[PDF] Top 20 Isolation and characterization of Caulobacter crecentus bacteriophage phi Cd1.

... 14, containing 0.2% peptone Difco, 0.1% yeast The ammonium sulfate precipitate was collected by extract, and 0.2% MgSO4 .7H2O in deionized water centrifugation at 17,500 rpm for 20 min i[r] ... See full document

... This analysis is somewhat complicated by the fact that recombinant genotypes produced during one infectious cycle can express their phenotype during that cycle of growth (Séchaud et al. 1965). However, the contribution ... See full document

... To identify the nature of the trichloroacetic acidsoluble, intracellular radioactive material, cells infected with the A2-dmp mutant were sedimented by centrifugation at various times af[r] ... See full document

... Since these mutations cause production of long fragments of the gp3l molecule in slu host cells 6, it was of interest to determine whether the byp31-1 allele would permit capsid assembly[r] ... See full document

... The percentage guanine plus cytosine compositions of the deoxyribonucleic acid, determined by melting temperature and cesium chloride equilibrium centrifugation, were 40.7 and 42.0, resp[r] ... See full document

... coli K-12 cells that are able to incorporate galactose into their cell walls, but mutants blocked in the synthesis of uridine diphosphogalactose, the precursor of cell wall galactose, ar[r] ... See full document

... According to the proposed adsorption mechanism, the following INCO substructures could harbor the bactericidal activity and inactivate cells upon their contact with the bacterial surface[r] ... See full document

... In this context, it is interesting that the phage is quite thermostable in water of the hot spring from which the phage was isolated, because the factors contained in this water is proba[r] ... See full document

... Analysis of three-factor transduction crosses involving the xtl marker with donor 44A 0, and may be analogous to the Aind- mutations xtl-1, recipient GB64, and selected phenotype argC+ a[r] ... See full document

... Although no discrepancies in the guanine-plus-cytosine content have been found for Cp-1 DNA, as determined by the melting temperature and by the buoyant density analysis see above, the r[r] ... See full document

... T h e suppression of gene 49 defects was used as a selective device, and conditional (amber and temperature-sensitive) alleles of genes uvsX and uvsY were isolate[r] ... See full document

... The molecular weight of RS1 DNA was determined employing two methods: i from the sedimentation coefficient derived from sucrose gradient centrifugation, as described by Freifelder 6, by [r] ... See full document

... Using standard overlay methods, tryptone top agar, and either tryptone or LB agar plates, R4-1 forms turbid plaques on a lawn of the sensitive host strain 014 temperatures from 30 to 40 [r] ... See full document

... 460 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest hydrochloride containing 1% Triton X-100, and the tail-lysozyme was separated from other tail components by preparat[r] ... See full document

... mutants remains the same with increased temperature of incubation, but the plaques get smaller until a temperature is reached at which plaques can no longer be discerned. Th[r] ... See full document

... Simple electron microscope examination of of bacteriophage particle assembling is a core base plates demonstrate that they are composed protein and also because the mechanism of its of a[r] ... See full document

... We examined the level of protein synthesis of proteins P6 and P3 in 41907 sus600-infected HB lOY cells at 24°C to see whether the amount of P6 and P3 syntthesized was equivalent to the e[r] ... See full document

... Accordingly, the amber mutants were screened only for such defects by i determina- tion of the buoyant density of the DNA synthesized in the nonpermissive host and ii measurement of the [r] ... See full document

... Cells infected with susl41241 phage were pulse-labeled for 3 min with [3H]leucine at 42 min after infection, and the amounts of labeled Hd and P7 proteins in proheads were determined at [r] ... See full document

... Growth of lysogenic strains was improved by cultivation in thioglycollate medium; the growth time was extended, resulting in a greater probability of spontaneous induction of a lytic cyc[r] ... See full document