[PDF] Top 20 Identification and Characterization of a Herpes Simplex Virus Gene Product Required for Encapsidation of Virus DNA
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Identification and Characterization of a Herpes Simplex Virus Gene Product Required for Encapsidation of Virus DNA
... In this study, we report the characterization of an HSV-1 mutant that has a temperature-sensitive processing defect in the nucleocapsid structural polypeptide p40 and fails to encapsidat[r] ... See full document
9
Herpes Simplex Virus Type 1 UL34 Gene Product Is Required for Viral Envelopment
... U of RQ1 DNase (Promega) and allowing the reaction to proceed at 37°C for 30 min. The nuclear extract was fractionated by centrifugation on a 10-ml 15 to 45% sucrose gradient made up in PBS at 35,000 rpm for 30 min. ... See full document
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DNA Mismatch Repair Proteins Are Required for Efficient Herpes Simplex Virus 1 Replication
... in virus yield (10- and 20-fold, respec- tively); however, analysis of the block in the virus life cycle revealed that MLH1 acts at a step prior to that of ...IE gene expression and a failure to make ... See full document
13
The Product of the Herpes Simplex Virus Type 1 UL25 Gene Is Required for Encapsidation but Not for Cleavage of Replicated Viral DNA
... UL25 gene. The 580-amino-acid open reading frame for the UL25 gene extends from nucleo- tides 48813 (ATG) to 50553 (TAG) in the HSV-1 genome ...UL25 gene was cloned into a vector and the single NotI ... See full document
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Herpes simplex virus type 1 DNA synthesis requires the product of the UL8 gene: isolation and characterization of an ICP6::lacZ insertion mutation.
... Herpes simplex virus type 1-induced ribonucleotide reductase activity is dispensable for virus growth and DNA synthesis: isolation and characterization of an ICP6 lacZ insertion mutant..[r] ... See full document
9
Role of the UL25 Gene Product in Packaging DNA into the Herpes Simplex Virus Capsid: Location of UL25 Product in the Capsid and Demonstration that It Binds DNA
... packaging DNA is similar to that of double-stranded DNA (dsDNA) bacteriophages such as T4, P22, and lambda (9, 27, 28, ...the gene products gp4, gp10, and gp26 of phage ...mature DNA within ... See full document
10
The product of the UL12.5 gene of herpes simplex virus type 1 is a capsid-associated nuclease.
... of herpes simplex virus type 1 (HSV-1) encodes a deoxyribonuclease that is frequently referred to as alkaline nuclease (AN) because of its high pH ...latter gene to initiate translation of a ... See full document
9
Novel Class of Thiourea Compounds That Inhibit Herpes Simplex Virus Type 1 DNA Cleavage and Encapsidation: Resistance Maps to the UL6 Gene
... UL6 gene products from HSV-1 and HSV-2 is ...an encapsidation complex. These interactions can influence the encapsidation process at various levels: (i) the correct subcellular localization of the ... See full document
8
The UL6 Gene Product Forms the Portal for Entry of DNA into the Herpes Simplex Virus Capsid
... HSV-1 DNA encapsidation resembles that observed in double-stranded DNA (dsDNA) bacterio- phages, such as P22, T7, and ...the DNA enters and exits the capsid (3, ...concatemeric DNA and ... See full document
10
Herpes simplex virus type 1 DNA cleavage and encapsidation require the product of the UL28 gene: isolation and characterization of two UL28 deletion mutants.
... To demonstrate that the two UL28 mutants contain the deletion/insertion mutations at the appropriate position in the viral genome, we isolated total-cell DNA from Vero cells infected wit[r] ... See full document
11
Identification and Characterization of the UL56 Gene Product of Herpes Simplex Virus Type 2
... UL56 gene product of herpes simplex virus (HSV) has been shown to play an important role in viral ...The gene product was identified as three polypeptides with apparent ... See full document
11
Role of the UL25 Protein in Herpes Simplex Virus DNA Encapsidation
... are required for DNA cleavage and ...of DNA-containing C capsids on the capsid ...protein required: (i) to stabilize capsids after DNA is packaged; (ii) to trigger nuclear egress of the ... See full document
11
Herpes simplex virus type 1 gene products required for DNA replication: identification and overexpression.
... These data are consistent with the view that the genes ULS, UL8, UL9, UL42, and ULS2, like the genes encoding ICP8 and polymerase 12, 15, are all delayed-early genes, as expected for our[r] ... See full document
9
Encapsidation and expression of the herpes thymidine kinase gene in polyoma virus.
... At the indicated times, 5 ml of viral supernatant extracts were prepared, and 0.1 ml was assayed for A the ability to produce at 38°C viral T-antigen by extracts of PY60TK- plus TS25B-co[r] ... See full document
9
Disulfide Bond Formation in the Herpes Simplex Virus 1 UL6 Protein Is Required for Portal Ring Formation and Genome Encapsidation
... this region were unable to form rings; instead, L429E L436E and D-LZ formed polymorphic aggregates. In the context of viral infection, leucine zipper mutants as well as C166A and C254A assemble B capsids that contain ... See full document
9
Identification of a common antigen of herpes simplex virus bovine herpes mammillitis virus, and B virus.
... Of the additional polypep- to BHM virus per cm2; E 15 ,ul of [35SJmethioninetides present in the individual antigens Table labeled HSV-2 antigen precipitated in gel containing 1, the pol[r] ... See full document
6
An ICP6::lacZ insertional mutagen is used to demonstrate that the UL52 gene of herpes simplex virus type 1 is required for virus growth and DNA synthesis.
... To isolate and propagate this mutant, we generated a cell line, BL-1, by cotransfection of Vero cells with pSV2neo and a plasmid containing the herpes simplex virus type 1 KOS strain Bam[r] ... See full document
8
Characterization of the DNA-binding properties of herpes simplex virus regulatory protein ICP4.
... DNAbinding immunoassays with fraction VIII and portions of the HSV genome revealed stable complexes that contained ICP4 and specific sequences in the promoters for viral genes encoding I[r] ... See full document
9
Identification and characterization of a DNA primase activity present in herpes simplex virus type 1-infected HeLa cells.
... Percentage of DNA primase activity remaining in solution after treatment with anti-calf peptide IgGs and after binding of the antibody-antigen complex to protein A-Sepharose" Enzyme % Ac[r] ... See full document
8
Proteolytic Cleavage of VP1-2 Is Required for Release of Herpes Simplex Virus 1 DNA into the Nucleus
... total DNA) or 200 g DNase I/ml (90% protein, ⱖ2,000 U/mg protein; Sigma-Aldrich, ...encapsidated DNA) was added and samples incubated for 30 min at ...37°C. DNA was isolated by phenol-chloroform ... See full document
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