[PDF] Top 20 Hepatitis C virus polyprotein processing: kinetics and mutagenic analysis of serine proteinase-dependent cleavage.
Has 10000 "Hepatitis C virus polyprotein processing: kinetics and mutagenic analysis of serine proteinase-dependent cleavage." found on our website. Below are the top 20 most common "Hepatitis C virus polyprotein processing: kinetics and mutagenic analysis of serine proteinase-dependent cleavage.".
Hepatitis C virus polyprotein processing: kinetics and mutagenic analysis of serine proteinase-dependent cleavage.
... Amino acids substitutions to asparagine at both the P1 and P1' positions of the putative 3/4A, 4A/4B, 4B/5A cleavage sites of the nonstructural precursor polyprotein were independently i[r] ... See full document
5
Substrate determinants for cleavage in cis and in trans by the hepatitis C virus NS3 proteinase.
... the hepatitis C virus polyprotein is accomplished by a series of cotranslational and posttranslational cleavages mediated by host cell signalases and two virally encoded ...NS3 ... See full document
8
A central region in the hepatitis C virus NS4A protein allows formation of an active NS3-NS4A serine proteinase complex in vivo and in vitro.
... NS3 serine proteinase to cleave at the 4B/5A site is still ...NS3 serine proteinase domain expressed in the absence of NS4A is active and can cleave at the 5A/5B site efficiently in vivo (3, ... See full document
8
Bovine viral diarrhea virus NS3 serine proteinase: polyprotein cleavage sites, cofactor requirements, and molecular model of an enzyme essential for pestivirus replication.
... NS3 serine proteinase domain were required for pro- cessing at the 5A/5B site ...for cleavage at both the 4B/5A and the 5A/5B ...NS3 serine proteinase domain (7, 25, 51, 81) and ... See full document
11
NS5A Domain 1 and Polyprotein Cleavage Kinetics Are Critical for Induction of Double Membrane Vesicles Associated with Hepatitis C Virus Replication
... Regulated cleavage at the NS4B-5A junction determines DMV ...that processing intermediates serve distinct functions in the replica- tion ...HCV, polyprotein processing by NS3-4A is a tightly ... See full document
12
Calcium-Dependent Calpain Proteases Are Implicated in Processing of the Hepatitis C Virus NS5A Protein
... HSV-1 virus strain LaL ...blot analysis by using either a rabbit polyclonal antibody raised against an MBP-NS5A recombinant protein, as described in Materials and Methods, or a commer- cially available NS5A ... See full document
14
Characterization of the hepatitis C virus-encoded serine proteinase: determination of proteinase-dependent polyprotein cleavage sites.
... To further refine the map of the HCV nonstructural proteins and to determine the sequence at the four potential NS3 proteinase cleavage sites, the N-terminal sequences of NS4A, NS4B, NS5[r] ... See full document
12
Complex formation between the NS3 serine-type proteinase of the hepatitis C virus and NS4A and its importance for polyprotein maturation.
... 18). Cleavage at the NS4B/5A junction apparently was not affected ...reduced processing efficiency, were found with the proteinase lacking 15 amino acids ...22). Proteinase activation by NS4A ... See full document
10
Hepatitis C virus NS3 serine proteinase: trans-cleavage requirements and processing kinetics.
... 23, the pestivirus plO protein may be the functional homolog of HCV NS4A, because sequences in this region of the pestivirus polyprotein appear to be required for the serine proteinase-d[r] ... See full document
11
The NS3 proteinase domain of hepatitis C virus is a zinc-containing enzyme.
... NS3 proteinase (residues 1 to 181) contains three histidine residues, His-57, His-110, and ...mutational analysis to be essential for NS3 proteinase ac- tivity (9, 16), consistent with its being a ... See full document
6
Improving Proteolytic Cleavage at the 3A/3B Site of the Hepatitis A Virus Polyprotein Impairs Processing and Particle Formation, and the Impairment Can Be Complemented intrans by 3AB and 3ABC
... proteolytic cleavage sites within the P3 ...two cleavage sites, the cDNA containing a single nucleotide change was mutated at the second site by the same ... See full document
12
Active foamy virus proteinase is essential for virus infectivity but not for formation of a Pol polyprotein.
... and C of ...foamy virus type 1 particles as a single 81-kDa protein ...leukemia virus, in which both RT and RNase H activities reside in a single 80-kDa protein (29, ...the C-terminal domain ... See full document
5
Analysis of Serine Codon Conservation Reveals Diverse Phenotypic Constraints on Hepatitis C Virus Glycoprotein Evolution
... Deletion of partial E1E2 sequences (16, 28), intergenotypic do- main swapping (15, 44), and mutations which abrogate glycopro- tein heterodimerization (17, 45) can all result in assembly-im- paired phenotypes. ... See full document
12
Proteinase 3C-mediated processing of VP1-2A of two hepatitis A virus strains: in vivo evidence for cleavage at amino acid position 273/274 of VP1.
... vaccinia virus T7 system described before ...VP1-2A cleavage site are ...273 cleavage site, whereas pEXT7-HM/HM-P1-2A(E/S) contains an E/S pair at this ... See full document
5
Mutagenesis of the yellow fever virus NS2B/3 cleavage site: determinants of cleavage site specificity and effects on polyprotein processing and viral replication.
... on cleavage efficiency by mutations introduced at the P3 and P4 positions of the 2B/3 cleavage site were ...reduced cleavage efficiency, whereas others ap- peared to enhance cleavage, ... See full document
6
Processing of pestivirus polyprotein: cleavage site between autoprotease and nucleocapsid protein of classical swine fever virus.
... A Alignment of the amino acid sequences of CSFV strains Alfort 12 and Brescia 14; BVDV strains NADL 5, Osloss 15, SD-1 7, and Pe5I5CP 13; and border disease of sheep virus BDV strains 13[r] ... See full document
8
The hepatitis A virus polyprotein expressed by a recombinant vaccinia virus undergoes proteolytic processing and assembly into viruslike particles.
... Fifty microliters of rV-ORF and wild-type vaccinia virus-infected cell lysates was incubated with 7.5 RI1 of human polyclonal anti-HAV serum JC-pcAb or nonimmune serum BV-niAb in a total[r] ... See full document
8
Mutational Analysis of the Rubella Virus Nonstructural Polyprotein and Its Cleavage Products in Virus Replication and RNA Synthesis
... and virus yields. The more NSP cleavage is impaired, the less pos- itive-strand RNA is ...NSP cleavage may not be the cause for the differential production of subgenomic and genomic RNAs in ... See full document
9
A New Role for ns Polyprotein Cleavage in Sindbis Virus Replication
... sequential processing of the nonstructural polyproteins P1234 and ...following processing steps make nsP1 ⴙ P23 ⴙ nsP4, and later nsP1 ⴙ nsP2 ⴙ nsP3 ⴙ ...ns polyprotein processing. In this ... See full document
14
Mapping of the Feline Calicivirus Proteinase Responsible for Autocatalytic Processing of the Nonstructural Polyprotein and Identification of a Stable Proteinase-Polymerase Precursor Protein
... a C-terminal His tag, and antiserum was raised in a guinea ...of virus infection or translation products of the ORF1 clone showed that an uncleaved 78-kDa Pro-Pol protein accumulated in both lysates ...the ... See full document
8
Related subjects