[PDF] Top 20 High Throughput Multiplex Serological Testing in Microbiology
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High Throughput Multiplex Serological Testing in Microbiology
... a multiplex serological technique called Suspension Multiplex Immunoassay (SMIA) (Figure 2) ...of high throughput multiplex testing does not need to be ... See full document
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Genotyping of Toxoplasma gondii by Multiplex PCR and Peptide Based Serological Testing of Samples from Infants in Poland Diagnosed with Congenital Toxoplasmosis
... PCR, serological analysis indicated a majority of these were also type ...that high parasite concentration is primarily responsible for the severity of congenital ...and serological testing ... See full document
8
Automated Multiplex Assay System for Simultaneous Detection of Hepatitis B Virus DNA, Hepatitis C Virus RNA, and Human Immunodeficiency Virus Type 1 RNA
... A high-throughput and high-sensitivity automated multiplex assay is needed to meet the testing requirements of large blood testing ...automated, high-throughput, ... See full document
9
High Throughput Molecular Determination of Salmonella enterica Serovars by Use of Multiplex PCR and Capillary Electrophoresis Analysis
... single multiplex PCR targeting 16 genomic ...of multiplex reactions and single PCRs that could discriminate the common serotypes of Salmonella en- terica ...12 multiplex PCRs, each with eight ... See full document
10
Development and Standardization of a High-Throughput Multiplex Immunoassay for the Simultaneous Quantification of Specific Antibodies to Five Respiratory Syncytial Virus Proteins
... An important advantage of the MIA over MN and ELISA is the 4-log linear range, allowing the reliable quantitation of samples over a wider concentration range and reducing the need of (re)testing samples at a ... See full document
12
Outbreak Investigation Using High Throughput Genome Sequencing within a Diagnostic Microbiology Laboratory
... Resistance determinants. No automated method to screen NGS data for a wide range of antimicrobial resistance determinants is freely avail- able. Therefore, we manually explored the partially assembled genomes for ... See full document
6
Clinical Evaluation of the New High Throughput Luminex NxTAG Respiratory Pathogen Panel Assay for Multiplex Respiratory Pathogen Detection
... NxTAG-RPP multiplex PCR assay through the testing of 284 clinical respiratory ...the high number of false-positive results (11 false-positive ... See full document
6
Serological Array-in-Well Multiplex Assay Reveals a High Rate of Respiratory Virus Infections and Reinfections in Young Children
... ABSTRACT Serological assays are used to diagnose and characterize host immune responses against microbial ...facilitate high- throughput immunoassays of antibody detection against multiple pathogens ... See full document
14
Clinical Utility of Advanced Microbiology Testing Tools
... (e.g., high risk for respiratory conditions) with two diagnostic arms ...to multiplex panels) may be able to reach significance with 100 ...(e.g., high risk, low risk, and the general population) and ... See full document
13
Serological Analysis of Tuberculosis in Goats by Use of the Enferplex Caprine TB Multiplex Test
... serology testing showed that IgG antibodies were present in both serum and milk from 100% of skin test-positive animals and in serum and milk from ...direct serological cross- reactions were found. ... See full document
13
Multiplex lateral flow immunoassay: an overview of strategies towards high-throughput point-of-need testing
... A tentative to overcome limitations due to sample availability and cost of production has been proposed by Schenk et al., who described the first steps aimed at developing a ‘multi-channel strip’ for the simultaneous ... See full document
12
High throughput malaria serosurveillance using a one step multiplex bead assay
... DBS). Serological estimates from a serosurvey in Haiti of IgG prevalence in the study population were similar regardless of protocol or sample type, indicating that the novel OneStep proto- col could generate ... See full document
10
Rapid, High Throughput, Multiplex, Real Time PCR for Identification of Mutations in the cyp51A Gene of Aspergillus fumigatus That Confer Resistance to Itraconazole
... Multiplex real-time PCR detection of Gly54 mutations. Once target-specific hybridization with complement DNA tar- get was validated for each beacon, we investigated the possi- bility of combining them in a ... See full document
9
Performance of the Enferplex TB Assay with Cattle in Great Britain and Assessment of Its Suitability as a Test To Distinguish Infected and Vaccinated Animals
... novel multiplex serological test with sera collected from 96 skin-tested animals with bovine tuberculosis, 93 TB-free animals, and 39 cattle vaccinated with Mycobacterium bovis ...this serological ... See full document
5
Prospective Evaluation of ResistancePlus MG, a New Multiplex Quantitative PCR Assay for Detection of Mycoplasma genitalium and Macrolide Resistance
... resistance testing to inform management of ...resistance testing and individualize care have the potential to greatly improve microbial cure, to promote antimicrobial stewardship, and to reduce clinic ... See full document
5
Single-Antigen Serological Testing for Bovine Tuberculosis
... Multiantigen testing has the potential to increase diagnostic sensitivity, but it can also create new challenges. Test costs increase proportionately with increasing numbers of antigen reagents, as does the risk ... See full document
5
Drug Susceptibility Testing of Mycobacterium tuberculosis Complex by Use of a High Throughput, Reproducible, Absolute Concentration Method
... The quality of the 25-well DST method proves to be of a high standard. If the plates are kept for no longer than 3 weeks, the five control strains yield highly standardized MICs. In the second-line quality ... See full document
7
Proficiency Testing of Virus Diagnostics Based on Bioinformatics Analysis of Simulated In Silico High Throughput Sequencing Data Sets
... scalable high- performance national virtual machine, whereas the slowest workflow (participant 4; 216 h) involved calculation on a personal computer through an external public server where bioinformatics software ... See full document
12
Mapping Neurotransmitter Identity in the Whole-Mount Drosophila Brain Using Multiplex High-Throughput Fluorescence in Situ Hybridization
... for high-throughput detection of eight common neurotransmitter phenotypes in Drosophila ...FISH throughput by processing samples mounted on coverslips and optimizing fluorophore choice for each probe ... See full document
10
Development of novel multiplex microsatellite polymerase chain reactions to enable high throughput population genetic studies of Schistosoma haematobium
... and multiplex amplicons were visualised on 3 % gel red agarose gels before 2 μl of 1: 50 dilutions were mixed with ...The multiplex PCRs proved robust giving identical peak scores in repeated reactions, in ... See full document
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