[PDF] Top 20 Mapping chromatin modifications at the single cell level
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Mapping chromatin modifications at the single cell level
... amplification, cell- specific barcodes are ...modification. Single cells are sorted into tubes and calcium is added to trigger MNase-mediated ...enables cell-specific barcode incorporation. (F) ... See full document
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Chromatin accessibility dynamics in the Arabidopsis root epidermis and endodermis during cold acclimation
... A second issue holding DNase-seq back is the use of agarose gels and agarose gel plugs. To prevent mechanical shearing of DNA, agarose gel plugs are required in DNase-seq protocols (Boyle et al. (2008a); Crawford et al. ... See full document
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Polyomavirus Small T Antigen Controls Viral Chromatin Modifications through Effects on Kinetics of Virus Growth and Cell Cycle Progression
... NG59 shows higher levels of phosphorylation of histone H3 at serine 10 than does WT virus. PP2A is the only known cellular target of sT, suggesting that histone phosphorylation may be affected by the presence or absence ... See full document
8
Chromatin reprogramming during the somatic to reproductive cell fate transition in plants
... H3 modifications were calculated as a ratio of the intensity sum in the antibody channel over that in the DNA staining ...the chromatin volume was derived by subtracting the nucleolus volume (devoid of DNA ... See full document
12
Suppression of Viral Gene Expression in Bovine Leukemia Virus-Associated B-Cell Malignancy: Interplay of Epigenetic Modifications Leading to Chromatin with a Repressive Histone Code
... Our findings suggesting a correlation of DNA methylation with the silent phenotype in the transformed L267 B cells are in contrast with those of earlier studies indicating the absence of or minimal CpG methylation of the ... See full document
11
Lysine Post-Translational Modifications of Saccharomyces Cerevisiae Chromatin Proteins
... PTM studies generally involve observing when and where in the cell (and along the genome for a chromatin PTM) it is present. While mass spectrometry can be used in some cases, antibodies specific to the ... See full document
217
Thyroid hormone receptor actions on transcription in amphibia: The roles of histone modification and chromatin disruption
... In addition to histone acetylation, histone methylation and phosphorylation have also been implicated to play a role in gene regulation by TR in the frog oocyte transcription system [75]. During Xenopus tropicalis ... See full document
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Altered Chromatin Occupancy of Master Regulators Underlies Evolutionary Divergence in the Transcriptional Landscape of Erythroid Differentiation
... erythroid cell lines suggesting that species-specific differences in transcription may be due to evolu- tionary divergence in TF occupancy and the epigenome ...of chromatin structure and TF occupancy ... See full document
20
Chromatin organization changes during the establishment and maintenance of the postmitotic state
... specific chromatin modifications asso- ciated with entry into or exit from ...during cell cycle exit rather than obvious changes in total levels, we do observe a strong upregulation of H4K16 ... See full document
20
Waves of chromatin modifications in mouse dendritic cells in response to LPS stimulation
... same cell type and stimulus showed that most changes in gene expression patterns were controlled at the transcriptional level, without widespread changes in RNA degradation rates [25, ...histone ... See full document
15
Molecular genetic diagnosis of single gene disorders at the single cell level
... CVS was first performed transcervically (Mohr, 1968), but was unpopular due to the more acceptable amniocentesis. The development of modem high resolution ultrasound has helped CVS in ensuring its safety and it is ... See full document
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Distinctive patterns of epigenetic marks are associated with promoter regions of mouse LINE-1 and LTR retrotransposons
... H2A.Z chromatin-bound frag- ments was purified and subjected to the second ChIP with H3K4me2, H3K9me3, AcH4K16, H4K20me3 and HP1α antibodies to determine possible co-occupancy with H2A and ... See full document
7
The genetics of neuroendocrine prostate cancers: a review of current and emerging candidates
... Abstract: Prostate cancer (PC) displays a strong familial link and genetic factors; genes regulating inflammation may have a pivotal role in the disease. Epigenetic changes control chromosomal integrity, gene functions, ... See full document
6
Heteroresistance at the Single Cell Level: Adapting to Antibiotic Stress through a Population Based Strategy and Growth Controlled Interphenotypic Coordination
... how cell populations control CTX-M-14 gene expression, adapt to different antibiotic dosages, and thus display ...a cell dedicates itself to division and growth, generally speaking, gene expression other ... See full document
9
HCF1 and OCT2 Cooperate with EBNA1 To Enhance OriP-Dependent Transcription and Episome Maintenance of Latent Epstein-Barr Virus
... host cell factor 1 (HCF1), which is also involved in the transcriptional activation of host cell cycle genes (27, ...several chromatin-modify- ing complexes, including the histone H3 lysine 4 (H3K4) ... See full document
15
Decrease of 5hmC in gastric cancers is associated with TET1 silencing due to with DNA methylation and bivalent histone marks at TET1 CpG island 3′-shore
... 76 single-read ...a mapping step was performed with the methylation-specific mapping tool BRAT [47] for short bisulfite-treated reads because unmethylated cytosines within sequences read from RRBS ... See full document
16
'Memory and molecular turnover,’ 30 years after inception
... C are estimated at 40% of the 5Me C levels in this re- gion of the brain. In Purkinje and granule cell neurons in the brain 5hm C is estimated to be 0.6% and 0.2% of total nucleotides, respectively [36]. Tet1 ... See full document
9
Spt10 and Spt21 Are Required for Transcriptional Silencing in Saccharomyces cerevisiae
... Several results suggest that Spt10 is functionally related to another protein, Spt21. Mutations in either gene cause the unusual phenotype of permitting transcription to initiate from the 3 ⬘ long terminal repeat (LTR) ... See full document
12
Evidence for a second ankylosing spondylitis associated RUNX3 regulatory polymorphism
... different cell types highlights a role for Figure 5 rs4265380 interacts with ...monocytes chromatin states, ENCODE histone modification and p300 transcription factor chromatin immunoprecipitation ... See full document
10
The stem cell niche: tissue physiology at a single cell level
... The niche exerting fitness pressures on the stem cell is particu- larly intriguing in hematopoiesis because of the known circulation of those stem cells in the adult. The question of why stem cells continue to ... See full document
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