[PDF] Top 20 Rapid Localization of Gag/GagPol Complexes to Detergent-Resistant Membrane during the Assembly of Human Immunodeficiency Virus Type 1

... FIG. 1. Distribution of Gag/Gag and Gag/GagPol complexes in COS7 ...of Gag/GagPol complex by immunoprecipi- tation with ...expressing Gag alone (pSVGAG) ... See full document

... with membrane binding and may not require a specific recruitment ...Unprocessed Gag polyproteins were initially soluble in RIPA buffer but became rapidly insoluble due to multimerization and were then inac- ... See full document

... intracellular localization of these two viral assembly intermediate complexes was investigated by use of a newly developed method of subcellular ...mutant Gag. However, little of the ... See full document

... HIV-2 Gag dissociates from yeast membrane in the absence of ...the membrane-binding ability of Gag, mem- brane flotation experiments were carried out using sucrose step ...HIV-1 ... See full document

... examine Gag membrane ...wild type, only the membrane flotation assay can separate membrane-bound ma- terial from non-membrane-bound protein complexes such as the recently ... See full document

... The human immunodeficiency virus type 1 (HIV-1) Gag polyprotein contains information sufficient for the assembly and release of enveloped virions (19, ...the ... See full document

... HIV assembly process. Other viral proteins such as gp41 may target Gag proteins more specifically to lipid rafts, as suggested by a recent report ...that Gag briefly interacts with lipid rafts before ... See full document

... CA146 Gag proteins are unable to coassemble with full-length Gag into VLPs, we cannot exclude the possibility that they may retain some ability to form low- order ...(MA-CA) Gag proteins can form ... See full document

... The Gag polyprotein of human immunodeficiency virus type 1 (HIV-1) organizes the assembly of nascent virions at the plasma membrane of infected ...of ... See full document

... are resistant to detergent at low temperature (41) and are frequently described as detergent-resistant membranes (DRMs) (5, 6, ...of detergent-lysed cells in sucrose ...of ... See full document

... wt Gag-expressing cells, the surface staining pattern of Env in 8N Gag-expressing cells is strikingly different ...of Gag at the plasma membrane restricts Env localization at the sur- ... See full document

... tant Gag precursors that lacked the C-terminal p6 domain or p6 plus the p1 interdomain ...these Gag processing inter- mediates appeared selectively enriched in the particulate frac- ...only Gag ... See full document

... endogenous human ARF1 were (forward) 5’-GTGACCACCATTCCCACCATAG-3’ and (reverse) 5’-TCATTGCTGTCCACCACGAAG- 3’ as described elsewhere ...for 1 min, followed by melting curves generated from 65°C to 95°C, at ... See full document

... The assembly experiments were performed exactly as de- scribed previously (6), except that in some experiments the assembly reaction was scaled up to contain twice the amounts of all constituents in twice ... See full document

... of Gag-specific CTL responses. Because Gag- specific CTL may be especially important for control of viremia (16, 26), the low set point viremia in subject 00015 suggests a lack of deficiency in his ... See full document

... Table 1 shows the levels of CAT expression found when comparing these pHC(21)k/o and pHCF(21)k/o constructs with their parental pHC(21) and pHCF(21) ...(experiment 1), capped RNAs (experiments 2 and 3), and ... See full document

... To determine whether regions important for the association of Vif with membranes are also important for function, a transient complementation assay was used to examine the abilities of t[r] ... See full document

... of Gag leads to significant recovery of RC compared to protease ...of Gag has some impact, possibly due to the four mutations at the p2/p7 cleavage ...the Gag polyprotein can play a key role in RC ... See full document

... of Gag is mediated by its N-terminal domain, ...sarcoma virus [RSV]), MA bears no fatty acid ...in membrane in- teraction (16, 40, 43, ...direct Gag to the plasma membrane than short ... See full document

... rupt Gag-Gag ...particle assembly, suggesting that this change may disrupt CA-CA con- tacts involved in the immature virion ...abolished Gag-Gag ...the Gag- Gag ... See full document