[PDF] Top 20 Hepatitis C Virus Subgenomic Replicon Requires an Active NS3 RNA Helicase
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Hepatitis C Virus Subgenomic Replicon Requires an Active NS3 RNA Helicase
... purified NS3 helicases containing an Ala substitution for either R393 or F438 do not unwind RNA, even though they retain some ability to unwind DNA and retain the same level of nucleic acid stimulated- ... See full document
8
Structurally Conserved Amino Acid W501 Is Required for RNA Helicase Activity but Is Not Essential for DNA Helicase Activity of Hepatitis C Virus NS3 Protein
... al. requires two capping residues, W501 and V432, which intercalate the base of the substrate at the 3⬘ and 5⬘ end, ...also requires two major ATP binding sites, one at Walker motifs A and B in domain 1 and ... See full document
12
Cytoskeletal Requirements for Hepatitis C Virus (HCV) RNA Synthesis in the HCV Replicon Cell Culture System
... Hepatitis C virus (HCV) infection is the leading cause of liver transplantation in the United States, with sequelae includ- ing liver fibrosis, cirrhosis, and hepatocellular carcinoma (re- viewed in ... See full document
8
NS3 Helicase Domains Involved in Infectious Intracellular Hepatitis C Virus Particle Assembly
... of virus in RNA-transfected cells. The abundance of HCV RNA and viral proteins could be affected not only by RNA replication but also by spread of virus within a ...porting RNA ... See full document
16
Identification of the Hepatitis C Virus RNA Replication Complex in Huh-7 Cells Harboring Subgenomic Replicons
... dates active RNA ...plus-strand RNA viruses where single pro- teins expressed alone or in the context of the entire open reading frame induced membrane alterations very similar to membrane changes ... See full document
6
Highly Permissive Cell Lines for Subgenomic and Genomic Hepatitis C Virus RNA Replication
... selectable replicon pHCVrep1bBartMan/AvaII (SG-Neo [wild type, [wt]) ...and subgenomic constructs, NS5B polymerase-defective derivatives carrying a triple amino acid substitution were generated, changing ... See full document
14
NS3 from Hepatitis C Virus Strain JFH-1 Is an Unusually Robust Helicase That Is Primed To Bind and Unwind Viral RNA
... (SF2) helicase, including the conserved sequence motifs for ATP hydrolysis and RNA recognition ...paradigmatic helicase domains, each of which adopts the RecA fold architecture that is typical for ... See full document
16
Replication of a hepatitis C virus replicon clone in mouse cells
... of RNA into ...neo-expressing replicon RNA led to the dis- covery that efficient replication of most HCV replicons in cell culture requires adaptive mutations in the viral genome ...1b ... See full document
9
Effect of Alpha Interferon on the Hepatitis C Virus Replicon
... Hepatitis C virus (HCV) causes persistent infection in ap- proximately 80% of infected adults and variable and severe liver disease in an estimated 70% of those who cannot clear the virus ... See full document
8
Protease Inhibitors Block Multiple Functions of the NS3/4A Protease-Helicase during the Hepatitis C Virus Life Cycle
... Hepatitis C virus (HCV) NS3 is a multifunctional protein composed of a protease domain and a helicase domain linked by a flex- ible ...in RNA replication. Helicase activ- ... See full document
9
Stimulation of Hepatitis C Virus (HCV) Nonstructural Protein 3 (NS3) Helicase Activity by the NS3 Protease Domain and by HCV RNA-Dependent RNA Polymerase
... HCV NS3 is able to unwind partially duplex nucleic acids (RNA-RNA, RNA-DNA, or DNA-DNA) without exhibiting substrate specificity in vitro (19, 20, ...Also, NS3 helicase ... See full document
11
Unmasking the Active Helicase Conformation of Nonstructural Protein 3 from Hepatitis C Virus
... the hepatitis C virus (HCV) replication complex (2, ...the C-terminal two- thirds of the protein ...the NS3 protease activity cleaves and releases downstream viral proteins from the ... See full document
11
DDX3 DEAD-Box RNA Helicase Is Required for Hepatitis C Virus RNA Replication
... double-stranded RNA and separate the RNA strands or might contribute to the function of HCV NS3 ...of subgenomic replicon RNA was also partially suppressed in DDX3 knockdown ... See full document
5
Nonhepatic Cell Lines HeLa and 293 Support Efficient Replication of the Hepatitis C Virus Genotype 2a Subgenomic Replicon
... lomyocarditis virus (EMCV) IRES directs the expression of HCV nonstructural (NS) proteins NS3 to ...HCV replicon in non-hepatocyte-derived cell lines have been made previously (1, ...efficient ... See full document
5
A Genetic Interaction between Hepatitis C Virus NS4B and NS3 Is Important for RNA Replication
... C1/H C 34 chi- mera to levels greater than that of C1/WT ...additional NS3 mutations were also tested (P1115R, E1202G, D1431N, S1560G, and P1115R plus S1560G) (Table 2) and shown to rescue C1/H N 47 , C1/H ... See full document
13
The NS4A Protein of Hepatitis C Virus Promotes RNA-Coupled ATP Hydrolysis by the NS3 Helicase
... that requires at least two ...RhlB requires the RNase E cofactor to stimulate its ATPase activity and localize it to an unwinding substrate (15, ...Dbp8 requires the Esf2 cofactor for ATPase ... See full document
8
Structure-Based Mutagenesis Study of Hepatitis C Virus NS3 Helicase
... of RNA binding, (iv) inhibition of unwinding by sterically blocking translocation of helicase along the polynucleotide chain, and (v) inhibition of the coupling of ATP hydrolysis to ...HCV NS3 ... See full document
10
Hepatitis C Virus RNA Synthesis in a Cell-Free System Isolated from Replicon-Containing Hepatoma Cells
... agents, virus-specific processes are often ...sess RNA synthetic activity (4, 12, 26, 29, 33, ...genome RNA as a template ...that NS3 is part of the replicase and that the associated ... See full document
9
Structure-Based Mutational Analysis of the Hepatitis C Virus NS3 Helicase
... affect RNA helicase activity and the implications. Unwinding of RNA is an ATP-dependent process which requires ATP binding, ATP hydrolysis, and RNA ...impede RNA helicase ... See full document
9
The Hepatitis C Virus NS3 Protein: A Model RNA Helicase and Potential Drug Target
... HCV helicase inhibitors were nucleoside ...the helicase in addition to the conserved Walker ...the helicase functions as a dimer with ATP bound tightly to the interface between domains 1 and 2 and ... See full document
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