[PDF] Top 20 Identification of Mycobacterium tuberculosis Antigens of High Serodiagnostic Value
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Identification of Mycobacterium tuberculosis Antigens of High Serodiagnostic Value
... Antigen identification, cloning, and purification. M. tuberculosis genes were selected as previously described ...M. tuberculosis genes included those previously identified by serological expression ... See full document
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Identification of Mycobacterium tuberculosis antigens in Seibert fractions by immunoblotting
... Antibody activity was observed primarily with antigens in the polysaccharide Il and A protein fractions; these fractions were further evaluated by immunoblotting with sera from individua[r] ... See full document
5
Mycobacterium tuberculosis antigens MPT63 and MPT83 increase phagocytic activity of murine peritoneal macrophages
... The value of full-size antigen in comparison with its short fragment FLD rMPT83(115-220) obtained previously in our lab shows its identification in the soluble fraction, which indicates the correct folding ... See full document
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Cloning and Expression of Immunoreactive Antigens from Mycobacterium tuberculosis
... TB antigens, with the respective gene sizes, theoretical molecular masses, and pI values is shown in Table ...these antigens using the commercial anti-RGS His ...were high for the ...purified ... See full document
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Identification of Novel Seroreactive Antigens in Johne's Disease Cattle by Using the Mycobacterium tuberculosis Protein Array
... hominissuis, Mycobacterium intracellulare, Mycobacterium smegma- tis, and Mycobacterium ...enable high-density printing and strong protein attachment via covalent cross-linking and ... See full document
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Assessment of Five Antigens from Mycobacterium tuberculosis for Serodiagnosis of Tuberculosis
... those antigens that can distinguish active TB from LTBI and improve the sensi- tivity and specificity of the TB diagnosis (8, ...TB identification (2), is the most widely studied ...M. tuberculosis ... See full document
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Antibody Responses to Mycobacterial Antigens in Children with Tuberculosis: Challenges and Potential Diagnostic Value
... mycobacterial antigens can distinguish be- tween LTBI and TB (reviewed in reference ...mycobacterial antigens for potential serodiagnostic ...mycobacterial antigens have been evaluated for the ... See full document
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Novel Secreted Antigens of Mycobacterium paratuberculosis as Serodiagnostic Biomarkers for Johne's Disease in Cattle
... To test the potential of F15 proteins as diagnostic reagents, we separated the cow sera (n ⫽ 25) into 4 groups based on the matched IDEXX ELISA reading and M. avium subsp. paratuber- culosis CFU per gram of feces (see ... See full document
9
Serodiagnostic Potential of Culture Filtrate Antigens of Mycobacterium tuberculosis
... for tuberculosis (TB) have been made by research- ers at several laboratories (7, ...M. tuberculosis, which provides very high specificity (⬎98%) (3, ...(28). Antigens (Ag) 85A and B have also ... See full document
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Serum Antibody Responses to 10 Mycobacterium tuberculosis Proteins, Purified Protein Derivative, and Old Tuberculin in Natural and Experimental Tuberculosis in Rhesus Monkeys
... of tuberculosis (TB) but often yield poor specificity and anergy in ...recombinant antigens, PPD, and OT in rhesus monkeys experimentally infected with Mycobacterium tuberculosis and analyzed ... See full document
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Assessing the Serodiagnostic Potential of 35 Mycobacterium tuberculosis Proteins and Identification of Four Novel Serological Antigens
... had high titers of antibody toward ST-CF, which is a com- plex mixture of proteins from ...the antigens could be divided into three different categories, as illustrated in ...the antigens (category ... See full document
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Antigens of Mycobacterium tuberculosis Recognized by Antibodies during Incipient, Subclinical Tuberculosis
... FIG. 1. Reactivity of fractionated CFPs with pooled serum samples from healthy, PPD skin test-positive individuals (A) and subclinical TB serum samples from HIV ⫹ TB ⫹ individuals (B). Antigens recognized by both ... See full document
5
PCR Based Method To Differentiate the Subspecies of the Mycobacterium tuberculosis Complex on the Basis of Genomic Deletions
... PCR amplification primers and conditions. The target gene loci and their primer names, primer sequences, primer locations in the M. tuberculosis H37Rv genome (accession no. NC_000962) (9), and amplification ... See full document
14
Clinical Evaluation of TRCRapid M TB for Detection of Mycobacterium tuberculosis Complex in Respiratory and Nonrespiratory Specimens
... Patients and clinical specimens. This study was approved by the Yokohama City University Medical Center Ethics Committee. From December 2006 to November 2008, specimens were obtained from 1,282 patients, including chil- ... See full document
6
Novel polyhydroxyalkanoate beads for use as a vaccine against tuberculosis : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Microbiology at Massey University, Manawatū, New Zealand
... form of transmission. New vaccine candidates are being developed to provide protection against tuberculosis. Subunit vaccines offer a safer alternative than whole cell preparations and provide the possibility of ... See full document
13
Rapid Differentiation of Mycobacterium tuberculosis and M bovis by High Resolution Melt Curve Analysis
... M. tuberculosis and ...of high-resolution melt curve analysis (HRM) to differentiate between ...M. tuberculosis by two stepwise reac- ...M. tuberculosis and all other members of the MTC ...M. ... See full document
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Monoclonal antibodies to surface antigens of Mycobacterium tuberculosis and their use in a modified enzyme linked immunosorbent spot assay for detection of mycobacteria
... cross-reactive, high- affinity MAb (MAb 5c11) with a more selective MAb (MAb 9d8) has the potential for offering sensitivity and specificity when analyzing clinical ... See full document
8
Design and Construction of a Cloning Vector Containing the hspX Gene of Mycobacterium tuberculosis
... Cerebral spinal fluid samples from 555 TBM- positive children (M. tuberculosis-culture positive, n = 29) were collected from three centers in Delhi. In this test, TBM (true positives) and NTIM (true negative) ... See full document
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Comparative Evaluation of the New Version of the INNO LiPA Mycobacteria and GenoType Mycobacterium Assays for Identification of Mycobacterium Species from MB/BacT Liquid Cultures Artificially Inoculated with Mycobacterial Strains
... GenoType Mycobacterium (Hain Diagnostika, Nehren, Germany), were evaluated for identification of mycobacterial species isolated from liquid ...simultaneous identification of different mycobacterial ... See full document
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Novel Multiplex Real Time PCR Diagnostic Assay for Identification and Differentiation of Mycobacterium tuberculosis, Mycobacterium canettii, and Mycobacterium tuberculosis Complex Strains
... Tuberculosis (TB) is the leading cause of death worldwide from an infectious agent (13), with the WHO estimating that one-third of the global population is infected with Mycobacte- rium tuberculosis. In a ... See full document
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