[PDF] Top 20 Interaction of Cryptococcus neoformans Extracellular Vesicles with the Cell Wall

... C. neoformans and other fungal species, we found a large functional distribution of identified hits ...these extracellular vesicles can act as “virulence bags” to deliver a high payload concentration ... See full document

... Reduced intracellular cAMP in CWI kinase deletion strains. Recent work has demonstrated the importance of the RIM101 transcription factor in the cAMP/PKA-dependent regulation of capsule production, defining a novel ... See full document

... mutant cell wall appear to be due mostly to excess chitin oligom- ...C. neoformans has demonstrated that this molecule is necessary for capsule attachment to the cell (39, ...mutant ... See full document

... Cryptococcus neoformans is an opportunistic fungal pathogen that causes cryptococcal meningoencephalitis, particularly in immunocompromised ...fungal cell wall is an excellent target for ... See full document

... Abstract: Macrophage Migration Inhibitory Factors (MIF) are pivotal cytokines/chemokines for vertebrate immune systems. MIFs are typically soluble single-domain proteins that are conserved across plant, fungal, protist, ... See full document

... To directly examine the GXM that was shed from pbx mutant cells, we compared the polysaccharide-containing CM from each single mutant to that from the wild type by resolving the material on agarose gels and ... See full document

... C. neoformans (Banks et ...in cell wall chitin are induced upon exposure of these fungi to caspofungin (Walker et ...the interaction of caspofungin treatment and a re- sponse involving chitin ... See full document

... purified vesicles, we attempted to probe for lipophilic structures in the capsule of ...C. neoformans using ...C. neoformans when stained with antibodies to 3-OH ...the cell wall in ... See full document

... in extracellular fractions might interfere with pro- tein digestion, as previously suggested for Aspergillus oryzae ...of vesicles from the highly encapsulated strain 24067 (data not ...in vesicles ... See full document

... C. neoformans wild type (H99) and CAP ...(cell wall staining), monoclonal antibodies 18B7 and 13F1, and polyclonal antisera anti-H99 and anti-GalXM, as described in Materials and ... See full document

... the extracellular space for capsule ...and cell wall clusters provides strong support for the suggestion that the capsular polysaccharide is synthesized in the cytoplasm and exported to the exterior ... See full document

... C. neoformans’ cell surface and secreted proteins in CNS invasion (17, ...of extracellular phospholipase B (29, 35), urease (11, 12), and laccase (34) con- tributes to the ability of ...C. ... See full document

... Fluorescence microscopy. Cells were grown in the YPD medium for 14 to 16 h and pelleted at 4,000 rpm. The cells were then washed once with distilled water and finally resuspended in distilled water. The cells were placed ... See full document

... ABSTRACT Cryptococcus neoformans is a human opportunistic fungal pathogen causing severe disseminated meningoencephali- tis, mostly in patients with cellular immune ...C. neoformans serotypes A and D ... See full document

... ABSTRACT Cryptococcus neoformans and Cryptococcus gattii are globally distributed human fungal pathogens and the leading causes of fungal ...C. neoformans can utilize myo-inositol as a sole ... See full document

... to Cryptococcus neoformans are common in AIDS ...C. neoformans multiplied within monocyte-derived macrophages (MDM) in the absence of chloroquine but were killed with the addition of ...C. ... See full document

... yeast cell growth in YPD and the host than is present during ex vivo CSF ...to cell metabolism, such as cellular biosynthetic pro- cesses (GO:0044249, P ⫽ 1e ⫺ 12), gene expression (GO:0010467, P ⫽ ...C. ... See full document

... C. neoformans Cdc50 for fungal virulence both in vitro and in ...during interaction with macro- phages, that cdc50⌬ cells are engulfed by macrophages at a higher rate than the wild type and are more ... See full document

... C. neoformans (Drop Cn) cells in the lungs of outbred OF1 mice were dead yeast ...thick cell wall, and one well-defined round refringent vesicle) are ...DropC. neoformans cells were devoid of ... See full document

... Microbes have developed fascinating genetic plasticity to adapt to a variety of stress conditions within a very short time through mechanisms of microevolution. Under- standing these processes and the magnitude of their ... See full document