[PDF] Top 20 Evolution of the IS6110 Based Restriction Fragment Length Polymorphism Pattern during the Transmission of Mycobacterium tuberculosis
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Evolution of the IS6110 Based Restriction Fragment Length Polymorphism Pattern during the Transmission of Mycobacterium tuberculosis
... of transmission will develop increasingly divergent pat- ...the pattern is no longer similar enough then becomes an arbitrary ...RFLP pattern, A, for which there are two similar patterns (B and C), ... See full document
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Genetic Diversity and Population Structure of Mycobacterium tuberculosis in Casablanca, a Moroccan City with High Incidence of Tuberculosis
... on tuberculosis transmission and M. tuberculosis population structure in Mo- ...M. tuberculosis (27, 40, ...and IS6110-restriction fragment length ... See full document
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Chromosomal DNA fingerprinting analysis using the insertion sequence IS6110 and the repetitive element DR as strain specific markers for epidemiological study of tuberculosis in French Polynesia
... The polymorphism of Mycobacterium tuberculosis strains was evaluated in French Polynesia, an area with a low incidence of tuberculosis and a population which has been geographically stable ... See full document
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Molecular Fingerprinting of Mycobacterium tuberculosisand Risk Factors for Tuberculosis Transmission in Paris, France, and Surrounding Area
... the tuberculosis cases reported in France are from the Ile de France ...of tuberculosis in this region is 33 cases per 100,000 inhabitants, twice the national ...A restriction fragment ... See full document
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A Combination of Two Genetic Markers Is Sufficient for Restriction Fragment Length Polymorphism Typing of Mycobacterium tuberculosis Complex in Areas with a High Incidence of Tuberculosis
... with polymorphism and their use as genetic markers for Mycobac- terium tuberculosis, strain differentiation by DNA typing has greatly facilitated epidemiological studies (7, 8, ...M. tuberculosis ... See full document
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Genetic Diversity of Mycobacterium africanum Clinical Isolates Based on IS6110 Restriction Fragment Length Polymorphism Analysis, Spoligotyping, and Variable Number of Tandem DNA Repeats
... trees based on spoligotyping and VNTR results alone and in combination were built for 30 clinical isolates that were typed by both methods (10 isolates for each of the subgroups, numbered 1 to 10 for A1, 11 to 20 ... See full document
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Stability of DNA fingerprint pattern produced with IS6110 in strains of Mycobacterium tuberculosis
... To assess the stability of IS6110 restriction fragment length polymorphism patterns, DNA fingerprints of 6 Mycobacterium bovis isolates from 1 patient and of 41 Mycobacterium tuberculosi[r] ... See full document
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Mycobacterial Interspersed Repetitive Unit Typing of Mycobacterium tuberculosis Compared to IS6110 Based Restriction Fragment Length Polymorphism Analysis for Investigation of Apparently Clustered Cases of Tuberculosis
... of polymorphism in the number of copies of tandem repeat sequences is an invaluable tool for the genotyping of higher ...M. tuberculosis and, notably, Mycobacterium bovis BCG ...M. ... See full document
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Evaluation of Semiautomated IS6110 Based Restriction Fragment Length Polymorphism Typing for Mycobacterium tuberculosis in a High Burden Setting
... M. tuberculosis genome is highly conserved compared to other bacteria, the 93% cutoff might not provide sufficient discrimination for epidemiologic ...M. tuberculosis for epidemiologic ... See full document
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Spread of Drug Resistant Pulmonary Tuberculosis in Estonia
... Restriction fragment length polymorphism (RFLP) analysis of 209 Mycobacterium tuberculosis clinical iso- lates obtained from newly detected pulmonary tuberculosis patients ... See full document
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Comparative Study of IS6110 Restriction Fragment Length Polymorphism and Variable Number Tandem Repeat Typing of Mycobacterium tuberculosis Isolates in the Netherlands, Based on a 5 Year Nationwide Survey
... only IS6110 RFLP and VNTR typing, because additional PGRS RFLP typing was used for strains with ⱕ5 copies of IS6110 (n ⫽ ...versus IS6110 RFLP ... See full document
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Restriction Fragment Length Polymorphism Analysis of Mycobacterium tuberculosis Isolated from Countries in the Western Pacific Region
... M. tuberculosis provides a versatile tool for the identification of transmission (25, 28), the investi- gation of TB outbreaks (11, 17), the distinction between reac- tivation and reinfection (26), as well ... See full document
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Epidemiologic Import of Tuberculosis Cases Whose Isolates Have Similar but Not Identical IS6110 Restriction Fragment Length Polymorphism Patterns
... the IS6110 RFLP pattern, such as the addition or loss of a fragment containing a copy of the inser- tion element or a change in the size of a fragment, occur very rarely during serial ... See full document
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Comparative Evaluation of Ligation Mediated PCR and Spoligotyping as Screening Methods for Genotyping ofMycobacterium tuberculosis Strains
... of Mycobacterium tuberculosis strains. Relying on restriction fragment length polymorphism (RFLP) analysis with IS6110 (IS6110 RFLP analysis) as a “gold standard,” ... See full document
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Comparison of Variable Number Tandem Repeat and IS6110 Restriction Fragment Length Polymorphism Analyses for Discrimination of High and Low Copy Number IS6110 Mycobacterium tuberculosis Isolates
... M. tuberculosis is IS6110 re- striction fragment length polymorphism (IS6110-RFLP) anal- ysis ...is based on the observation that RFLP patterns among non-epidemiologically ... See full document
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Evaluation of Mycobacterial Interspersed Repetitive Unit Variable Number Tandem Repeat Analysis and Spoligotyping for Genotyping of Mycobacterium bovis Isolates and a Comparison with Restriction Fragment Length Polymorphism Typing
... utilized polymorphism of the insertion sequence IS6110 and repetitive DNA elements such as the polymorphic GC-rich sequence (PGRS) and the direct-repeat (DR) ...of IS6110, the PGRS, and the DR region ... See full document
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Genome Sequence Based Fluorescent Amplified Fragment Length Polymorphism Analysis ofMycobacterium tuberculosis
... with IS6110 for typing ...of transmission not demonstrable by IS6110 ...M. tuberculosis isolates in certain significant countries like India have only one IS6110 copy ...M. ... See full document
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Stability of IS6110 Restriction Fragment Length Polymorphism Patterns of Mycobacterium tuberculosisStrains in Actual Chains of Transmission
... of IS6110 restriction fragment length polymorphism (RFLP) patterns of Mycobacterium tuber- culosis strains in actual transmission chains has been assessed by analyzing the ... See full document
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Characterization of IS6110 Restriction Fragment Length Polymorphism Patterns and Mechanisms of Antimicrobial Resistance for Multidrug Resistant Isolates of Mycobacterium tuberculosis from a Major Reference Hospital in Assiut, Egypt
... Crude lysates containing genomic DNAs for use as tem- plates for PCR were prepared from Middlebrook 7H9 broth cultures of bacterial isolates by disruption of cells with sili- conized glass beads as previously described ... See full document
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Genetic Heterogeneity in Mycobacterium tuberculosisIsolates Reflected in IS6110 Restriction Fragment Length Polymorphism Patterns as Low Intensity Bands
... M. tuberculosis complex isolates analyzed from May 1997 to June 1998 in The Netherlands were screened for indications of mul- tiple infections expressed by the presence of low-intensity background ...1,277 ... See full document
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