[PDF] Top 20 Generation of bovine transgenics using somatic cell nuclear transfer
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Generation of bovine transgenics using somatic cell nuclear transfer
... of cell culture to propagate donor cells can lead to almost limitless number of transgenic cells that can be frozen and stored for long periods of ...every cell of an animal will have the transgene, thereby ... See full document
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Generation of GHR modified pigs as Laron syndrome models via a dual sgRNAs/Cas9 system and somatic cell nuclear transfer
... GHRKO cell lines were established by single cell cloning ...knockout cell lines were selected as the donor cell line for somatic cell nuclear transfer for the ... See full document
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Generation of an MC3R knock-out pig by CRSPR/Cas9 combined with somatic cell nuclear transfer (SCNT) technology
... To generate MC3R knock-out piglets, biallelically modified colonies and wild-type control colonies were used simultaneously as donor cells for somatic cell nu- clear transfer (SCNT). A total of 1203 ... See full document
8
Efficient generation of P53 biallelic knockout Diannan miniature pigs via TALENs and somatic cell nuclear transfer
... tumors [12, 43, 44]. P53-mutant mice are usually used to investigate Li-Fraumeni syndrome, which may cause a variety of tumors, including sarcomas, breast cancers, brain tumors and adrenocortical carcinomas [45]. How- ... See full document
11
Richter, Anne (2012): Genetic modification of primary cells for the generation of transgenic pig models using somatic cell nuclear transfer. Dissertation, LMU München: Tierärztliche Fakultät
... from cell stress during ...on cell number, growth state and their state of differentiation, because those factors influences the total net ...kidney cell population consists of various cell ... See full document
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Efficient generation of GGTA1-null Diannan miniature pigs using TALENs combined with somatic cell nuclear transfer
... By using limiting dilution method for the GTKO cell colonies’ selection, we successfully obtained three GGTA1 biallelic knockout colonies and produced the cloned piglets with the expected genotype from one ... See full document
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SV40 Large T Antigen Disrupts Embryogenesis of Canine and Porcine Somatic Cell Nuclear Transfer Embryo
... Lentiviral Vector Construction and Lentivirus Production To prepare the lentiviral vector pLL-CMV-SV40LT-blast, the SV40LT gene was digested with two restriction en- zymes, SalI (blunted by Klenow fragment) and SnaB1 ... See full document
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Synthetic epigenetics—towards intelligent control of epigenetic states and cell identity
... the nuclear transfer and induced pluripotency experi- ments when compared to epigenetic ...with somatic cell nuclear transfer and during generation of induced pluripotent ... See full document
12
Progress toward generating a ferret model of cystic fibrosis by somatic cell nuclear transfer
... 1000 cell clones. We settled on using a 96-well plate to screen crude cell lysates by ...of cell clones, and will thus likely increase cellular compe- tence for NT ...other cell type ... See full document
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Generation of Cashmere Goats Carrying an EDAR Gene Mutant Using CRISPR-Cas9-Mediated Genome Editing
... and somatic cell nuclear transfer (SCNT) were used to obtain Cashmere goats with an EDAR gene mutation, providing an animal model and experimental material for studying the relationship ... See full document
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Recombinant human activin A promotes development of bovine somatic cell nuclear transfer embryos matured in vitro
... cultured bovine embryos (Camous et ...of bovine SCNT at the previous 8-cell stage, yet, from the 8-cell stage onwards the exogenous addition of activin A to the culture medium is es- ...and ... See full document
9
The influence of interspecies somatic cell nuclear transfer on epigenetic enzymes transcription in early embryos
... out using SuperScript First-Strand Synthesis kit (Invitrogen, Carlsbad, USA) in final volume of 20 μl using ...for bovine and porcine DNMT1 and DNMT3a genes were designed for detection of de novo ... See full document
9
Development of genome engineering technologies in cattle: from random to specific
... embryo transfer into a recipient was performed because of the ease of the ...cattle, somatic cell nuclear transfer (SCNT) was introduced and successfully used to produce cloned ...in ... See full document
9
Cellular reprogramming in farm animals: an overview of iPSC generation in the mammalian farm animal species
... Reprogramming somatic cells by SCNT is technically challenging, laborious, inefficient (especially in human), and requires the use of .../ nuclear transfer ex- periments showed that oocytes obviously ... See full document
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Cloning animals by somatic cell nuclear transfer – biological factors
... treated bovine fetal fibroblast cells and mouse stem cells with much higher doses of 5-aza-C (1 or 5 µm) and also found that blastocyst development of cloned embryos were ... See full document
7
Somatic cell nuclear transfer: failures, successes and the challenges ahead
... inner cell mass and trophectoderm in cloned mice blastocysts (Nolen et ...2005). Using Xist-deleted donor cells, to prevent this ectopic Xist expression, improved full- term development more than 7-8 times ... See full document
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Interspecies somatic cell nuclear transfer is dependent on compatible mitochondrial DNA and reprogramming factors
... donor cell and the recipient ...interspecific bovine [43] and porcine [24] SCNT offspring where the sequence variants between the donor cell and recipient oocyte mtDNA populations result in different ... See full document
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Molecular conditions of the cell nucleus remodelling/reprogramming process and nuclear transferred embryo development in the intraooplasmic karyoplast injection technique: a review
... donor cell plasma membrane in the cytoplasmic microenvironment of the enucleated oocyte is ...of nuclear donor cell or its specific surface antigen proteins as belonging to a foreign (allogeneic) ... See full document
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The Influence of Genetic Background on Stem Cell Derivation and Variability.
... increased cell viability in ESCs [123,124], but both have reduced expression in ...G1 cell cycle phase and a high proportion of cells in S phase [reviewed in ... See full document
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Sip1 regulates the generation of the inner nuclear layer retinal cell lineages in mammals
... ganglion cell layer (GCL). These different classes of retinal cell types are generated in vertebrates in a partly overlapping, yet stereotypic pattern from a pool of proliferating retinal progenitor cells ... See full document
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