[PDF] Top 20 Rapid Identification and Typing ofStaphylococcus aureus by PCR Restriction Fragment Length Polymorphism Analysis of the aroA Gene
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Rapid Identification and Typing ofStaphylococcus aureus by PCR Restriction Fragment Length Polymorphism Analysis of the aroA Gene
... Staphylococcus aureus is the causative agent of many oppor- tunistic infections in humans and animals ...S. aureus causes superficial, deep-skin, and soft- tissue infections, endocarditis, and bacteremia, ... See full document
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Rapid identification of Campylobacter species by restriction fragment length polymorphism analysis of a PCR amplified fragment of the gene coding for 16S rRNA
... The PCR-RFLP method described here differentiates be- tween the related genera Campylobacter, Arcobacter, Helico- bacter, and ...level. PCR amplification generated an amplicon of 283 bp from all isolates ... See full document
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Identification of Staphylococcus spp by PCR Restriction Fragment Length Polymorphism Analysis of dnaJ Gene
... Staphylococcus aureus is clinically the most signif- icant Staphylococcus species, as it causes many types of infec- tions, other coagulase-negative staphylococci (CoNS) are in- creasingly becoming recognized as ... See full document
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Evaluation of PCR Restriction Profile Analysis and IS2404 Restriction Fragment Length Polymorphism and Amplified Fragment Length Polymorphism Fingerprinting for Identification and Typing of Mycobacterium ulcerans andM marinum
... genetic analysis of ...tion fragment polymorphism analysis, strongly supports this hypothesis ...the identification of ...The PCR-based AFLP tech- nique is such a whole-genome ... See full document
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Rapid Identification of Bacteria from Positive Blood Cultures by Terminal Restriction Fragment Length Polymorphism Profile Analysis of the 16S rRNA Gene
... the PCR amplification efficiency. One distinct advantage of our data analysis program is the ability to incorporate a search for the profile of a secondary organism, thereby auto- matically determining the ... See full document
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Diversity of Staphylococcus Species Strains Based on Partial kat (Catalase) Gene Sequences and Design of a PCR Restriction Fragment Length Polymorphism Assay for Identification and Differentiation of Coagulase Positive Species (S aureus, S delphini, S hyicus, S intermedius, S pseudintermedius, and S schleiferi subsp coagulans)
... degenerate PCR primers was designed and used to amplify and sequence about 75% of the catalase (kat) gene from each of 49 staphylococcal ...kat gene sequences were less conserved than those of 16S ... See full document
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Molecular typing of Borrelia burgdorferi sensu lato by PCR restriction fragment length polymorphism analysis
... Elucidation of the relationship between species and disease is complicated by the difficulty in culturing B. burgdorferi sensu lato. While purified isolates have been obtained from a variety of biologic sources ... See full document
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Molecular Typing of Nocardia Species
... Conventional identification of Nocardia species in routine medical laboratories which is based on phenotypic (cellular morphology, colonial characteristics), biochemical and enzymatic profiles, and chemotaxonomic ... See full document
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Identification of 54 Mycobacterial Species by PCR Restriction Fragment Length Polymorphism Analysis of the hsp65Gene
... for restriction fragment length polymorphism of a PCR-amplified 439-bp segment of the gene encoding the 65-kDa heat shock ...protein. Restriction digests were separated by ... See full document
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Comparative Fingerprinting Analysis ofCampylobacter jejuni subsp jejuni Strains by Amplified Fragment Length Polymorphism Genotyping
... Amplified-fragment length polymorphism (AFLP) analysis with the endonucleases BglII and MfeI was used to genotype 91 Campylobacter jejuni ...DNA-based typing methods, pulsed-field gel ... See full document
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Identification of Abiotrophia adiacens and Abiotrophia defectiva by 16S rRNA gene PCR and restriction fragment length polymorphism analysis
... the PCR products were then exam- ined following digestion with the appropriate restriction en- zyme ...tional analysis by MspI digestion could afford the reliable iden- ...the restriction ... See full document
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PCR Restriction Fragment Length Polymorphism for Rapid, Low Cost Identification of Isoniazid Resistant Mycobacterium tuberculosis
... a rapid, simple PCR-RFLP screening test for the identification of isoniazid resistance from positive ...katG_315 analysis, with katG_S315T be- ing by far the most prevalent mutation (71%) ...A ... See full document
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Genotypic Analysis of Staphylococcus aureus Coagulase Gene using PCR-RFLP analysis
... Staphylococcus aureus is the second most important cause of foodborne diseases (1), which can be transmitted through milk and dairy products ...S. aureus infections, possible source(s) of infections should ... See full document
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Rapid identification of mycobacteria to species level by PCR restriction fragment length polymorphism analysis of the hsp65 gene and proposition of an algorithm to differentiate 34 mycobacterial species
... hsp65 gene present in all mycobacteria and in some other bacteria such as Nocardia ...The restriction anal- ysis of a 439-bp fragment within this gene after BstEII and HaeIII digestions is ... See full document
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Molecular Typing of Staphylococcus aureus Based on PCR Restriction Fragment Length Polymorphism and DNA Sequence Analysis of the Coagulase Gene
... S. aureus 1 (EMRSA-1) was the principal MRSA strain identified by phage typing in England ...phage typing to our staphylococcus refer- ence service ...alternative rapid and cost-effective ... See full document
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Species Identification of Mycobacteria by PCR Restriction Fragment Length Polymorphism of therpoB Gene
... Currently, identification of clinical isolates of mycobacteria to the species level is primarily based on cultural characteristics and biochemical ...sequence analysis (3, 4, 15–17, 19, 26, 31, 32), can ... See full document
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Evaluation of Mycobacterial Interspersed Repetitive Unit Variable Number Tandem Repeat Analysis and Spoligotyping for Genotyping of Mycobacterium bovis Isolates and a Comparison with Restriction Fragment Length Polymorphism Typing
... strain typing methods for differentiation of Mycobacterium bovis isolates include restriction endonuclease analysis (REA), restriction fragment length polymorphism (RFLP) ... See full document
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hsp65 Sequencing for Identification of Rapidly Growing Mycobacteria
... target gene must be sufficiently conserved among the strains of the species for use in genotypic ...the identification of the main RGM species affecting humans by studying a representative panel of strains ... See full document
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Phylogenetic Analysis and PCR Restriction Fragment Length Polymorphism Identification of Campylobacter Species Based on Partial groEL Gene Sequences
... to PCR-RFLP analy- sis of the 23S rRNA gene and dot blot ...a PCR product for the hippuricase gene ...hippuricase PCR product smaller than expected have also been reported (6, ... See full document
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Differentiation of Borrelia burgdorferiSensu Lato on the Basis of RNA Polymerase Gene (rpoB) Sequences
... rDNA analysis (23). Also, the MseI and DraI restriction patterns of the 5S-23S intergenic spacer amplicons of these strains differed from those of the other ... See full document
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