[PDF] Top 20 Mapping of Functional Elements in the Stem-Anchor Region of Tick-Borne Encephalitis Virus Envelope Protein E

... truncated E proteins were secreted together with the mature M protein and that the kinetics of protein E secretion were essentially identical for all of the constructs shown in ...of ... See full document

... The E protein of TBE virus forms flat head-to-tail homo- dimers (24) that are oriented parallel to the viral membrane and organized in an icosahedral lattice (9), another character- istic that ... See full document

... flavivirus envelope (E) protein is located on the virion surface and is responsible for receptor recognition, virus entry, and subsequent virus-host membrane ...fusion. E ... See full document

... and E are encoded in adjacent regions of the flavivirus genome, which contains a single long open reading frame for all of the structural and nonstructural proteins ...viviral envelope proteins (reviewed in ... See full document

... the virus, it can, at the same time, make it more difficult for the researcher interested in studying the role of indi- vidual proteins and functional elements in ...TM2 region of the ... See full document

... of protein C, the packaging of the genome, and budding occur (almost) simultaneously and are functionally closely interrelated processes ...mature protein C from the adjacent protein ...of ... See full document

... the stem region for representative sequences of the four major flavivirus ...-helix; E, b -sheet) and the second line gives the reliability for the secondary-structure prediction, as reported by the ... See full document

... of tick-borne encephalitis (TBE) virus envelope proteins prM and E in mammalian cells leads to their incorporation into enveloped recombinant subviral particles (RSPs), which ... See full document

... native protein—is oriented perpendicular to the membrane ...the stem-anchor region was not present in the truncated soluble E trimer (sE trimer) structure, the orientation of domain III ... See full document

... The envelope protein E of flaviviruses mediates both receptor-binding and membrane ...of E are tightly packed and organized in a herringbone-like icosahedral structure, whereas in ... See full document

... N-terminal protein C-coding region of TBEV is nones- ...the region coding for the N-terminal part of protein C is essential for replication of mosquito-borne flaviviruses (1, 2, 10, 27, ... See full document

... The envelope protein E is a class II viral fusion ...other envelope protein, prM, is cleaved by furin during viral maturation and the pr moiety is released as a result of conformational ... See full document

... capsid protein of the flavivirus tick-borne encephalitis (TBE) ...capsid protein sequences and part of which may form an amphipathic ...affect protein expression and up to a ... See full document

... the E protein remained Triton X-100 soluble ...of E dimers shown in ...the E pro- tein is more resistant to heating than the dimeric ...the E dimer at temperatures below the aggregation ... See full document

... with tick-borne encephalitis (TBE), early cns neuroborreliosis (NB), and other aseptic meningitis (AM) on admission to hospital (I), 10 – 14 days later (II) and 4 – 6 weeks later (III), as well as in ... See full document

... for tick-borne encephalitis virus (TBEV) in Mongolia, to isolate TBEV by in vivo methods and determine subtypes of ...and virus neutralization (VN) ... See full document

... of E proteins in which the dimer interface and lateral ridges of all three domains support a stable icosahedral framework (31, ...41). E protein homodimeriza- tion has been thought to be a primary ... See full document

... of tick-borne encephalitis (TBE) virus can be divided into a variable region that is highly heterogeneous in length among strains of TBE virus and in certain cases includes an ... See full document

... the E protein (compare ...any E trimer formation or other change in oligomeric state (data not ...with E and functions in a manner identical to that in ... See full document

... ick-borne encephalitis virus (TBEV) is a major human-patho- genic flavivirus that is endemic in Europe and Asia ...duced virus-neutralizing antibody titers and IgG antibody titers, which ... See full document