[PDF] Top 20 Interaction between Herpes Simplex Virus Type 1 IE63 Protein and Cellular Protein p32

... The herpes simplex virus type 1 (HSV-1) immediate-early gene IE63 (ICP27), the only HSV-1 regulatory gene with a homologue in every mammalian and avian herpesvirus ... See full document

... Viral transcripts are exported less efficiently during WRL-A infection. To analyze the export of HSV-1 transcripts during WRL-A infection compared to KOS infection, nuclear and cyto- plasmic fractions of WRL-A- ... See full document

... of IE63, a transcript rescue mechanism (8), anal- ogous to that proposed for human immunodeficiency virus Rev (42), which blocks splicing via the SAP145 interaction to pre- vent nonproductive ... See full document

... Incubation of infected cell extracts with the lower-affinity site II DNA as a probe resulted in the appearance of two protein-DNA complexes with mobilities identical to those of the A an[r] ... See full document

... BiFC. BiFC analysis was performed in transient transfection assays as described previously (27). RSF were either transfected with N-Venus/ ICP27/C-Venus plasmid DNA or were cotransfected with N-Venus–TAP/ NXF1 and ... See full document

... Fluorescence microscopy of the VP22 homologs fused to GFP transfected into cell lines revealed fascinating contrasts between BVP22 and HVP22. Although both homologs could associate with host microtubules, ... See full document

... the protein synthesis machinery (17). The HSV-1 pro- tein ...the cellular phosphatase PP1 ␣ to me- diate the dephosphorylation of eIF2␣ and thus antagonizes the PKR signaling pathway ...(Fig. ... See full document

... Quantitative analysis of the interaction between the DNA polymerase subunits and HP96 by the gel mobility shift assay showed that in addition to altering the details of the nuclease prot[r] ... See full document

... of protein on the plasma membrane, suggesting that the acidic cluster may play some role in endo- cytosis ...(or cellular proteins) on the plasma membrane and directs them to the proper site of ... See full document

... mutant virus infections ...it cellular or viral, that is involved normally in the ICP0 translocation pathway from ND10s to the ...mutant virus exhibits an ICP0 phenotype similar to that of a point ... See full document

... relationship between UL15 and the large subunit of bacteriophage T4 terminase, gp17, including the presence of Walker A and B box motifs characteristic of ATP binding proteins ...homologous protein of human ... See full document

... for 1 h at ...for 1 h with 10-nm-colloidal-gold-conjugated secondary anti-mouse IgG antibody (Sigma), washed with PBS, and postfixed in a 2% glutaraldehyde-PBS ...with 1% alkaline lead citrate and 2% ... See full document

... here between the patterns of tegument-capsid interaction seen in the presence or absence of VP26 clearly eliminates the possibility that accessibility of the binding site is controlling ... See full document

... of virus on Vero ...F39H virus-infected cells icosahedral capsid structures that were evident for wild-type-infected cells ...mutant virus or in cells with mutations in the VP5 N terminus (11, ... See full document

... essential herpes simplex virus type 1 (HSV-1) immediate-early protein IE63 (ICP27) is pleiotropic in function, promoting the switch from the early to late phase of ... See full document

... Recombinant virus v-NC-Venus-ICP27 is defective in viral ...v-NC-Venus-ICP27 virus was constructed by the marker transfer of N-Venus/ICP27/C-Venus into 27-LacZ (40), replacing the 27-LacZ ...on ... See full document

... Critical for an understanding of the mechanism of HSV-1 DNA replication, and of the potential role of OBPC in this process, is a consideration of the structure of replicative inter- mediates. Recent studies using ... See full document

... The herpes simplex virus type 1 (HSV-1) regulatory protein ICP27 is a 63-kDa phosphoprotein required for viral ...that cellular kinases phosphorylate ICP27 during ... See full document

... binding protein activates ...distance. Cellular DNA binding proteins, such as high-mobil- ity-group proteins, TATA box binding proteins, and initiator binding proteins, may be involved in ICP4-mediated gene ... See full document

... virion protein must have a mecha- nism for localizing to membrane vesicles—either an intrinsic sorting signal or an interaction with a protein that possesses such a ...tegument protein, which ... See full document