[PDF] Top 20 Identification of the Hepatitis C Virus E2 Glycoprotein Binding Site on the Large Extracellular Loop of CD81
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Identification of the Hepatitis C Virus E2 Glycoprotein Binding Site on the Large Extracellular Loop of CD81
... Hepatitis C virus (HCV) encodes a ⬃3,300-amino-acid poly- protein from which the E1 (polyprotein residues 191 to 383) and E2 (residues 384 to 746) glycoproteins are cleaved cotrans- ...and ... See full document
5
Identification of Amino Acid Residues in CD81 Critical for Interaction with Hepatitis C Virus Envelope Glycoprotein E2
... Human CD81 has been previously identified as the putative receptor for the hepatitis C virus envelope glycoprotein ...The large extracellular loop (LEL) of human ... See full document
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Human Monoclonal Antibodies That Inhibit Binding of Hepatitis C Virus E2 Protein to CD81 and Recognize Conserved Conformational Epitopes
... and identification of ...Epstein-Barr virus (EBV)-activated B cells to heteromyeloma cells produced human hybridomas, using methods as described elsewhere ...or E2 protein detected HCV- specific ... See full document
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Binding of the Hepatitis C Virus E2 Glycoprotein to CD81 Is Strain Specific and Is Modulated by a Complex Interplay between Hypervariable Regions 1 and 2
... single binding site only if a head-to-tail dimer is postulated, and this was indeed one of the conclusions derived from our model (44), which was once again indirectly supported by the SFV E1 ...the ... See full document
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An Optimized Hepatitis C Virus E2 Glycoprotein Core Adopts a Functional Homodimer That Efficiently Blocks Virus Entry
... the CD81-binding site when coupled with deletion of ...optimal CD81-LEL binding site configuration in the absence of the variable loops is predicted to favor a protective response ... See full document
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Characterization of Hepatitis C Virus E2 Glycoprotein Interaction with a Putative Cellular Receptor, CD81
... of E2 with the cell surface. E2 did not interact with African green monkey (AGM) CD81 on the surface of COS cells, which differs from the hCD81 sequence at four residues within the second ... See full document
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Identification of Conserved Residues in Hepatitis C Virus Envelope Glycoprotein E2 That Modulate Virus Dependence on CD81 and SRB1 Entry Factors
... and extracellular infectivities were quantified at several times ...affect virus replication, since the levels of expression of E1, E2, and NS5A were very similar for mutant and wild-type ...and ... See full document
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Conformational Flexibility in the Immunoglobulin Like Domain of the Hepatitis C Virus Glycoprotein E2
... the CD81 binding site within the HCV glycoprotein E2, the CD81 binding loop, adopts more than one distinct confor- FIG 5 More than one conformation of the Ig-like ... See full document
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Identification of a Residue in Hepatitis C Virus E2 Glycoprotein That Determines Scavenger Receptor BI and CD81 Receptor Dependency and Sensitivity to Neutralizing Antibodies
... mutant virus demonstrated a 10-fold increase in the sen- sitivity to neutralization by hCD81 LEL, suggesting an in- creased affinity of the gps for CD81 ...increased binding to CHO-CD81 cells ... See full document
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Role of Conserved E2 Residue W420 in Receptor Binding and Hepatitis C Virus Infection
... molecules, CD81 and SR-BI, have been reported to interact directly with the HCV envelope glyco- proteins (19, ...the binding of E2 through an interaction that involves hypervariable region 1 (HVR1), ... See full document
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Role of Conserved Cysteine Residues in Hepatitis C Virus Glycoprotein E2 Folding and Function
... in glycoprotein E2 have diverse effects on E2 folding and function in ...containing E2 glycoproteins with individual cysteine-to-alanine substitution ...by E2 glycoproteins containing ... See full document
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Identification of New Functional Regions in Hepatitis C Virus Envelope Glycoprotein E2
... and E2 as observed in the CD81 pull-down ...by CD81 indicated that E1 formed di- sulfide bond-linked high-molecular-weight complexes with these chimeric E2 proteins (data not ...and E2 ... See full document
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Functional Characterization of Intracellular and Secreted Forms of a Truncated Hepatitis C Virus E2 Glycoprotein
... The E2-specific conformation-dependent MAbs (H53, H35, and H54) were generated as previously reported ...human CD81 (hCD81)-transfected RBL (RBL-hCD81) cells, described previously (12), were propagated in ... See full document
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Hepatitis C Virus Hypervariable Region 1 Modulates Receptor Interactions, Conceals the CD81 Binding Site, and Protects Conserved Neutralizing Epitopes
... high virus titers in tissue culture (40), and compared RNA replica- tion, virus production, and infectivity of released particles be- tween Jc1 and a Jc1 mutant lacking HVR1, ...reporter virus ... See full document
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A Conserved Gly436-Trp-Leu-Ala-Gly-Leu-Phe-Tyr Motif in Hepatitis C Virus Glycoprotein E2 Is a Determinant of CD81 Binding and Viral Entry
... the CD81 LEL ...associated E2 was assessed by immunoprecipitation of radiolabeled E1E2-pps using MAb ...of E2 and p24 incorporated into E1E2-pp was similar in all cases ...E1E2-pp-derived E2 ... See full document
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Diverse CD81 Proteins Support Hepatitis C Virus Infection
... Hepatitis C virus (HCV) entry is dependent on ...the CD81 sequence is a determinant of HCV host range, we expressed a panel of diverse CD81 proteins and tested their ability to interact ... See full document
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Identification of Conserved Residues in the E2 Envelope Glycoprotein of the Hepatitis C Virus That Are Critical for CD81 Binding
... CD4 binding (37), resi- dues whose involvement was later confirmed when the crystal structure of gp120 was obtained ...in CD81 binding may exhibit amino acid hetero- geneity that is contributed by ... See full document
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Hepatitis C Virus Envelope Glycoprotein E2 Glycans Modulate Entry, CD81 Binding, and Neutralization
... of E2 glycan re- moval on antibody ...of E2, indicating that this do- main is exposed on the virion ...the CD81 binding site, rendering it more accessible to CD81 as well as ... See full document
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Binding of Hepatitis C Virus E2 Glycoprotein to CD81 Does Not Correlate with Species Permissiveness to Infection
... Hepatitis C virus (HCV) glycoprotein E2 binds to human cells by interacting with the CD81 molecule, which has been proposed to be the viral ...between binding to ... See full document
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A View of the E2-CD81 Interface at the Binding Site of a Neutralizing Antibody against Hepatitis C Virus
... the E2 protein was cloned into the mammalian cell expression vector pFUSE-Fc2-IL2ss (Invivogen, San Diego, CA) to produce an IgG1 Fc-fused E2 protein, referred to as ...the CD81-Fc construct was ... See full document
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