[PDF] Top 20 Expression in animal cells and characterization of the hepatitis E virus structural proteins.
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Expression in animal cells and characterization of the hepatitis E virus structural proteins.
... mal cells, however, a cellular protein, which we have called 3IP, was found to interact with ...hepatoma cells, not with pORF3 from HepG2 hepatoblastoma ... See full document
10
Evaluation of a Recombinant Measles Virus as the Expression Vector of Hepatitis C Virus Envelope Proteins
... E2 proteins were detected in rMV-E1E2-, rMV-Ed- and mock-infected B95a cells by western blot with MoAb 384 for E1 and MoAb 384 and 544 for E1 and E2 ... See full document
6
Expression, characterization, and immunoreactivities of a soluble hepatitis E virus putative capsid protein species expressed in insect cells.
... unique expression of this coding se- quence outside the context of the full-length protein would produce a protein with the same desirable physical and immu- nogenic characteristics shown by the p62-kDa ...lovirus ... See full document
6
Identification of genotype 4 Hepatitis E virus binding proteins on swine liver cells
... Hepatitis E virus (HEV), a member of the genus Hepe- virus, is a non-enveloped virus with a positive-stranded RNA genome approximately ...non-structural proteins, ... See full document
6
Hepatitis C Virus Nonstructural Protein 5A: Biochemical Characterization of a Novel Structural Class of RNA-Binding Proteins
... Hepatitis C virus (HCV) nonstructural protein 5A (NS5A) exhibits a preference for G/U-rich RNA in vitro. Biological analysis of the NS5A RNA-binding activity and its target sites in the genome will be ... See full document
12
Characterization of the Polyproline Region of the Hepatitis E Virus in Immunocompromised Patients
... of virus protein to the viroplasm ...the virus life cycle ...treated cells partially reverses the inhibitor effect on HEV replica- tion ...of virus replication must still be demonstrated, ... See full document
9
Expression and processing of the Hepatitis E virus ORF1 nonstructural polyprotein
... the virus was replaced with Excel 405 ...infected cells were har- vested at 24, 48, 60 and 72 hours post-infection ...total proteins were separated by SDS-polyacrylamide gel electrophoresis (PAGE) ... See full document
9
Expression and self-assembly of empty virus-like particles of hepatitis E virus.
... and expression of capsid pro- teins. Sf9 cells (Riken Cell Bank, Tsukuba, Japan) derived from an insect, Spodoptera frugiperda (27), were cotransfected with linearized wild-type Au- tographa californica ... See full document
7
Subcellular Localization of Hepatitis C Virus Structural Proteins in a Cell Culture System That Efficiently Replicates the Virus
... heterologous expression systems or in the context of a full-length HCV replicon (1, 22, ...infected cells, its localization at the potential site of lipid drop- let formation suggests that the C protein ... See full document
10
Hepatitis C Virus NS2 Protein Serves as a Scaffold for Virus Assembly by Interacting with both Structural and Nonstructural Proteins
... infectious virus production (data not shown) (12). Thus, the dicistronic expression of HJ3-5 polyprotein per se is not responsible for the failure of the HJ3-5/E2 HA /p7 YFP /IRES RNA to produce ...restore ... See full document
12
Over-expression and characterization of NS3 and NS5A of Hepatitis C virus genotype 3a
... Background: Hepatitis C virus (HCV) is a common and leading cause for liver cirrhosis and hepatocellular carcinoma. Current therapies to treat HCV infection are shown to be partially effective and poorly ... See full document
12
Yellow Fever Virus Replicons as an Expression System for Hepatitis C Virus Structural Proteins
... during virus assembly with the cytoplasmic domains of the YF, or possibly HCV, prebudding ...of E (YF nt 482 through 2318) were replaced by ...the structural gene region of ... See full document
5
Hepatitis C Virus Structural Proteins Assemble into Viruslike Particles in Insect Cells
... C virus (HCV) is a leading cause of chronic hepatitis in the ...the virus in cultured cells, and the lack of a convenient animal ...the virus nor the prerequisites for its ... See full document
10
Rift Valley fever virus structural proteins: expression, characterization and assembly of recombinant proteins
... enveloped virus with a diameter of 90 to 110 nm and a core element of 80 to 85 nm ...the virus particles [9]. The S segment codes for two proteins, the structural nucleo- protein (N) in the ... See full document
13
Expression and characterization of virus-like particles containing rubella virus structural proteins.
... ELISA was used to quantify the antibody titers against each of the RV structural proteins, by using individual purified RV structural proteins expressed in SF9 cells infected with baculo[r] ... See full document
6
Characterization of Hepatitis E Virus Recombinant ORF2 Proteins Expressed by Vaccinia Viruses
... experiment, cells were fixed in 4% paraformaldehyde (PFA) or in cold absolute ...PFA-fixed cells were permeabilized before processing (27). All cells were further incu- bated with phosphate-buffered ... See full document
7
Expression of processed core protein of hepatitis C virus in mammalian cells.
... The assay detecting antibody to HCV using a molecularly expressed nonstructural HCV protein, C100, has been applied for blood screening and diagnosis of chronic hepatitis 7, 12, 16.. How[r] ... See full document
7
Suppression of hepatitis B virus expression and replication by hepatitis C virus core protein in HuH-7 cells.
... To elucidate the molecular status of superinfection with these two hepatitis viruses, we cotransfected the full-length or truncated version of HCV structural genes core and envelope 1 to[r] ... See full document
10
Structural characterization of virion proteins and genomic RNA of human parainfluenza virus 3.
... The M protein of HPIV3 has a molecular weight of 35 x 1 is associated with the envelope of the virion, and aggregates under low-salt conditions, and intrachain disulfide bonds affect its[r] ... See full document
6
Genetic Characterization and Sequence Heterogeneity of a Canadian Isolate of Swine Hepatitis E Virus
... the sequences of the individual ORFs of the Arkell virus with the respective sequences of the ORFs of other swine HEVs, and very similar phylogenetic observations have been obtained (Fig. 3B and C). Partial ... See full document
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