[PDF] Top 20 Imaging of the Alphavirus Capsid Protein during Virus Replication
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Imaging of the Alphavirus Capsid Protein during Virus Replication
... Tagged capsid protein could be fluorescently labeled with biarsenical dyes in living cells without effects on virus growth, mor- phology, or protein ...cell imaging and colocalization ... See full document
11
Alphavirus Capsid Protein Helix I Controls a Checkpoint in Nucleocapsid Core Assembly
... in virus replication and a defect in core formation in the ...a virus with a wild-type phenotype, and this chimeric virus accumu- lated cores in the ... See full document
8
Formation of nsP3-Specific Protein Complexes during Sindbis Virus Replication
... For confocal microscopy, BHK-21 cells were seeded on the glass chamber slides and infected at an MOI of 20 PFU/cell or 20 IFU/cell with recombinant SIN or packaged SIN replicon, respectively, and incubated at 37°C in a ... See full document
13
Suppression of Leishmania RNA virus replication by capsid protein overexpression.
... that replication of LRV can be ...of virus occurred in the clones derived from the 5-ASKH over- expressing the capsid ...of virus in the clones derived from this line suggests that an LRV 2 ... See full document
5
Interactions between Rubella Virus Capsid and Host Protein p32 Are Important for Virus Replication
... affected during infection with rubella virus (RV). Expression of the capsid, in the absence of other viral proteins, was found to induce both perinuclear clustering of mitochondria and the formation ... See full document
14
Protein Phosphatase 1α Interacts with Venezuelan Equine Encephalitis Virus Capsid Protein and Regulates Viral Replication through Modulation of Capsid Phosphorylation
... VEEV replication, as signif- icant inhibition of viral titers was observed beginning at 4 hpi and continuing up to at least 24 ...viral protein production, extracellular viral RNA, and intracellular viral ... See full document
18
Multiple roles of the non-structural protein 3 (nsP3) alphavirus unique domain (AUD) during Chikungunya virus genome replication and transcription
... of replication may have resulted in the P247A/ V248A values rising above the threshold for ...detection. Virus data in C2C12 cells were consis- tent with replicon results: following electroporation of viral ... See full document
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Expression of the Zinc-Finger Antiviral Protein Inhibits Alphavirus Replication
... SIN replication by preventing the accumulation of new genomic viral RNA in the ...SIN replication cycles compared to ...Toto1101 virus and luciferase-expressing ...structural protein ... See full document
8
Analyses of Phosphorylation Events in the Rubella Virus Capsid Protein: Role in Early Replication Events
... of capsid phosphorylation in virus ...the capsid is important for virus replication, assembly of virions is not dependent upon this ...early replication defects, possibly due to ... See full document
9
Leader of the Capsid Protein in Feline Calicivirus Promotes Replication of Norwalk Virus in Cell Culture
... the replication of NV in cells. During the generation of NV replicon-bearing cells with pNV-Neo in our previous study, we noticed that only a few populations of cells (either BHK21 or Huh-7) could support ... See full document
12
Functions of Varicella-Zoster Virus ORF23 Capsid Protein in Viral Replication and the Pathogenesis of Skin Infection
... major capsid protein, ...VZV capsid assembly in which nuclear transport of the major capsid protein and associated proteins requires ORF23 during VZV replication in the ... See full document
16
Alphavirus polymerase and RNA replication
... for replication purposes are studied by several groups but clearly much is still to be found 542 (reviewed in (Miller and Krijnse-Locker, 2008; Reid et ...nonstructural protein interactions and their 544 ... See full document
43
An Antiviral Role for Antimicrobial Peptides during the Arthropod Response to Alphavirus Replication
... to Alphavirus Replication Zhijing Huang,* Megan ...the virus to vertebrate ...an alphavirus replicon and con- trol green fluorescent protein flies identified 95 SINrep-sensitive ... See full document
10
Cooperative Effect of Gag Proteins p12 and Capsid during Early Events of Murine Leukemia Virus Replication
... Strikingly, the MLV preintegration complexes isolated from Fv-1-restricted cells were also functional in vitro, again similar to the p12 mutant (32). One intriguing hypothesis is that both p12 and CA are im- portant in ... See full document
11
Functional Replacement of a Domain in the Rubella Virus P150 Replicase Protein by the Virus Capsid Protein
... that virus with the NotI deletion was viable in cell culture due to the presence of C encoded by the SP-ORF ...a virus in which C(1-277) was inserted between the NotI sites in P150 (corresponding to the ... See full document
7
Imaging the Alphavirus Exit Pathway
... of alphavirus assembly and budding are poorly ...fluorescent protein labels on the E2 envelope protein and exploited them to characterize virus as- sembly and budding in living ...cells. ... See full document
12
Assembly of Alphavirus Replication Complexes from RNA and Protein Components in a Novel trans-Replication System in Mammalian Cells
... RNA virus enters the cytoplasm of a permissive cell, it is first ...to replication complexes, where RNA replication commences with the synthesis of at least one com- plementary negative strand, which ... See full document
13
Ultrastructural Study of Rotavirus Replication and Localization of the Intermediate Capsid Protein VP6
... layered protein capsid [1, 2]. During viral maturation, double layered particles (previously known as single–shelled particles) assembled from VPI, VP2, VP3 and VP6 in the cytoplasm, interact with ... See full document
5
Phosphorylation of Rubella Virus Capsid Regulates Its RNA Binding Activity and Virus Replication
... RV capsid at the appropri- ate time and ...that capsid phosphorylation is dynamic, a situation that could facilitate efficient binding of viral RNA and, just as importantly, release of RNA at the ... See full document
8
Capsid-CPSF6 Interaction Is Dispensable for HIV-1 Replication in Primary Cells but Is Selected during Virus Passage In Vivo
... HIV-1 capsid (CA) protein and has been implicated in nuclear entry and integration ...cal during HIV-1 replication in ...HIV-1 capsid mutant that reduces binding to CPSF6, is largely ... See full document
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