[PDF] Top 20 Identification of Staphylococcus spp by PCR Restriction Fragment Length Polymorphism Analysis of dnaJ Gene
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Identification of Staphylococcus spp by PCR Restriction Fragment Length Polymorphism Analysis of dnaJ Gene
... reference Staphylococcus species and subspecies were selected from the Polish Collection of Microorganisms, the Czech Collection of Micro- organisms, and the American Type Culture ... See full document
5
Rapid identification of mycobacteria to species level by PCR restriction fragment length polymorphism analysis of the hsp65 gene and proposition of an algorithm to differentiate 34 mycobacterial species
... Classical identification results for mycobacteria based on cultural and biochemical tests may take several weeks after reception of specimens, and the tests sometimes fail to produce a precise ...and gene ... See full document
5
Rapid identification of Campylobacter species by restriction fragment length polymorphism analysis of a PCR amplified fragment of the gene coding for 16S rRNA
... PCR-RFLP. (i) Bacterial extracts. Strains were stored at 2 70 8 C in tryptic soy broth containing 20% (vol/vol) glycerol. Bacteria were grown for 48 h at 36 8 C on heart infusion agar or Mueller-Hinton agar ... See full document
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Identification of 54 Mycobacterial Species by PCR Restriction Fragment Length Polymorphism Analysis of the hsp65Gene
... for restriction fragment length polymorphism of a PCR-amplified 439-bp segment of the gene encoding the 65-kDa heat shock ...protein. Restriction digests were separated by ... See full document
8
Molecular Typing of Nocardia Species
... Conventional identification of Nocardia species in routine medical laboratories which is based on phenotypic (cellular morphology, colonial characteristics), biochemical and enzymatic profiles, and chemotaxonomic ... See full document
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Rapid Identification and Typing ofStaphylococcus aureus by PCR Restriction Fragment Length Polymorphism Analysis of the aroA Gene
... Staphylococcus aureus is the causative agent of many oppor- tunistic infections in humans and animals (8). As a human pathogen, S. aureus causes superficial, deep-skin, and soft- tissue infections, endocarditis, ... See full document
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Molecular Typing of Staphylococcus aureus Based on PCR Restriction Fragment Length Polymorphism and DNA Sequence Analysis of the Coagulase Gene
... for Staphylococcus aureus was developed based on improved PCR amplification of the coagulase gene and restriction fragment length polymorphism (RFLP) analysis of ... See full document
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Molecular Identification of Capnocytophaga spp via 16S rRNA PCR Restriction Fragment Length Polymorphism Analysis
... rRNA PCR-RFLP and 16S rRNA gene sequencing, were per- formed blindly so as to avoid any bias in interpretation of the ...rRNA gene sequencing and (ii) due to low RDP-II similarity values in spite of ... See full document
8
Genetic Identification of the Main Opportunistic Mucorales by PCR Restriction Fragment Length Polymorphism
... bioinformatic analysis was necessary to design the best strategy to ...the PCR and avoided the amplification of other fungal and human ...the PCR on several strains of Mucorales, ... See full document
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Identification of Oral Peptostreptococcus Isolates by PCR Restriction Fragment Length Polymorphism Analysis of 16S rRNA Genes
... start” PCR was used, whereby the primers are separated from other reaction com- ponents by a layer of wax (DyNAwax; Flowgen), therefore preventing the reaction from starting until the wax has melted upon ... See full document
5
Routine use of PCR restriction fragment length polymorphism analysis for identification of mycobacteria growing in liquid media
... original PCR- RFLP study were not recognized by the algorithm ...the fragment size variations described above, the novel restriction patterns of reference cultures and patient isolates have been ... See full document
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Detection of Cryptosporidium and Identification to the Species Level by Nested PCR and Restriction Fragment Length Polymorphism
... for PCR or kept at ⫺ 20°C. Bioinformatics. Preliminary comparative analysis of the TRAP-C1, COWP, HSP70, and 18S rRNA gene sequences resulted in the selection of the Crypto- sporidium 18S rRNA ... See full document
7
Identification of Infectious Agents in Onychomycoses by PCR Terminal Restriction Fragment Length Polymorphism
... TRFLP analysis did not correspond to any of the peaks in the reference ladder (Table 2 and ...unique identification was obtained in 12 cases (Table ... See full document
9
Evaluation of PCR Restriction Profile Analysis and IS2404 Restriction Fragment Length Polymorphism and Amplified Fragment Length Polymorphism Fingerprinting for Identification and Typing of Mycobacterium ulcerans andM marinum
... In conclusion, the present study demonstrates the limita- tions of the 16S rRNA-based PRPA technique to differentiate M. ulcerans from M. marinum and the usefulness of the DNA fingerprinting techniques utilizing IS2404 ... See full document
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Identification of Abiotrophia adiacens and Abiotrophia defectiva by 16S rRNA gene PCR and restriction fragment length polymorphism analysis
... Preparation of genomic DNA for PCR analysis. (i) DNA preparation. Bacte- rial cells from a 1.5-ml culture were harvested, washed with ice-cold TE (50 mM Tris-HCl [pH 8.0], 1 mM EDTA), and then suspended in ... See full document
6
PCR Restriction Fragment Length Polymorphism Analysis for Identification of Bacteroides spp and Characterization of Nitroimidazole Resistance Genes
... rDNA PCR-RFLP identification. UPGMA dendro- grams of the 16S rDNA PCR restriction profiles obtained for type strains of Bacteroides ...unequivocal identification of 65 (93%) of the 70 ... See full document
5
Phylogenetic Analysis and PCR Restriction Fragment Length Polymorphism Identification of Campylobacter Species Based on Partial groEL Gene Sequences
... the identification of Campy- lobacter species according to phenotypic properties may result in false identifications, as many species include strains that give atypical results in some key phenotypic tests (24, ... See full document
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Species Identification of Mycobacteria by PCR Restriction Fragment Length Polymorphism of therpoB Gene
... The restriction analysis of a 360-bp fragment within this gene after single MspI digestion is highly effective for differ- entiating most mycobacteria even at the species ... See full document
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Diversity of Staphylococcus Species Strains Based on Partial kat (Catalase) Gene Sequences and Design of a PCR Restriction Fragment Length Polymorphism Assay for Identification and Differentiation of Coagulase Positive Species (S aureus, S delphini, S hyicus, S intermedius, S pseudintermedius, and S schleiferi subsp coagulans)
... degenerate PCR primers was designed and used to amplify and sequence about 75% of the catalase (kat) gene from each of 49 staphylococcal ...of Staphylococcus xylosus, ...kat gene sequences ... See full document
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hsp65 Sequencing for Identification of Rapidly Growing Mycobacteria
... Partial sequencing of the hsp65 gene was used for the identification of rapidly growing mycobacteria (RGM). A 441-bp fragment (A. Telenti, F. Marchesi, M. Balz, F. Bally, E. Bo¨ttger, and T. Bodmer, ... See full document
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