[PDF] Top 20 Inducible gene expression system by 3-hydroxypropionic acid
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Inducible gene expression system by 3-hydroxypropionic acid
... of 3‑HP‑inducible promoters in P. denitrificans 3-Hydroxypropionic acid (3-HP) is a carbon compound not commonly encountered in the natural environment; neither its use as a ... See full document
8
Fenino, Elena (2010): Genetic Dissection of Organellar-Translation-Dependent Retrograde Signalling in Arabidopsis. Dissertation, LMU München: Fakultät für Biologie
... the system peculiarity to respond in a dose-dependent ...our inducible lines did not show any basal expression level when grown in vitro: if not exposed to ethanol vapour, pAlc::MRPL11 mrpl11 line, ... See full document
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A Rhamnose-Inducible System for Precise and Temporal Control of Gene Expression in Cyanobacteria
... YFP expression from the rhaBAD promoter in Synechocystis, the fl uorescence intensity of cells sampled from in the same transformant cultures was monitored over a longer period (Figure ...of gene ... See full document
12
Anodic electro-fermentation of 3-hydroxypropionic acid from glycerol by recombinant Klebsiella pneumoniae L17 in a bioelectrochemical system
... Both the anode and cathode electrodes were connected via a 1 kΩ external resistance or a potentiostat for closed circuit operation (BES operating condition), whereas in non-BES operation, the anode was not connected to ... See full document
11
Tetracycline-Inducible Gene Expression and Gene Deletion in Candida albicans
... -M2 gene contained in plasmid pUHrT62-1 (44) showed that in addition to the amino acid exchanges that created rtTA-M2 a total of 19 CTG codons, which would be mistranslated as serine instead of leucine in ... See full document
16
Enhancement of 3-hydroxypropionic acid production from glycerol by using a metabolic toggle switch
... To overcome this issue, there have recently been sev- eral attempts to control metabolic flux by modulating the expression levels of enzymes involved in the central metabolic pathway [10–13]. We previously ... See full document
14
Increased 3-hydroxypropionic acid production from glycerol, by modification of central metabolism in Escherichia coli
... [16-19]. To date, various studies have reported increased 3HP production [10-19]. For example, Rathnasingh et al. optimized the expression level of each enzyme in this pathway in E. coli [17]. Ashok et al. deleted ... See full document
11
Expanding the molecular toolbox for Lactococcus lactis: construction of an inducible thioredoxin gene fusion expression system
... hetero(multi)meric protein complexes, as certain pro- tein-protein interactions may be prevented. The base- plate (Bpp) of Tuc2009 is a large multimeric protein complex involved in host recognition [39-43]. It consists ... See full document
10
Gene Expression of Nucleic Acid-Sensing Pattern Recognition Receptors in Children Hospitalized for Respiratory Syncytial Virus-Associated Acute Bronchiolitis
... in acid nucleic recognition in the initiation of innate immunity and the orchestration of adaptive immunity, the aim of this study was to determine whether any heterogeneity of PRR expression in the airway ... See full document
8
Implementation of the agmatine-controlled expression system for inducible gene expression in Lactococcus lactis
... net expression would lie between 5 and 7 h after culturing ...NICE system (2–3 h after adding ...Table 3 Effect of agmatine on growth, μ max , pH and ... See full document
12
Retinoic acid inducible gene I Activates innate antiviral response against human parainfluenza virus type 3
... RIGI expression was confirmed by Western blotting (with FLAG antibody) of lysate obtained from RIGI-FLAG trans- fected 293 cells ...RIGI expression resulted in drastic activation (measured by luciferase ... See full document
7
Construction of a doxycycline inducible adipogenic lentiviral expression system
... aP2 expression in HIB-1B cells treated for 24 h with rosig- litazone and forskolin was increased by 65-fold (P < ...aP2 expression by around 4-fold (P < 0.05) (Fig. 5A). Since expression of ... See full document
8
Retinoic Acid-Inducible Gene I Mediates Early Antiviral Response and Toll-Like Receptor 3 Expression in Respiratory Syncytial Virus-Infected Airway Epithelial Cells
... TLR3 expression. We found that UV-RSV-CM induced TLR3 expression in naı¨ve A549 cells, indicating that paracrine activators of TLR3 expression were present in infected A549 cell culture ...TLR3 ... See full document
11
Engineering an efficient and tight d-amino acid-inducible gene expression system in Rhodosporidium/Rhodotorula species
... erase expression was observed when 70 mM d-alanine was used as the sole carbon and nitrogen ...the expression by ...the expression of luciferase although increasing the concentration further enhanced ... See full document
16
Characterisation of a 3 hydroxypropionic acid inducible system from Pseudomonas putida for orthogonal gene expression control in Escherichia coli and Cupriavidus necator
... As hypothesized, the concentration of D-3-HB (21) in the supernatant of the C. necator culture decreased rapidly (Fig. 4c). From the initial concentration of 5 mM D-3-HB (21), only 0.3 ± 0.1 mM remained in ... See full document
13
PubMedCentral-PMC4956550.pdf
... upregulate expression of cardiac genes, including sarcomere components Actc1 and Tnnt2 and ion channel subunits Slc8a1, Kcna5, and Scn5a ...Similarly, inducible MGT expression in primary MEFs turns ... See full document
13
Androgen receptor expression in human prostate cancer cell lines
... AR expression observed in DUSF (up- regulation) and LNCaP (down-regulation) cells has been reported to be transcriptional and cell line-specific (Dai and Bumstein, 1996; Dai et ...AR gene is ... See full document
230
Production of 3-hydroxypropionic acid in engineered Methylobacterium extorquens AM1 and its reassimilation through a reductive route
... LC-QQQ-MS system (Agilent 1290 Infinity-6460, Agilent Technologies, Santa Clara, CA, USA), an Agilent LC-QTOF (Agilent 1290 Infinity-6530B, Agilent Tech- nologies, Santa Clara, CA, USA) or a LC-QExactive-MS ... See full document
17
An inducible transgene expression system for zebrafish and chick
... the inducible GAL4 system, we chose to use a variant of GAL4 in which the DNA-binding domain is fused to two copies of a truncated VP16 activation domain (Asakawa et ...drive expression in a variety ... See full document
9
Development of a Genetic Toolbox for Geobacillus kaustophilus using Novel Bacteriophages GBK1 and GBK2.
... protein expression using promoters (both constitutive and inducible), ribosome binding site characterization, and protein secretion systems are also being explored but are ... See full document
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