[PDF] Top 20 Hepatitis C virus envelope proteins bind lactoferrin.
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Hepatitis C virus envelope proteins bind lactoferrin.
... Hepatitis C virus (HCV) has two envelope proteins, E1 and E2, which form a ...bovine lactoferrin, a multifunctional immu- nomodulator, binds two HCV envelope ...could ... See full document
6
Isolation, Cloning and Co-expression of Hepatitis C Virus Envelope Proteins: As Potential HCV Detecting Antigens
... of hepatitis C infection [25,26]. These proteins have been expressed in various expression ...these proteins in a bacterial host ...of envelope glycoproteins because of lacking in ... See full document
8
Hepatitis C Virus Envelope Glycoprotein E1 Forms Trimers at the Surface of the Virion
... velope proteins in the context of the viral particle, we analyzed them by SDS-PAGE under reducing conditions but without ther- mal ...influenza virus spike protein and the trimeric form of the Semliki ... See full document
14
Structure-Function Analysis of Hepatitis C Virus Envelope-CD81 Binding
... the virus interacts with its receptor represents an important factor in determining both the onset and the progression of viral infection, the first objective of this work was to determine the affinity of the ... See full document
7
Role of N-Linked Glycans in the Functions of Hepatitis C Virus Envelope Proteins Incorporated into Infectious Virions
... Hepatitis C virus (HCV) envelope glycoproteins are highly glycosylated, with generally 4 and 11 N-linked glycans on E1 and E2, ...HCV envelope glycoproteins incorpo- rated into ... See full document
11
Role of the Antigenic Loop of the Hepatitis B Virus Envelope Proteins in Infectivity of Hepatitis Delta Virus
... the envelope proteins, which are translated from three differ- ent in-frame start codons to a common stop codon ...the C-terminal S ...of envelope protein aggre- gates into the lumen of an ... See full document
7
Monoclonal Antibody AP33 Defines a Broadly Neutralizing Epitope on the Hepatitis C Virus E2 Envelope Glycoprotein
... Having established a panel of functional E1E2 clones rep- resentative of all the major genotypes, we went on to assess the capacity of the MAb AP33 and of rabbit antisera raised against the E2 protein of the H77 strain ... See full document
10
Antigenicity and Immunogenicity of Differentially Glycosylated Hepatitis C Virus E2 Envelope Proteins Expressed in Mammalian and Insect Cells
... We found that differences in N-glycosylation of sE2 produced in HEK293 versus Sf9 cells did not appreciably alter recognition by most HMAbs. Immunogenicity studies in mice showed that similar polyclonal antibody ... See full document
19
Functional Analysis of Hepatitis C Virus Envelope Proteins, Using a Cell-Cell Fusion Assay
... HCV envelope proteins are anchored to the membrane via a single C-terminal transmembrane (TM) domain and contain an N-terminal ectodomain (36) ...downstream proteins (E2 and p7, respectively), ... See full document
9
Identification of Conserved Residues in Hepatitis C Virus Envelope Glycoprotein E2 That Modulate Virus Dependence on CD81 and SRB1 Entry Factors
... on virus assembly also suggests that these res- idues are not involved in interactions with HCV glycoprotein E1, and the lack of effect on virus entry and interaction with CD81 is in agreement with their ... See full document
14
Production of Hepatitis C Virus Lacking the Envelope-Encoding Genes for Single-Cycle Infection by Providing Homologous Envelope Proteins or Vesicular Stomatitis Virus Glycoproteins in trans
... the envelope proteins of J6CF (genotype 2a) could rescue JFH1-based HCV ⌬ E production but that the envelope proteins of H77 (genotype 1a) and Con1 (genotype 1b) could not ...infectious ... See full document
10
Support of the Infectivity of Hepatitis Delta Virus Particles by the Envelope Proteins of Different Genotypes of Hepatitis B Virus
... The virions that contain on their outer side the PreS1 domain of the L protein, which bears the HBV receptor-interacting site, are potentially infectious (16). To access the fraction of the virions that bear the PreS1 ... See full document
13
Characterization of the Envelope Glycoproteins Associated with Infectious Hepatitis C Virus
... Hepatitis C is caused by an enveloped virus whose entry is mediated by two glycoproteins, namely, E1 and E2, which have been shown to assemble as a noncovalent ...pathogen, hepatitis C ... See full document
10
Hepatitis C Virus Core and Envelope Proteins Do Not Suppress the Host's Ability To Clear a Hepatic Viral Infection
... Immunohistochemistry and Western blotting. After being fixed in acetone and blocked with normal goat serum and avidin-biotin (Avidin/Biotin blocking kit; Vector Laboratories, Burlingame, Calif.), cryosections were ... See full document
7
Hepatitis C virus (HCV)-specific cytotoxic T lymphocytes recognize epitopes in the core and envelope proteins of HCV.
... Clone 92N-107, which recognized target cells infected with the E2/NS2 vector, was tested for its ability to lyse autologous target cells individually incubated with synthetic 20-aa HCV p[r] ... See full document
11
Role of N Glycosylation of Hepatitis B Virus Envelope Proteins in Morphogenesis and Infectivity of Hepatitis Delta Virus
... Effects of glycosylation-defective S, M, and L mutants on the infectivity of HDV particles. Because the M protein is dispens- able for the in vitro infectivity of HDV virions (31), we chose to ignore the role of ... See full document
5
Hepatitis C Virus and Vaccine Development
... viral envelope formation ...structural proteins stimulate the production of neutralizing antibodies and they might also serve as future vaccine candidates (17, ...the virus– host immune interactions ... See full document
9
Charged Residues in the Transmembrane Domains of Hepatitis C Virus Glycoproteins Play a Major Role in the Processing, Subcellular Localization, and Assembly of These Envelope Proteins
... the envelope proteins of this type of virus are translated from a single coding region implies that internal signal peptides must be ...the C terminus of the immature form of the capsid ... See full document
11
Replication-Competent Recombinant Vesicular Stomatitis Virus Encoding Hepatitis C Virus Envelope Proteins
... as by the inhibition of binding of purified E2 protein to hCD81 (24, 53) and of HCV-LP to target cells (59). Sera from patients chronically infected with HCV and experimentally infected chimpanzees were shown to ... See full document
12
Identification of Novel Functions for Hepatitis C Virus Envelope Glycoprotein E1 in Virus Entry and Assembly
... E1E2 proteins were then incubated with CD81-LEL complexed with glutathione beads overnight at ...the proteins of ...HCV envelope glycoproteins was then detected by Western ... See full document
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