[PDF] Top 20 Handedness of the Herpes Simplex Virus Capsid and Procapsid

... of herpes simplex virus type 1 (HSV-1) (19) and equine herpes virus 1 (2) and now also covering cytomegalovirus, both the human (6) and simian (22) strains; Kaposi’s sarcoma-associated ... See full document

... mature capsid-specific VP5 epitope 8F5 (5, 11, ...mature capsid-specific epitopes fail to form under condi- tions of ATP depletion and have hypothesized that this reflects an ATP dependence in VP26 ... See full document

... molecules. Procapsid assembly would involve a nucleation step in which a portal binds to one end of the scaffold string, followed by extension steps involving major capsid protein molecules bound at ... See full document

... of procapsid assembly and are assem- bled into a unique vertex (9, ...the procapsid by interacting either with coat proteins or with the growing procapsid shell (7, ...All capsid vertices (12 ... See full document

... HSV-1 capsid requires an internal scaffold- ing protein called ...major capsid protein inter- acts with 25 amino acids in the carboxy-terminal domain of pre-VP22a; these residues are cleaved upon release of ... See full document

... progeny procapsid by way of the portal or connector, a ring-shaped structure located at a unique site in the procapsid ...of capsid formation are sim- ilar in the two and because HSV-1 encodes a ... See full document

... from procapsid to C capsid during the encapsida- tion reaction and as they further develop into extracellular virions (34, 54, ...surrounding capsid or indirectly through maintaining structural ... See full document

... Organization of viral dsDNA. The radial density plot shown in Fig. 2 revealed that the density inside the capsid shell of the virion, which is contributed by the viral DNA, appears as a regular pattern of peaks ... See full document

... Capsid labeling with UL25-specific antibody. Immunoelectron microscopy was performed with capsids adsorbed to carbon-Formvar-coated copper grids. The procedure used for labeling was the same as that described ... See full document

... During construction and isolation of PrV mutants deficient in synthesis of glycoprotein gll homolog of HSV-1 gB, we observed that we were unable to isolate on gII-expressing complementin[r] ... See full document

... all herpes- viruses ...from virus-infected cells and found to be a capsid-associated polypeptide which pos- sessed both exonucleolytic and endonucleolytic ... See full document

... In contrast, the TrpE-UL35 antiserum reacted with two proteins, designated pl2a and pl2b, which were present in lysates of HSV-infected cells radiolabeled with either [35S]methionine or [r] ... See full document

... the capsid when it fuses with the cell membrane during ...the capsid upon entry and during transit to the nucleus (18, 19, 32, 33, ...the capsid as loosely associated tegument and tegument that re- ... See full document

... Phenotypic analysis of mlOO shows that i normal amounts of the c and d forms of ICP35 were produced, but they failed to be processed to the cleaved forms, e and f; ii viral DNA replicati[r] ... See full document

... the capsid, which has a defined structure, the tegument and envelope are highly variable among HSV-1 ...the capsid but failed to show the bulk of the tegument and envelope (28), and by tomographic ... See full document

... The results shown in Fig. 2A suggest that less pU L 17 was present in clarified lysates of cells infected with viruses lacking U L 19 or U L 18. To determine whether this reflected lower levels of pU L 17 in cells ... See full document

... Because it is possible that the coimmunoprecipitation and affinity copurification of pU L 17 and VP13/14 were consequen- tial to immunoprecipitation or purification of intact capsids with which both proteins might ... See full document

... of capsid decoration by conformational changes of ...HSV-1 capsid, six monomers of the small accessory protein VP26 (12 kDa) bind around the outer rim of each hexamer of UL19, the major capsid ... See full document

... dual-fluorescent-protein-tagged virus. Previ- ously, K26GFP, a recombinant HSV-1 virus in which the eGFP ORF was fused at the N terminus of VP26 (7), the small capsid decoration protein, was ... See full document

... Electron nicroscopic analysis of extracted capsids revealed that the pentons and the material found inside the cavity of B capsids primarily VP22a were removed nearly quantitatively, but[r] ... See full document