[PDF] Top 20 Fast detection of Noroviruses using a real time PCR assay and automated sample preparation
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Fast detection of Noroviruses using a real time PCR assay and automated sample preparation
... strategies Real-time PCR affords continuous monitoring of the PCR reaction cycle by cycle by using ...our assay is based on the binding of the fluorescent dye SYBR Green, which ... See full document
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Characteristics of the m2000 Automated Sample Preparation and Multiplex Real Time PCR System for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae
... m2000 assay in clinical trials, we elected to perform a preliminary assessment of assay performance by testing residual material from swab and urine samples previously collected for and tested by other FDA- ... See full document
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Rapid Quantification of Hepatitis B Virus DNA by Automated Sample Preparation and Real Time PCR
... the detection and quantification of HBV DNA have been ...lack automated sample ...on PCR, are limited by a short dynamic range (2 to 3 log ...LC PCR Reagents in conjunction with ... See full document
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Development of a real time RT PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland
... separate Real-time RT-PCR assays were designed based on SYBR green chemistry for GI and GII ...This assay utilised a fluorescence acquisition step at 85°C for GI and 84°C for GII to melt ... See full document
8
Detection of ovine milk adulteration using taqman real-time pcr assay
... Cheese Assay, cattle and sheep species were identified by using specific primers complementary to the cattle and sheep ...reaction. PCR amplification was performed according to the manufacturer´s ... See full document
6
Novel real-time PCR detection assay for Brucella suis
... out using the HighPure PCR Template Preparation Kit (Roche Diagnostics, Mannheim, Germany) according to the man- ufacturer ’ s instructions for ...determined using a NanoDrop ™ 1000 spectro- ... See full document
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Real-time PCR quantitation of hepatitis B virus DNA using automated sample preparation and. murine cytomegalovirus internal control
... novel real-time PCR assay described in this paper offers several important advantages for HBV DNA ...95% detection limit of 24.4 IU/ml, ii) very high intra-assay and ... See full document
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Real-time PCR solutions from sample preparation to data analysis
... OVERVIEW Real-time PCR Real-time PCR solutions from sample preparation to data analysis For over a quarter century, we’ve provided you with reliable reagents, ... See full document
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Development and validation of a real time PCR assay for the detection of clinical acanthamoebae
... designed assay was sensitive enough to detect DNA from all rep- resented species sent to our reference ...each sample using three different ...point PCR primers made specifically for ... See full document
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Development of a Quantitative Real Time PCR Assay for Detection of Mycoplasma genitalium
... sensitive PCR methods are needed for diagnosis of this bacterium because it is very difficult to culture from patient ...quantitative real-time LightCycler PCR was ...The assay could ... See full document
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Development and Evaluation of a Real Time PCR Assay for Detection of Pneumocystis jirovecii on the Fully Automated BD MAX Platform
... this PCR and to make it available for use apart from the normal working hours, we thought to mi- grate this assay onto the fully automated BD MAX platform run in the open ...The PCR reagents ... See full document
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Comparison of a Multiplex Real Time PCR Assay with a Multiplex Luminex Assay for Influenza Virus Detection
... LC using the total nucleic acid extraction kit (Roche Diag- nostics, Indianapolis, ...The sample and elution volumes were 200 ...by real-time RT-PCR the same ... See full document
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Comparison of commercial DNA preparation kits for the detection of Brucellae in tissue using quantitative real time PCR
... rapid detection using real-time or conventional PCR ...techniques. Real-time PCR assays are rapid, specific and have a very low limit of detection, when ... See full document
5
Automated Extraction and Quantification of Human Cytomegalovirus DNA in Whole Blood by Real Time PCR Assay
... In summary, we have shown that a high-throughput auto- mated system that has three easy steps has considerable clinical potential. The three key points are as follows: the use of WB instead of separated, counted PBL; ... See full document
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Development and evaluation of a real time PCR assay for the quantitative detection of Theileria annulata in cattle
... on PCR and hybridisation assays (RLB or Luminex W ) are available for the detection and identification of ...a real-time PCR assay that can be used for both the detection ... See full document
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Multiplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis.
... Real-time PCR. Multiplex TaqMan real-time PCR was performed in the format of two duplex reactions using ABI 7500 system (Applied Biosystems) in a volume of 25 µl in an ... See full document
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A TaqMan real time PCR assay for the detection and quantitation of Plasmodium knowlesi
... Results Assay design A real-time PCR assay for ...screening assay published by Rougemont et al for ...Their assay enables rapid screening of patient specimens for Plasmo- ... See full document
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A novel real time PCR assay for specific detection of Brucella melitensis
... general real-time PCR amplifying DNA of all Brucella strains, as well as by Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spec- trometry (MALDI-TOF MS) [22, ... See full document
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Rapid Preclinical Detection of Sheeppox Virus by a Real-Time PCR Assay
... CaPV real-time PCR assay provides a rapid, sensitive test for CaPV that is able to detect SPPV prior to the onset of clinical ...preclinical detection of SPPV, the sample of ... See full document
7
Rapid Preclinical Detection of Sheeppox Virus by a Real Time PCR Assay
... CaPV real-time PCR assay provides a rapid, sensitive test for CaPV that is able to detect SPPV prior to the onset of clinical ...preclinical detection of SPPV, the sample of ... See full document
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