration of the substance, and the antibiotic-specific time-kill ...MALDI-TOF mass spectrometer are the same as those applied for the identification ...hands-on ... See full document
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Rapid Identification of Bacteria and Yeasts from Positive Blood Culture Bottles by Using a Lysis Filtration Method and Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrum Analysis with the SARAMIS Database
... infections directly from a positive blood culture would decrease the time to directed antimicrobial therapy and greatly improve patient ...care. Matrix-assisted laser ... See full document
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Performance of Two Resin Containing Blood Culture Media in Detection of Bloodstream Infections and in Direct Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI TOF MS) Broth Assays for Isolate Identification: Clinical Comparison of the BacT/Alert Plus and Bactec Plus Systems
... significant pathogens in simulated BCs inoculated with blood containing therapeutic levels of commonly used antibiotics and antifungal agents ...to specific microorganism-antimicrobial com- ... See full document
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Comparison of Two Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry Methods with Conventional Phenotypic Identification for Routine Identification of Bacteria to the Species Level
... requiring specific ex- traction and purification ...Molecular methods in theory should be able to identify multiple organisms at once in a single assay, with sufficient sensitivity and specificity to ... See full document
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Utility of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry following Introduction for Routine Laboratory Bacterial Identification
... Biotyper, like any identification system, has limitations even for common clinical isolates, and the identifications obtained must be interpreted by experienced laboratory personnel in conjunction with clinical ... See full document
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Identification of Blood Culture Isolates Directly from Positive Blood Cultures by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and a Commercial Extraction System: Analysis of Performance, Cost, and Turnaround Time
... Matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry represents a revolution in the rapid iden- tification of bacterial ... See full document
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Advances in Identification of Clinical Yeast Isolates by Use of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... extraction methods pro- vide a definitive identification for the majority of yeast isolates within 10 to 40 min with only 5 min of hands-on time (29, ...identification methods, which in turn may aid ... See full document
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Real Time Identification of Bacteria and Candida Species in Positive Blood Culture Broths by Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry
... bacteremia. Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF– MS) allows the identification of microorganisms ... See full document
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Usefulness of matrix-assisted laser desorption ionization time-of-flight mass spectrometry to identify pathogens, including polymicrobial samples, directly from blood culture broths
... Signal Blood Culture System (Oxoid; Thermo Fisher Scientific, Waltham, MA, USA), including both anaerobic and aerobic ...whole blood were incubated in MIR- 553 instruments (SANYO, Moriguchi, Japan) ... See full document
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Application of MALDI TOF MS to rapid identification of anaerobic bacteria
... of flight mass spectrometry (MALDI- TOF MS) has been widely used in clinical microbial diagnosis in the past ...identification methods [6, 7]. MALDI- TOF MS is a rapid mass ... See full document
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Comparing Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Phenotypic and Molecular Methods for Identification of Species within the Streptococcus anginosus Group
... test, matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS), and a PCR multiplex method to identify 212 SAG ... See full document
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Creation of an In House Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Corynebacterineae Database Overcomes Difficulties in Identification of Nocardia farcinica Clinical Isolates
... The same approach was used in our case. Reliable identifica- tion of the clinical isolate was possible only after the generation of an in-house database, expanding the stock Bruker Biotyper data- base. To our surprise, ... See full document
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Comparison of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Molecular Biology Techniques for Identification of Culicoides (Diptera: Ceratopogonidae) Biting Midges in Senegal
... However, matrix-assisted laser desorption ionizationⴚtime of flight mass spectrometry (MALDI-TOF MS) profiling is efficient for arthro- pod identification at the species ... See full document
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Implementation of Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry in Routine Clinical Laboratories Improves Identification of Coagulase Negative Staphylococci and Reveals the Pathogenic Role of Staphylococcus lugdunensis
... In 2010, MALDI-TOF MS identification was implemented with a MALDI-TOF Microflex LT/LRF coupled to the MALDI Biotyper algo- rithm, as recommended by the manufacturer (Bruker Daltonics), which replaced phenotypic ... See full document
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Discrepancy in MALDI-TOF MS identification of uncommon Gram-negative bacteria from lower respiratory secretions in patients with cystic fibrosis
... MALDI-TOF MS measurements were performed with a 60-Hz nitrogen laser and the spectra were analyzed over a mass range of 2–20 kDa. The strains from both reference and clinical sets were measured and ... See full document
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Comparison of analytical profile index, microscan walkaway 40 plus and bruker maldi biotyper systems for identification of enteric pathogens in Kenya
... TOF spectrometry are available for identification and antimicrobial susceptibility profiling of primary ...These methods are used in clinical diagnosis except MALDI TOF mass spectrometry that ... See full document
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Detection and Genotyping of SHV β Lactamase Variants by Mass Spectrometry after Base Specific Cleavage of In Vitro Generated RNA Transcripts
... post-PCR methods devised in the past, the most widely used are hybridization- based assays including fluorescence-labeled oligonucleotide probes on a LightCycler instrument (25) or DNA microarrays (12), as well as ... See full document
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Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Based Method for Discrimination between Molecular Types of Cryptococcus neoformans and Cryptococcus gattii
... A total of 107 Cryptococcus isolates, including 89 of C. neofor- mans and 18 of C. gattii, were studied. Among the clinical isolates, 56 were collected in the mycology laboratory of the Universita` degli Studi di Milano ... See full document
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