[PDF] Top 20 The Functional Characterization of the N-terminal Domains of TUT4
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The Functional Characterization of the N-terminal Domains of TUT4
... Scientific). TUT4-N and pGEX-6p2 vector were ligated in a 3:1 ratio using T4 DNA ligase (New England Biolabs #M0202L) at 37°C for 1 hour, followed by heat-shock transformation into TOP10 competent ... See full document
136
Genetic and Functional Characterization of the N-Terminal Region of the Hepatitis C Virus NS2 Protein
... [39]). For most mutants, infectious titers in Huh-7.5.1 cells were increased approximately 0.5 to 1 log except for a small group (G25A, A67L, K81A, W82A, and A85L) that exhibited no or mod- est changes. Interestingly, ... See full document
16
Functional Analysis of Adenovirus Protein IX Identifies Domains Involved in Capsid Stability, Transcriptional Activity, and Nuclear Reorganization
... the N-terminal ...the functional significance of these domains, the effects of a series of deletions or point mutations altering the conserved sequence elements were examined ...the ... See full document
11
Characterization of the functional properties of the neuroectoderm in mouse Cripto / embryos showing severe gastrulation defects
... refine the antero-posterior (A-P) specification of the three main domains in the brain primordium: the forebrain, the midbrain and the hindbrain. These secondary organizers are the anterior neural ridge (ANR) at ... See full document
10
Functional analysis of the Xenopus frizzled 7 protein domains using chimeric receptors
... transmit the signal. The result of such an interac- tion would be the loss of function of this Wnt protein. Because NT7-C5 chimeric receptor con- tains the ligand-binding domain of Xfz7 and there- fore, can bind to the ... See full document
6
Cloning and functional characterization of the intersex homologous gene in the pest lepidopteron Maruca vitrata
... In summary, while we have no idea whether also in B.mori as in M.vitrata is present a sex specific transcript in pupae, our data suggest that, differently from Drosophila, in these two Lepidoptera, ix pre-mRNA is subject ... See full document
7
Crystal Structure of the Oligomerization Domain of the Phosphoprotein of Vesicular Stomatitis Virus
... The N-terminal and C-terminal domains of SeV P bind N, while the central domain (residues 320 to 446) is responsible for oligomerization of a tetramer, as shown in the crystal struc- ... See full document
7
A structural constraint for functional interaction between N terminal and C terminal domains in simian immunodeficiency virus capsid proteins
... cytotoxic T lymphocytes (CTLs) were previously shown to respond to SIVmac239 but not SIVsmE543-3 infection. A mutant SIVmac239, SIVmac239Gag205E, whose Gag205D is replaced with Gag205E showed lower replicative ability. ... See full document
10
Functional characterization of the N-terminal and C-terminal domains of a sesame group II phytocystatin
... For over-expression of non-fusion SiCYS in E. coli, the recombinant plasmid pET-28a containing SiCYS was ob- tained from our previous work (Shyu et al. 2004). In this study, two sets of primers with the restriction ... See full document
10
Functional Analysis of N-Terminal Residues of Ty1 Integrase
... other domains of IN suggest that the hydrophobic residues function in IN-IN interaction, while the histidine/cysteine res- idues profoundly affect one or more early steps in retrotrans- position, resulting in a ... See full document
10
Managing the Functional Obsolescence of Machinery and Equipment: the Quotient of Curability of Functional Obsolescence as a Basis for Decisions Made about the Renovation or Replacement of Machinery and Equipment
... of functional obsolescence is technically correctible, but the question also arises whether all corrections are ...the functional obsolescence (equation 2), and the investment (I), needed for its ...of ... See full document
8
Structure function analysis reveals that the Pseudomonas aeruginosa Tps4 two partner secretion system is involved in CupB5 translocation
... We first generated mutations in the conserved TPS motifs of CupB5. We introduced the double point mutation N85A/P86A, part of the conserved NPNG box, which was designed to disrupt interactions with the POTRA1 domain. We ... See full document
19
The role of the N-terminus of the large subunit of ribulose-bisphosphate carboxylase investigated by construction and expression of chimaeric genes.
... coli, effectively replacing the 12 amino-terminal residues of the Anacystis large subunit with the 15 amino-terminal residues from wheat or maize (Fig. 3), the properties [r] ... See full document
8
The role of plasma N-terminal brain natriuretic pro-peptide in diagnosing elderly patients with acute exacerbation of COPD concurrent with left heart failure
... Methods and results: LHF was diagnosed in patients with AECOPD according to echocardio- graphic criteria, and the levels of NT-proBNP in plasma were measured by quantitative electro- chemiluminescence assay. Among the ... See full document
10
Functional role of the nicotinic arm of the acetylcholine regulatory axis in human B-cell lines
... Abstract: We studied the involvement of nicotinic acetylcholine receptors (nAChRs) in the inflammation-related activity of human B-cell lines. Activation of nAChRs in Daudi cells with epibatidine abolished the ... See full document
7
Analysis of the Assessment of Functional Office Credit Number of Nurses in Hospitals
... Abstract:-The functional position of nurses is an important element for nurses in carrying out the duties, responsibilities, and authority because it happens so as not to overlap in the discharge of his duty, ... See full document
6
The N Terminus and C Terminus of Herpes Simplex Virus 1 ICP4 Cooperate To Activate Viral Gene Expression
... Recombinant plasmids. The plasmids pK1-2, pn7, d3-8, nd3-8, d8- 10, and nd8-10 have been previously described (10, 46). pK1-2 and pn7 encode the promoter and gene for wild-type (wt) (strain KOS) and n208 ICP4, ... See full document
13
The Influence of type of Functional Groups on the Adsorption Selectivity of Ionic Imprinted Polymer (Iip) Fe
... In the synthesis of IIP, each functional polymer was contacted with 50 ppm Fe (III) ion (pH 3) and stirred for 24 hours. Then the result was filtered using filter paper and dried. Fe-functional plymer ... See full document
6
DNA Helicase Activity Is Associated with the Replication Initiator Protein Rep of Tomato Yellow Leaf Curl Geminivirus
... the functional cellular homologs of the viral SF3 ...binding domains, and the latter is absent in Rep122-359; consequently, improper interactions may occur, leading to the formation of anomalous ... See full document
9
The identification of protein domains that mediate functional interactions between Rab-GTPases and RabGAPs using 3D protein modeling
... protein domains common to Rab proteins, which they classified as the Rab family motif and Rab-family sub- ...these domains among Rabs has made ascribing a functional role to them ...these ... See full document
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