[PDF] Top 20 The Hepatitis C Virus NS4B Protein Can trans-Complement Viral RNA Replication and Modulates Production of Infectious Virus

... produces infectious HCV from tissue culture cells now permits studies of the require- ments for virus assembly and ...of viral structural components, it has been dem- onstrated recently that both NS3 ... See full document

... core protein perfectly colocalized to the surface of LD, and this cell compartment likely is a crucial platform for HCV assembly (2, 37, 39, 46, 50, ...core protein and NS3 at the surface of LD ...and ... See full document

... the replication of hepatitis C virus (HCV) have been facilitated by the development of selectable subgenomic replicons replicating in the human hepatoma cell line Huh-7 at a surprisingly high ... See full document

... HCV RNA replica- ...serine/threonine protein kinases that block NS5A hyperphosphorylation facili- tate replication of a non-culture-adapted replicon (3, ...HCV RNA replication by ... See full document

... both virus and host cells may adapt during persistent HCV infection in cell cultures, such that cells become resistant to infection due to reduced expression of the viral coreceptor CD81 ...HCV ... See full document

... of viral and host cell factors involved in HCV rep- lication (for a recent review, see reference ...plus-strand RNA viruses, HCV induces intracellular membrane alterations and repli- cates its genome in ... See full document

... 50-fold-less infectious virus (data not ...E1 protein. This substi- tution enhanced infectious particle production (data not shown) and was included in all genomes ...in ... See full document

... NS5A protein, and the main viral assembly protein, HCV ...JFH-FLAG virus showed the expected punctate staining of ...and C, panels a to c), a lipid dye that specifically stains ... See full document

... epatitis C virus (HCV) is one of the major agents responsible for causing severe liver diseases, including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma (1, ...positive-stranded ... See full document

... the viral polyprotein ...increased infectious virus production in the context of a wild-type ...of viral fitness, thereby suppressing the defective core ...normal RNA accumula- ... See full document

... simian virus 40 late promoter. On transfection into HuH-7 cells, the plasmid can direct replication of the viral genome and expression of the two species of ...the production of both ... See full document

... of viral RNA replication, as suggested by similar levels of positive-stranded HCV RNA in the cells transfected with wild-type and mutant RNAs, which were quantified by both RPA and qRT-PCR ... See full document

... that protein expression from monocistronic, full- length viral RNAs containing a mutation in the S1 site that ablated the miR-122 effect on RNA abundance was not mea- surably reduced from that ... See full document

... the production of infectious J6-JFH1 virus was analyzed with a few ...JFH1 NS4B and a previous re- port showed that R192 is dispensable for JFH1 replication and virus ... See full document

... Con1 NS4B enhance H77 RNA synthesis in Huh- ...Con1 NS4B protein func- tions more efficiently in the context of the H77-encoded rep- lication machinery than the native H77 ...NS4B. ... See full document

... ABSTRACT Hepatitis C virus (HCV) strain JFH-1, which belongs to genotype 2a, repli- cates autonomously in cultured cells, whereas another genotype 2a strain, J6CF, does ...establish ... See full document

... of NS4B foci. One important role of NS4B in HCV replication is its ability to induce a distinct ultrastructural membrane alteration called the membranous web (16, 37), which appears as dots or foci ... See full document

... of NS4B has not been resolved, although specific amino acid substitutions at I131 and other residues within this region abol- ish Con1 RNA replication ...of NS4B between Con1 and H77 revealed ... See full document

... wild-type RNA. For AV-FL-Luc-5 ⬘ rzm-2Afs RNA, polyprotein processing was slower than in the other RNAs ...80-kDa protein was accumu- lated ...AV-FL-2Afs RNA, an abnormal processing pattern ... See full document

... in NS4B: are both cysteine residues fully modified or only partially modified by palmitic acid? To answer this question, we tried other biochemical methods to define the extent of modification of the cysteine ... See full document