[PDF] Top 20 Expression, purification, and properties of recombinant encephalomyocarditis virus RNA-dependent RNA polymerase.
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Expression, purification, and properties of recombinant encephalomyocarditis virus RNA-dependent RNA polymerase.
... Lane 4, purified recombinant EMC virus 3DP01 obtained by thrombin cleavage of the fusion protein while immobilized on glutathione.. The positions of the molecular mass markers in kilodal[r] ... See full document
8
Identification and Characterization of the Escherichia coli-Expressed RNA-Dependent RNA Polymerase of Bamboo Mosaic Virus
... and purification of the products of ...the RNA genome, which is a critical step in the pathogenesis of many RNA ...chemical properties of RdRp comes from studies of animal RNA viruses, ... See full document
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Characterization of Soluble Hepatitis C Virus RNA-Dependent RNA Polymerase Expressed in Escherichia coli
... Parallel expression and purifi- cation of full-length and truncated NS5B from HCV-1b, the BK ...for expression driven by T7 ...The purification procedures were sim- ilar to those previously reported ... See full document
6
Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps
... Inefficient expression of genes from RNA viruses by RNA polymerase II (Pol II) dependent cellular promoters might be explained by lack of critical elements required for pre-mRNA ... See full document
10
Generation and characterization of a recombinant Rift Valley fever virus expressing a V5 epitope tagged RNA dependent RNA polymerase
... L3V5 polymerase showed 24 % of the activity of wt in the minigenome ...reporter-gene expression in an analogous minigenome ...viral RNA synthesis in a minigenome system (Blakqori et ...T7 ... See full document
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De Novo Initiation of RNA Synthesis by the RNA-Dependent RNA Polymerase (NS5B) of Hepatitis C Virus
... homopolymer RNA templates in vitro (7, 17, 35–38, ...intrinsic properties of HCV NS5B, cDNA of NS5B was directly amplified from HCV viral RNA extracted from an HCV-infected patient’s serum and used ... See full document
13
Alfalfa Mosaic Virus Coat Protein Bridges Rna and Rna-dependent Rna Polymerase in Vitro
... Expression, purification, and activity of expressed AMV P1 and P2 ...proteins. Recombinant baculoviruses with the 6His-tagged P1 (helicase-methyltransferase) or P2 (RNA-dependent ... See full document
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A Recombinant Hepatitis C Virus RNA-Dependent RNA Polymerase Capable of Copying the Full-Length Viral RNA
... reported recombinant RNA-dependent RNA polymerases (RdRp), the NS5B enzymes, of hepatitis C virus (HCV) could function only in a primer-dependent and template-nonspecific manner, ... See full document
9
Influenza A virus RNA-dependent RNA polymerase: analysis of RNA synthesis in vitro.
... NP expression and purification. Monolayer cultures of Sf9 cells were infected with an NP-baculovirus recombinant (8) at a multiplicity of infection of 5 PFU per cell. Subsequently, infected cultures ... See full document
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Template Requirements for RNA Synthesis by a Recombinant Hepatitis C Virus RNA-Dependent RNA Polymerase
... T7 RNA polymerase used was purified by the protocol of Grodberg and Dunn ...the RNA was eluted from the polyacrylamide with ...the RNA concentration was determined by spectro- photometry and ... See full document
8
Mutational Analysis of Bovine Viral Diarrhea Virus RNA-Dependent RNA Polymerase
... diarrhea virus (BVDV) nonstructural protein 5B (NS5B) produced in insect cells has been shown to possess an RNA-dependent RNA polymerase (RdRp) ...the expression of insoluble ... See full document
8
Norovirus Proteinase-Polymerase and Polymerase Are Both Active Forms of RNA-Dependent RNA Polymerase
... the virus growth inhibition experiments, CRFK cells were preincubated for 1 h at 37°C, in the presence of 2 mM nucleoside analogs or ribavirin in virus growth medium (VM) consisting of Eagle’s minimum ... See full document
11
De Novo Initiation of RNA Synthesis by the Arterivirus RNA-Dependent RNA Polymerase
... initiated RNA synthesis de novo on homopolymeric templates in a template-specific ...novo RNA synthesis generally initiates with a purine as the starting ...of RNA synthesis or runoff polymerization ... See full document
12
Selection of RNA Aptamers That Are Specific and High-Affinity Ligands of the Hepatitis C Virus RNA-Dependent RNA Polymerase
... NS5B polymerase by selected ...the RNA-protein interaction, gel mobility retardation and filter-binding assays were ...labeled RNA aptamers ... See full document
9
RNA duplex unwinding activity of poliovirus RNA-dependent RNA polymerase 3Dpol.
... Belozersky Institute of Physical Chemical Biology, Moscow State University, Moscow 119899,2 and Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences, [r] ... See full document
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Purification and properties of a bacteriophage T5-modified form of Escherichia coli RNA polymerase.
... We compared the in vitro transcriptional properties of RNAPT515 with those of three other purified enzyme preparations: i RNA polymerase from uninfected non-colicinogenic cells; ii RNA p[r] ... See full document
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Structural and Functional Characterization of Sapovirus RNA-Dependent RNA Polymerase
... multiparameter expression screening in Escherichia coli was performed using a sparse matrix (1), followed by a dot blot procedure ...best expression condition [Rosetta2 (DE3)pLysS (Novagen) strain using 2YT ... See full document
14
Biochemical and Genetic Studies of the Initiation of Human Rhinovirus 2 RNA Replication: Purification and Enzymatic Analysis of the RNA-Dependent RNA Polymerase 3Dpol
... picornavirus RNA replication is a two- step process carried out primarily by the viral RNA polymerase in conjunction with other viral and possibly also cellular pro- teins (reviewed in references 1 ... See full document
10
Characterization of an RNA-dependent RNA polymerase activity associated with La France isometric virus.
... Analysis of LIV-enriched precipitates. Precipitates (3,000 3 g) derived from clarified homogenates of diseased mushrooms that had been incubated for 4 weeks at 48C (method I) were highly enriched in LIV. The ... See full document
6
Conformational Flexibility of the Dengue Virus RNA-Dependent RNA Polymerase Revealed by a Complex with an Inhibitor
... formational changes in the protein (Fig. 3D). Binding of the com- pound stabilizes an extra turn of helix ␣ 7, and loop 3, which is disordered in the free protein, becomes ordered upon the forma- tion of several ... See full document
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