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A loop mediated isothermal amplification assay for the visual detection of duck circovirus

A loop mediated isothermal amplification assay for the visual detection of duck circovirus

... the amplification in order to elimin- ate the chance of ...to amplification, calcein combines with the manganese ions (Mn 2+ ) and the reaction solution turns ...LAMP amplification reaction proceeds, ... See full document

6

Visual detection of H3 subtype avian influenza viruses by reverse transcription loop mediated isothermal amplification assay

Visual detection of H3 subtype avian influenza viruses by reverse transcription loop mediated isothermal amplification assay

... transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to detect the H3 subtype AIVs ...and assay reaction conditions were optimized. The established ... See full document

10

Reverse transcription, loop mediated isothermal amplification assay for the sensitive and rapid detection of H10 subtype avian influenza viruses

Reverse transcription, loop mediated isothermal amplification assay for the sensitive and rapid detection of H10 subtype avian influenza viruses

... The loop-mediated isothermal amplification (LAMP) technique was invented in 2000 [17] and provides a power- ful gene amplification tool with simple conditions requiring only a water ... See full document

7

Development of a Loop Mediated Isothermal Amplification Assay for Rapid Detection of BK Virus

Development of a Loop Mediated Isothermal Amplification Assay for Rapid Detection of BK Virus

... LAMP assay produces a large amount of amplified product, resulting in easier detection by visual inspection, by observation of either an increase in turbidity caused by gener- ation of magnesium ... See full document

7

Loop mediated isothermal amplification for the detection of goose circovirus

Loop mediated isothermal amplification for the detection of goose circovirus

... ward loop primers (FL and BL) which enable faster formation of loop and hairpin-like structures allowing faster detection of the specific ...the detection of many important poultry and ... See full document

11

Rapid and Simple Method for Detecting the Toxin B Gene of Clostridium difficile in Stool Specimens by Loop Mediated Isothermal Amplification

Rapid and Simple Method for Detecting the Toxin B Gene of Clostridium difficile in Stool Specimens by Loop Mediated Isothermal Amplification

... immunological detection of TcdA alone or of both TcdA and ...culture assay (6, 19, 22), and the infection caused by TcdA-negative, TcdB-positive (A ⫺ B ⫹ ) ...TcdA detection kit (1, 3, 16, 17, ...PCR ... See full document

5

Loop Mediated Isothermal Amplification Assay for Rapid Detection of Common Strains of Escherichia coli

Loop Mediated Isothermal Amplification Assay for Rapid Detection of Common Strains of Escherichia coli

... for detection of UTI-associated ...the assay in its current format. This assay is suited for qualitative but not quantitative ...this assay is more likely to be an adjunct rather than a ... See full document

5

Loop Mediated Isothermal Amplification Method for Rapid Detection of the Periodontopathic Bacteria Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola

Loop Mediated Isothermal Amplification Method for Rapid Detection of the Periodontopathic Bacteria Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola

... the detection of these organ- isms provides essential information on the severity of peri- ...rapid detection of these three ...the detection of oral bacteria because over 500 species or phylotypes ... See full document

7

Rapid Extraction Method of Mycobacterium ulcerans DNA from Clinical Samples of Suspected Buruli Ulcer Patients

Rapid Extraction Method of Mycobacterium ulcerans DNA from Clinical Samples of Suspected Buruli Ulcer Patients

... Abstract: Isothermal amplification techniques such as recombinase polymerase amplification (RPA) and loop-mediated isothermal amplification (LAMP) for diagnosing Buruli ... See full document

11

Development of a rapid loop mediated isothermal amplification assay for diagnosis and assessment of cure of Leishmania infection

Development of a rapid loop mediated isothermal amplification assay for diagnosis and assessment of cure of Leishmania infection

... molecular assay to detect parasite DNA in clin- ical samples, therefore, we compared the threshold detec- tion limit of kDNA copies by LAMP ...Q-PCR assay. The threshold copy number detection limits ... See full document

9

<p>Loop-mediated isothermal amplification for detection of <em>Legionella pneumophila</em> in respiratory specimens of hospitalized patients in Ahvaz, southwest Iran</p>

<p>Loop-mediated isothermal amplification for detection of <em>Legionella pneumophila</em> in respiratory specimens of hospitalized patients in Ahvaz, southwest Iran</p>

... L. pneumophila strains were detected in seven sputum samples (7%) using LAMP (Figure 2). Also, samples that were positive on culture and PCR showed positive results on LAMP assay. L. pneumophila was not found in ... See full document

6

Loop Mediated Isothermal Amplification for Rapid Detection of Newcastle Disease Virus

Loop Mediated Isothermal Amplification for Rapid Detection of Newcastle Disease Virus

... the loop-mediated isothermal amplification (LAMP) assay for the rapid, simple, and sensitive detection of Newcastle disease virus (NDV) directly from culture isolates as well as ... See full document

5

Loop Mediated Isothermal Amplification (LAMP) Assay for GMO on: Recent Progresses and Future Perspectives

Loop Mediated Isothermal Amplification (LAMP) Assay for GMO on: Recent Progresses and Future Perspectives

... GMO detection method is perfect, neither is ...GMO detection methods; however, obvious shortages of it also emerge along the analysis ...non-specificity amplification from specificity ... See full document

9

Detection of helminths by loop-mediated isothermal amplification assay: a review of updated technology and future outlook

Detection of helminths by loop-mediated isothermal amplification assay: a review of updated technology and future outlook

... Loa loa is a long-neglected filariae that is reported to cause deadly serious adverse events after ivermectin treatment [86, 89–91, 175, 176] at a low threshold of microfilaria (mf ) burden [175], where determination of ... See full document

22

Development of a Loop Mediated Isothermal Amplification Assay for Rapid Detection of Subgroup J Avian Leukosis Virus

Development of a Loop Mediated Isothermal Amplification Assay for Rapid Detection of Subgroup J Avian Leukosis Virus

... the amplification of the EAV-HP ...the amplification tem- perature, and the reaction time (data not shown), in which the recombinant plasmid containing the 545-bp NX0101 gene frag- ment was used as the ... See full document

6

Loop Mediated Isothermal Amplification Assay for Detection of Haemophilus influenzae Type b in Cerebrospinal Fluid

Loop Mediated Isothermal Amplification Assay for Detection of Haemophilus influenzae Type b in Cerebrospinal Fluid

... LAMP assay used here was more analytically sensitive and specific than slide agglutination ...lower detection limit of the Hib LAMP assay is in agreement with our previously reported findings for ... See full document

7

Detection of Four Plasmodium Species by Genus  and Species Specific Loop Mediated Isothermal Amplification for Clinical Diagnosis

Detection of Four Plasmodium Species by Genus and Species Specific Loop Mediated Isothermal Amplification for Clinical Diagnosis

... perfect assay for quantifi- ...detectable amplification caused by the lower template copy number makes the enzyme activity lower and (ii) the lower template DNA concentration, which decreases the annealing ... See full document

8

Sensitive and Rapid Detection of the New Delhi Metallo Beta Lactamase Gene by Loop Mediated Isothermal Amplification

Sensitive and Rapid Detection of the New Delhi Metallo Beta Lactamase Gene by Loop Mediated Isothermal Amplification

... LAMP assay (19) (LAMP amplifies DNA with high efficiency under isothermal con- ditions, LAMP is highly specific for the target sequence, and LAMP is simple and easy to perform), it shows a high rate of ... See full document

6

Loop Mediated Isothermal Amplification for Detection of African Trypanosomes

Loop Mediated Isothermal Amplification for Detection of African Trypanosomes

... Loop-mediated isothermal amplification of DNA (LAMP) may provide one ...under isothermal conditions. Since the LAMP reaction is done under isothermal conditions (63 to 65°C), ... See full document

8

&lt;p&gt;Multiplex loop-mediated isothermal amplification (multi-LAMP) assay for rapid detection of &lt;em&gt;mcr-1&lt;/em&gt; to &lt;em&gt;mcr-5&lt;/em&gt; in colistin-resistant bacteria&lt;/p&gt;

<p>Multiplex loop-mediated isothermal amplification (multi-LAMP) assay for rapid detection of <em>mcr-1</em> to <em>mcr-5</em> in colistin-resistant bacteria</p>

... chromosomally mediated; however, the discovery of plasmid-mediated colistin resistance via mcr-1 in 2015 revealed colistin resistance was capable of horizontal ... See full document

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