[PDF] Top 20 Hepadnavirus envelope proteins regulate covalently closed circular DNA amplification.
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Hepadnavirus envelope proteins regulate covalently closed circular DNA amplification.
... We have previously shown that within the first 4 days after infection of primary duck hepatocytes by the duck hepatitis B virus DHBV, the amount of cccDNA in the infected cells increased[r] ... See full document
6
Characterization of the Intracellular Deproteinized Relaxed Circular DNA of Hepatitis B Virus: an Intermediate of Covalently Closed Circular DNA Formation
... a DNA fragment containing HBV nt 2331 to 3182 and 1 to 1990 (GenBank accession number V01460) was retrieved from pCMVHBV (where CMV is cytomegalovirus) (6) by BspEI digestion and blunted with Kle- now enzyme, ... See full document
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The covalently closed duplex form of the hepadnavirus genome exists in situ as a heterogeneous population of viral minichromosomes.
... of covalently closed circular (CCC) DNA molecules in the nucleus of the infected ...sole DNA template for the synthesis by RNA polymerase II of all viral transcripts throughout the ... See full document
8
Roles of the Envelope Proteins in the Amplification of Covalently Closed Circular DNA and Completion of Synthesis of the Plus-Strand DNA in Hepatitis B Virus
... In this study, cccDNA synthesis is studied in the context of a proliferating cell line, Huh7-H1. These cultures are different from nondividing hepatocytes of the liver, which the virus would encounter initially upon ... See full document
12
Zinc Finger Proteins Designed To Specifically Target Duck Hepatitis B Virus Covalently Closed Circular DNA Inhibit Viral Transcription in Tissue Culture
... amplify each ZFP by PCR. ZFPa was amplified with the primers ZFPa.fw (GGATCCATGC ATCATCACCA TCACCATCCC AAGAAAAAGC GTAA GGTCCT CGAACCCGGC GAAAAGCCTT AT) and ZFPa.rv (GAATTC ACTT GTCTTCTTAC CTGTGTGG), ZFPb with ZFPb.fw ... See full document
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Half-Life of the Duck Hepatitis B Virus Covalently Closed Circular DNA Pool In Vivo following Inhibition of Viral Replication
... studying hepadnavirus biology. These viruses are characterized by small, circular, partially double- stranded DNA ...viral DNA is covalently linked to protein at its 5⬘ end and contains ... See full document
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New perspectives on the hepatitis B virus life cycle in the human liver
... HBV DNA (covalently closed circular DNA [cccDNA] and pro- tein-free relaxed circular DNA [rcDNA]), RNA, and protein in the same liver sec- ...HBV DNA and RNA and ... See full document
5
Alteration of Mature Nucleocapsid and Enhancement of Covalently Closed Circular DNA Formation by Hepatitis B Virus Core Mutants Defective in Complete-Virion Formation
... relaxed circular (RC) DNA as a result of reverse ...viral envelope proteins, leading to secretion extracellularly as virions, or (ii) disassembly (uncoating) to deliver their RC DNA ... See full document
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Age-Related Differences in Amplification of Covalently Closed Circular DNA at Early Times after Duck Hepatitis B Virus Infection of Ducks
... small DNA viruses that cause both tran- sient and persistent infections of the liver (2, ...of hepadnavirus infection be- gins with the formation of covalently closed circular ... See full document
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Formation of Hepatitis B Virus Covalently Closed Circular DNA: Removal of Genome-Linked Protein
... PF-RC DNA than CCC DNA in the HepG-2 cells, DHBV CCC DNA was always the predominant PF DNA spe- cies in vivo and in LMH ...DHBV DNA into the HEK293 cells and again found that, in these ... See full document
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n-Butyrate, a cell cycle blocker, inhibits early amplification of duck hepatitis B virus covalently closed circular DNA after in vitro infection of duck hepatocytes.
... virus envelope protein secretion has been reported for a human hepatoma cell line ...cccDNA amplification during the cell cycle progression of duck hepatocytes could correspond to low levels of pre-S ... See full document
7
Hepadnavirus P protein utilizes a tyrosine residue in the TP domain to prime reverse transcription.
... 2994 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Hepadnavirus DNA minus strands are covalently linked at their 5' terminus to the viral P gene product, which has bee[r] ... See full document
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Comparative Antigenicity and Immunogenicity of Hepadnavirus Core Proteins
... the various H-2 congenic strains. In the B10.M strain, for example, polyclonal anti-HBc antibodies were nonreactive with WHcAg but reacted with GScAg and AScAg with approx- imately 0.8% efficiency compared to HBcAg ... See full document
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Hepatitis B Virus Covalently Closed Circular DNA Formation in Immortalized Mouse Hepatocytes Associated with Nucleocapsid Destabilization
... Cell cultures. The AML12HBV10 cell line (20), derived from an immor- talized murine hepatocyte line (AML12) (21), and the HepAD38 cell line (a gift from Christoph Seeger at the Fox Chase Cancer Center) (22), de- rived ... See full document
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I. A study by electron microscopy of the RNA and RNA-DNA hybrids of HeLa mitochondria. II. Replication of closed circular mitochondrial DNA in mouse L cells
... ation of subcellular components from poliovirus- infccted and normal He La cell s. Interaction of HeLa cell proteins with RNA. The int e raction of closed circular DNA[r] ... See full document
154
Construction and Characterization of an in-vivo Linear Covalently Closed DNA Vector Production System
... extra-chromosomal, circular, covalently closed, double-stranded DNA mole- cules that often possess elements or sequences such as viral promoters or cloned coding sequences that could subject ... See full document
13
Detection, purification, and characterization of two species of covalently closed circular proviral DNA molecules of bovine leukemia virus.
... 3 0022-538X/83/031172-05$02.00/0 Copyright C 1983, American Society for Microbiology Detection, Purification, and Characterization of Two Species of Covalently Closed Circular Proviral D[r] ... See full document
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Elevated expression of serum soluble ST2 in clinical relapse after stopping long term Nucleos(t)ide analogue therapy for chronic hepatitis B
... Ninety-one CHB patients who enrolled in a prospective, single-center, observational study and underwent cessa- tion of NA therapy (Chinese Clinical Trial Registry num- ber: ChiCTR-OOC-17013970) were included in this ... See full document
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Virus-specific DNA in the cytoplasm of avian sarcoma virus-infected cells is a precursor to covalently closed circular viral DNA in the nucleus.
... VARMUS Department ofMicrobiology, University of California, San Francisco, California 94143 Three principal forms of viral DNA have been identified in cells infected with avian sarcoma v[r] ... See full document
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Covalently closed circular viral DNA formed from two types of linear DNA in woodchuck hepatitis virus-infected liver.
... Reconstruction amplification reactions were run on purified cloned WHV ...all amplification reactions were designed to detect artifacts that might have arisen from amplification of strands of relaxed ... See full document
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