[PDF] Top 20 Herpes Simplex Virus 1 pUL34 Plays a Critical Role in Cell-to-Cell Spread of Virus in Addition to Its Role in Virus Replication

... no pUL34, where the nuclear envelope is generally of uniform shape (27, ...UL34-null virus, we observed no cell surface virions or cytoplasmic egress intermediates despite the pres- ence of numerous ... See full document

... that plays a central role in cellular responses to a broad range of stress factors through its regulation of a variety of cellular pathways, such as apoptosis, cell cycle, cellular senescence, ... See full document

... the virus to evade or manipulate the host immune ...though its RING finger domain (15, ...In addition, a recombinant HSV-1 strain lacking the first 147 amino acids of ...viral replication ... See full document

... p32 cell protein is primarily localized in the mitochon- drial matrix, but it has also been reported to be present at the cell surface and in the nucleus and cytoplasm (24, ...In addition, p32 has ... See full document

... to spread from cell to cell in the presence of immune effectors is a conserved property of ...that cell-to-cell spread (CCS) between epithelial cells in vitro has two mechanistic ... See full document

... for virus entry and which are much more limited in number than the sites to which the virus can ...UV-inactivated virus particles per cell were resistant to infec- tion with ...of virus ... See full document

... direct cell-to-cell spread of virions by fusion of neighboring cells (reviewed in reference ...11 herpes simplex virus type 1 (HSV-1)-encoded glycoproteins have ... See full document

... gD-expressing cell lines and the S140N mutants were the least efficient among the mutants tested in overcom- ing the gD-mediated restriction of BJ cells ...regions 1, 3 and 4, is essential for the process ... See full document

... minor role in viral replication in cell cultures, we demonstrated here that the nuclease activities of UL12 played a major role in neurovirulence in mice following intracerebral ...on ... See full document

... of critical amino acids within the so-called Walker motifs were shown to abolish helicase activity, both in vitro and in vivo ...In addition, HSV mutants carrying single amino acid substitutions in either ... See full document

... redistributes pUL34 into cytoplasmic puncta that colocalize with a capsid ...that pUL34, which is a structural component of the primary but not the mature virion (14, 27, 31, 42, 45), should also be ... See full document

... the role of EVs in myelination and neuron-glia communication (33, 35, ...enhance spread, or evade the host immune response (48, ...for virus replication and immune evasion ...In ... See full document

... Herpes simplex virus (HSV) exhibits altered gene regulation in neuronal compared to nonneuronal ...of replication (oriS and oriL) is a critical step in the upregulation of ... See full document

... ICP34.5-deficient virus in culture are due to high levels of phosphorylated eIF2 ␣ and subsequent shutoff of viral protein ...the critical ICP34.5-dependent function for efficient HSV-1 repli- ... See full document

... they were propagated on, and their titers were determined on, 293 cells. Antibodies. Monoclonal antibody (MAb) 3104, specific for gI, and MAb 3114, specific for gE (25), were gifts of Anne Cross and Nigel Stow (Institute ... See full document

... for its gD-binding activity. To examine whether the interaction of nectin-1␣ with afadin affects the gD-binding activity of nec- tin-1␣, we compared the activities among nectin-1␣-L, nectin- ... See full document

... In addition, survival rates determined from plating efficiencies of the various cell mutants after incubations at 39.5°C for the time periods corresponding 42 Downloaded from http://jvi.[r] ... See full document

... to cell junc- tions? Cellular membrane proteins are sorted to basolateral surfaces and separated from apical proteins in the TGN by recognition of sorting motifs (tyrosine, dileucine, and acidic domains) that ... See full document

... infection. Cell-to-cell fusion between infected cells and the Jurkat-LTR- luc cells was quantified by measuring the luciferase activity of the lysed ...initiated cell-to-cell fusion with a ... See full document

... The tegument of a mature virion consists of at least 20 proteins in various amounts, irregularly distributed (38). In contrast, the PEV has a spherical shell of uniform thickness, composed of the NECs clustered in a ... See full document