[PDF] Top 20 Human immunodeficiency virus type 1 preintegration complexes: studies of organization and composition.
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Human immunodeficiency virus type 1 preintegration complexes: studies of organization and composition.
... Active preintegration complexes contain processed 3 * ...Earlier studies presented evidence of 39-end processing in viral cDNA isolated from active Moloney MLV (MoMLV) PICs (6, 23) or from cells ... See full document
9
Intracytoplasmic maturation of the human immunodeficiency virus type 1 reverse transcription complexes determines their capacity to integrate into chromatin
... in virus- specific complexes early after infection, but it was absent in cRTCs analyzed at later time points and in nuclear RTCs (nRTCs) [4,7] The composition of the HIV-1 nPICs is still ... See full document
12
Nuclear Localization of Human Immunodeficiency Virus Type 1 Preintegration Complexes (PICs): V165A and R166A Are Pleiotropic Integrase Mutants Primarily Defective for Integration, Not PIC Nuclear Import
... for 1 h at ambient temperature with anti-FLAG M2 anti- body (Sigma-Aldrich, ...diluted 1:500 in 5% ...for 1 h with fluorescein isothiocyanate- conjugated donkey anti-mouse antibody (Jackson ... See full document
10
Evidence for a Functional Link between Uncoating of the Human Immunodeficiency Virus Type 1 Core and Nuclear Import of the Viral Preintegration Complex
... Other studies have failed to detect CA in association with cytoplasmic reverse transcription complexes (RTCs) recovered from cells soon after inoculation (21, ...transcription complexes contained CA, ... See full document
9
Characterization of Intracellular Reverse Transcription Complexes of Human Immunodeficiency Virus Type 1
... scopic studies also indicated that the HIV core is disrupted in the process of virus internalization, while MoMLV cores per- sist longer inside the infected cells (15, ...since complexes isolated ... See full document
10
Nuclear Import of the Preintegration Complex Is Blocked upon Infection by Human Immunodeficiency Virus Type 1 in Mouse Cells
... Recent studies have shown that nonclassical NLSs are necessary and sufficient to locate the viral PIC into the nuclei ...of human cells ...in human cells and defective in mouse ...Friend virus ... See full document
12
Recombinant Human Immunodeficiency Virus Type 1 Integrase Exhibits a Capacity for Full-Site Integration In Vitro That Is Comparable to That of Purified Preintegration Complexes from Virus-Infected Cells
... synaptic complexes thus holding the two viral DNA ends cor- rectly together may be enhanced by the addition of host factors like BAF found in both MLV (33, 48) and HIV-1 PIC (12, ... See full document
9
Structural studies of human immunodeficiency virus type 1 by cryo electron tomography
... From this work we conclude that (1) the new flip-flop holder succeeds in making the collection of dual-axis tilt-series of cryosamples convenient and routine, (2) including the second tilt-series does result in ... See full document
139
Independent Segregation of Human Immunodeficiency Virus Type 1 Gag Protein Complexes and Lipid Rafts
... influenza virus literature (32) and chose several well-charac- terized raft markers as ...mapping studies were unambiguous, we felt that the gradient conditions may have introduced some bias into the ... See full document
11
Hsp40 Facilitates Nuclear Import of the Human Immunodeficiency Virus Type 2 Vpx-Mediated Preintegration Complex
... Vpx colocalizes with Hsp40/DnaJB6 by indirect immuno- fluorescence. Interestingly, the nuclear localization of Vpx was modulated by Hsp40/DnaJB6. Overexpression of Hsp40/ DnaJB6 increased Vpx nuclear accumulation, ... See full document
9
Structure-function studies of the human immunodeficiency virus type 1 matrix protein, p17.
... p17 is also involved in the early stages of a new infection. Certain C-terminal mutants of p17 have been shown to be defective in the ability to synthesize viral DNA following in- fection, suggesting a role for p17 in ... See full document
10
The human immunodeficiency virus type 1 Vif protein modulates the postpenetration stability of viral nucleoprotein complexes.
... of human immunodeficiency virus type 1 is absolutely required for productive infection of primary cells derived from human blood and certain immortalized T lymphoid cells, for ... See full document
9
Pseudodiploid Genome Organization Aids Full-Length Human Immunodeficiency Virus Type 1 DNA Synthesis
... Based on the observations reported here and the remarkable frequency of template switching observed during HIV DNA synthesis (roughly one crossover per kilobase [25, 32, 44]), we propose a model wherein the second ... See full document
9
Simian Immunodeficiency Virus Integration Preference Is Similar to That of Human Immunodeficiency Virus Type 1
... of preintegration complexes (1, 4, 8, 22), including integrase, nucleocapsid protein (4, 28), and the at- tachment sites of the reverse-transcribed genome, contribute to the ability of ... See full document
6
Formation of Virus Assembly Intermediate Complexes in the Cytoplasm by Wild-Type and Assembly-Defective Mutant Human Immunodeficiency Virus Type 1 and Their Association with Membranes
... precursor complexes in T cells expressing wild-type or myristylation-neg- ative HIV-1 Gag and Gag-Pol precursors ...with virus infectivity (42). Furthermore, other studies have ... See full document
9
Leucine-Specific, Functional Interactions between Human Immunodeficiency Virus Type 1 Nef and Adaptor Protein Complexes
... AP complexes from cytoplasmic lysates was tested with a GST pull-down ... 1 in the yeast two-hybrid assay yet was devoid of biologic ... 1 as provided by a tyrosine-based motif is insufficient for ... See full document
13
Vif and the p55Gag Polyprotein of Human Immunodeficiency Virus Type 1 Are Present in Colocalizing Membrane-Free Cytoplasmic Complexes
... nucleoprotein complexes (also known as preintegration com- plexes) prior to DNA integration (28, ...in virus-producing cells for its effect on infectivity to be exerted (23, 56), this finding has led ... See full document
8
Human immunodeficiency virus type 2 preintegration complexes: activities in vitro and response to inhibitors.
... To examine the structure of junctions between HIV-2 cDNA and target DNA created by integration, nine pairs of junctions were cloned and sequenced. Integration products were gener- ated by incubating HIV-2 PICs with a ... See full document
6
Molecular Characterization of Preintegration Latency in Human Immunodeficiency Virus Type 1 Infection
... and virus gene expression, events that are not generally observed in resting T cells in the absence of an activating stimulus ...produce virus in the absence of antigenic stimulation, perhaps due to the ... See full document
14
Reduced nuclear import of human immunodeficiency virus type 1 preintegration complexes in the presence of a prototypic nuclear targeting signal.
... This result was consistent with the observation that circularization of viral DNA could be prevented in the presence of P-LYS NLS peptides and supports the view that a prototypic NLS can[r] ... See full document
5
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