[PDF] Top 20 De Novo Initiation Pocket Mutations Have Multiple Effects on Hepatitis C Virus RNA-Dependent RNA Polymerase Activities
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De Novo Initiation Pocket Mutations Have Multiple Effects on Hepatitis C Virus RNA-Dependent RNA Polymerase Activities
... hydrophobic C-terminal 21 amino acids. Six histidines were FIG. 1. Mutations within the catalytic pocket of the HCV RdRp and assays for activities of the HCV ...the mutations in the HCV ... See full document
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Hydrophobic and Charged Residues in the C-Terminal Arm of Hepatitis C Virus RNA-Dependent RNA Polymerase Regulate Initiation and Elongation
... When C-terminal domain mutants were tested in a sub- genomic replicon system, the respective RNAs showed marked decreases in translation and replication despite having increased RdRp activity in ...phenotypes ... See full document
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Selection of 3′-Template Bases and Initiating Nucleotides by Hepatitis C Virus NS5B RNA-Dependent RNA Polymerase
... the de novo poly- merase activity is template ...of RNA templates af- fects the ...the de novo polymerase activity: (i) interaction between the RNA template and ... See full document
10
Membrane Association of the RNA-Dependent RNA Polymerase Is Essential for Hepatitis C Virus RNA Replication
... by multiple silent mutations inter- fering with the predicted stem-loop structures ...silent mutations results in a complete loss of replication competence ...verse effects of the insertion ... See full document
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RNA Synthesis by the Brome Mosaic Virus RNA-Dependent RNA Polymerase in Human Cells Reveals Requirements for De Novo Initiation and Protein-Protein Interaction
... for polymerase activity and for interaction with ...were de- scribed in the work of Kao et ...(B) Effects of deletions in 2a on activity and the enhancement of polymerase activity by the WT ... See full document
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De Novo Initiation of RNA Synthesis by Hepatitis C Virus Nonstructural Protein 5B Polymerase
... De novo RNA synthesis using chemically synthesized small RNA as ...initiate de novo RNA synthesis, small RNAs (21 or 22 nt) representing the 3⬘-terminal sequence of HCV ... See full document
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Regulation of De Novo-Initiated RNA Synthesis in Hepatitis C Virus RNA-Dependent RNA Polymerase by Intermolecular Interactions
... rescue de novo initiation by the PV mutant ...in de novo FIG. 5. The E18R mutant can stimulate de novo initiation by ⌬ ...the de novo-initiated ... See full document
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De Novo Initiation of RNA Synthesis by the RNA-Dependent RNA Polymerase (NS5B) of Hepatitis C Virus
... the C-terminal 18 amino acids of NS5B have any effects on in vitro RdRp activity under the conditions used in this ...D(⫹) RNA as a template. It was found that the C-terminal ... See full document
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Mechanism of De Novo Initiation by the Hepatitis C Virus RNA-Dependent RNA Polymerase: Role of Divalent Metals
... novo initiation but also reduced primer extension ...the activities of viral RdRps in addition to performing nucleotide ...dengue virus replicase was suggested to exist in at least two ... See full document
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Mutagenesis Analysis of the rGTP-Specific Binding Site of Hepatitis C Virus RNA-Dependent RNA Polymerase
... acid mutations of the rGTP-specific binding site had any significant effect on the in vitro RNA synthesis catalyzed by purified NS5B ...The RNA products synthesized by HCV RdRp from the HCV ... See full document
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Characterization of two RNA polymerase activities induced by mouse hepatitis virus.
... As expected, the later the cycloheximide was added, the more total RNA was synthesized, suggesting that protein synthesis was required throughout the infection.. was less than that when [r] ... See full document
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Hepatitis C Virus RNA Replication Requires a Conserved Structural Motif within the Transmembrane Domain of the NS5B RNA-Dependent RNA Polymerase
... //mobyle.pasteur.fr/cgi-bin/MobylePortal/portal.py?form_toppred), and SOSUI (http://bp.nuap.nagoya-u.ac.jp/sosui/). T, predicted transmem- brane amino acid; #, consensus minimum transmembrane segment, considering all ... See full document
5
Transcription of Hepatitis Delta Virus RNA by RNA Polymerase II
... HDV RNA, we made a comparison between 293-HDV and 293- ␦ Ag ...HDV RNA. It could have been a direct interaction, as has been suggested by Handa and coworkers from in vitro studies (41) and yeast ... See full document
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Association of the Influenza A Virus RNA-Dependent RNA Polymerase with Cellular RNA Polymerase II
... influenza RNA polymerase were performed as described previously ...influenza virus polymerase copurified with TAP-CTD was used to transcribe a short (14-nucleotide) viral RNA template ... See full document
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Antiviral Effects of a Transgenic RNA-Dependent RNA Polymerase
... lethal virus-induced ...and C) encoded by the bicistronic P gene of the Indiana serotype of vesicular stomatitis virus and analysis of their effect on transcription by the viral RNA ... See full document
5
Interaction with a Ubiquitin-Like Protein Enhances the Ubiquitination and Degradation of Hepatitis C Virus RNA-Dependent RNA Polymerase
... of hepatitis C virus (HCV) RNA-dependent RNA polymerase (NS5B), we searched for cellular proteins interacting with NS5B protein by yeast two-hybrid screening of a human ... See full document
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RNA Replication without RNA-Dependent RNA Polymerase: Surprises from Hepatitis Delta Virus
... genomic RNA (considered a negative-strand RNA by virtue of the fact that the HDAg- coding sequence is present on the antigenomic strand) is then replicated into the antigenomic ...cycle have been ... See full document
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Different De Novo Initiation Strategies Are Used by Influenza Virus RNA Polymerase on Its cRNA and Viral RNA Promoters during Viral RNA Replication
... single-stranded RNA viruses to initiate and control their replication via the synthesis of replicative ...same virus-encoded polymerase is responsible for both genome and antigenome strand synthesis ... See full document
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Structural Analysis of the Hepatitis C Virus RNA Polymerase in Complex with Ribonucleotides
... ing that the second hydrogen bond is water mediated, as ex- pected since both the guanidinium and the guanine nitrogens are proton donors. The side chain atoms of Arg32 exhibit fairly high temperature factors in all of ... See full document
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Influenza A virus RNA-dependent RNA polymerase: analysis of RNA synthesis in vitro.
... A virus RNA-dependent RNA polymerase, purified from virion ribonucleoprotein particles and from which endogenous genomic RNA (vRNA) has been depleted by treatment with ... See full document
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