[PDF] Top 20 Evaluation of expression systems of recombinant human interferon gamma
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Evaluation of expression systems of recombinant human interferon gamma
... pastoris expression system has only recently been investigated for the commercial production of hIFNγ, reporting secretion of around 300 mg L -1 with a specific activity of 1 × 10 7 ...of human ... See full document
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Evaluation of novel inducible promoter/repressor systems for recombinant protein expression in Lactobacillus plantarum
... of expression in exponential phase, initiated by varying promoters, decreased during prolonged fermen- ...the recombinant protein ...of recombinant protein expression ...and expression ... See full document
17
Inducible Expression of Human Gamma Interferon
... Inducible expression of IFN-, IFN- Cloning, Cumate-regulatable expression cassette, Chinese hamster ovary-cumate transactivator (CHO-CTA) INTRODUCTION he premature termination of high producer clones, ... See full document
6
Expression of Interferon Gamma by a Recombinant Rabies Virus Strongly Attenuates the Pathogenicity of the Virus via Induction of Type I Interferon
... of human rabies deaths occur in children in the developing world, and it is estimated that at least 55,000 humans die of rabies each year in Africa and Asia alone ... See full document
11
Expression and Purification of Human Interferon Gamma Using a Plant Viral Vector
... an expression vector in cucurbits. We now have a tool to produce recombinant proteins at high levels making the future very promising for the role of plant viral vectors as a vehicle for industrial and ... See full document
12
Anti-Gamma Interferon Antibodies Enhance the Immunogenicity of Recombinant Adenovirus Vectors
... antigen expression in muscle following DNA-gp120 immu- nization (data not ...rAd-transgene expression in muscles that are important for immune stimula- tion cannot be detected in the experimental ... See full document
10
Organ specific cytokine therapy Local activation of mononuclear phagocytes by delivery of an aerosol of recombinant interferon gamma to the human lung
... activate human alveolar macrophages in vivo. To accomplish this, rIFN-gamma was administered to nonsmoking normals, the amounts of IFN-gamma quantified in serum and respiratory epithelial lining ... See full document
7
Evaluation of Interferon-Beta Protein Expression in HEK293T Cell-Line Transfected by Recombinant Construction, Pbud.Ifnβ-1a
... the expression level of IFNβββββ protein in HEK293T cell line transfected by recombinant construction ...of recombinant vector were examined via RFLP, Colony PCR and sequencing and finally ... See full document
6
Interferon gamma inhibits scavenger receptor expression and foam cell formation in human monocyte derived macrophages
... ScR expression in foam cells of atherosclerotic ...of interferon-gamma (IFN gamma), a lymphokine secreted by activated T lymphocytes, on the expression of ScR in human ... See full document
10
mRNA based approach to monitor recombinant gamma interferon restoration of LPS induced endotoxin tolerance
... Results: Expression analysis of those genes confirmed the presence of an immunosuppression state and the ex vivo restoration of immune functions by ...vivo evaluation of rIFN-g effect on monocyte ... See full document
11
The Antiviral Response to Gamma Interferon
... the expression of some but not all induced genes ...induced expression of IFN- and IFI56K mRNAs as indices of at least two distinct dsRNA response ...the systems involved, some cogent conclusions ... See full document
9
Counterregulatory effects of interferon gamma and endotoxin on expression of the human C4 genes
... activities. Expression of C4A and C4B genes is regulated by IFN-gamma in human hepatoma cells and in murine fibroblasts transformed with the respective ...on expression of C4A than that of ... See full document
8
Influence of recombinant bovine gamma interferon on neutrophil function
... Immune interferon enhances functional properties of human granulocytes: role of Fc receptors and effect of lymphotoxin, tumor necrosis factor, and granulocyte-macrophage colony- stimul[r] ... See full document
182
Interferon gamma is necessary for the expression of hypersensitivity pneumonitis
... increase in lung index when exposed to SR, as shown in Fig. 4. The GKO mice had a significantly higher lung index than the WT mice. There was an increase in IFN- g mRNA in WT mice exposed to antigen compared with ... See full document
6
Prostaglandin E2 selectively increases interferon gamma receptor expression on human CD8+ lymphocytes
... and interferon gamma (IFN gamma), acting sequentially, were required for the differentiation of suppressor cells in mitogen-stimulated ...IFN gamma-dependent effects on CD8+ cells by altering ... See full document
6
Mechanisms of fever induced by recombinant human interferon
... using human interferon (hIFN) derived from blood leukocytes or cell lines, fever has been a prominent component of IFN ...therapy. Human protein impurities might account for the fever to cell-derived ... See full document
9
Human Interferon-gamma (IFN-γ) Kit
... IFN-γ. Human IFN-γ is a 140 amino acids polypeptide that shows multiple effects; it induces the production of cytokines, upregulates the expression of class I and II MHC antigens, and leukocyte adhesion ... See full document
10
Posttranscriptional regulation of beta interferon expression in erythroid Friend cells treated with gamma interferon.
... 2-5A synthetase activity does not increase in interferon-resistant Friend leukemia cell variants treated with alpha/beta interferon despite the presence of high- affinity interferon rece[r] ... See full document
7
Synergistic inhibition of human cytomegalovirus replication by interferon alpha/beta and interferon gamma
... Collectively, these data suggest that the synergistic inhibi- tion of HCMV replication by IFN-α/β and IFN-γ may involve, at least in part, the regulation of IE gene expres- sion. The significance of an inhibitory block ... See full document
13
Effect of gamma interferon on expression of Fc gamma receptors in monocytes of newborn infants and adults.
... unstimulated expression of FcgRI is repre- sented by the time point 18 h in our ...RII expression immedi- ately following monocyte harvest, despite cell activation evi- denced by the increase in ...in ... See full document
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