[PDF] Top 20 Mechanistic Studies of a T20-Dependent Human Immunodeficiency Virus Type 1 Variant

... inhibitors T20, C34, and T1249 (15), had the greatest induction ...the T20-dependent virus because an intrinsic prediction of the mechanistic model is that these reagents should not ... See full document

... by human immunodeficiency virus (HIV) typically requires an interaction between the viral envelope glycoprotein (Env), CD4, and a chemokine receptor, CD4-independent isolates of HIV and simian ... See full document

... Infection of primary CD4 ⴙ and CD8 ⴙ cells. Although it is CD4 independent, the 93US143-T8 isolate is known to effi- ciently infect both CD4 ⫹ and CD8 ⫹ cells. In addition, 93US143- T8, as well as its parental 93US143 ... See full document

... The effect of a late-domain mutation on HIV-1 Gag ubiq- uitination depends on membrane binding. Our collective results indicate that the increased ubiquitination of HIV-1 carrying a mutation in the PTAP ... See full document

... E89G variant of HIV-1 RT to nucleoside analogs have come from biochemical studies of recombinant mutant enzymes (6, 15, 37) and X-ray structural information about the wild-type enzyme (6, 13, ... See full document

... recent studies have indicated the involvement of host cell factors in human immunodeficiency virus type 1 (HIV-1) ...quantified virus-like particle (VLP) production ... See full document

... of human immunodeficiency virus type 1 ...a virus binding enzyme-linked immunosorbent assay. Virus progeny raised in peripheral blood mononuclear cells expressed most of ... See full document

... HIV-1 Vif is able to bind APO3G C97A. Previous data showed that Vif-induced degradation of APO3G requires the physical interaction of the two proteins. Indeed, a single amino acid change in APO3G at residue 128 ... See full document

... two virus variants, each with a distinct change, A92E or G94D, in the CypA-binding loop of CA (1, ...Some studies have suggested that the level of CypA expression in the target cell may affect the ... See full document

... In eukaryotic cells, 10 cdk’s have thus far been identified. Aside from the function of regulating cell cycle progression, they are involved in a broad range of biological processes, such as transcription, DNA repair, ... See full document

... by studies that failed to observe a role for p6 in virus release (22, 23, 27, 32, 33, 46, 49, ...in virus release (24) suggests that PR may also be a factor in differences among studies, since ... See full document

... the virus-encoded protease, NC coats viral genomic RNA and subsequently influences early events in the viral life cycle such as reverse transcription and possibly even integration (3, 4, 8, ...structural ... See full document

... substitutions were introduced into pN36/34 by the method of Kunkel et al. (21) and then verified by DNA sequencing. All recombinant peptides were expressed in Escherichia coli strain BL21(DE3)/pLysS (Novagen, Madison, ... See full document

... different studies analyzing wild-type proteins (27, 40, 52, 53) and protein or virus mutants (4, ...Crm1- dependent export pathway by accumulation of an internal control protein inside the ... See full document

... support virus binding could be directly compared to that of wt ...peat variant reached 56% of the level for the parental con- struct ...functional studies of the DC-SIGN variants were carried out ... See full document

... the virus, the middle panel shows a side view with the virus membrane positioned above and the target cell below, and the bottom panel shows a view from the perspective of the target ... See full document

... Fig. 1 that express either the CCR5 or CXCR4 coreceptor were inoculated with serial dilutions of NL4-3-derived viruses bearing R5 (A), X4 (B), or dually tropic (C) envelope ...of virus-containing ... See full document

... In vitro replication of the reporter constructs. To investigate the kinetics of in vitro replication and HSA expression of NL-r-HSAS and NL-r-HSAL, PHA-stimulated PBMC were infected with 170 ng of p24 Gag of either ... See full document

... Reversibility of the CypA-imposed block to integration by CsA-dependent mutants in arrested cells. Our results with the A92E/P90A double mutant indicated that CypA-CA interac- tions inhibit infection by the A92E ... See full document

... Under the selective pressure of protease inhibitor therapy, patients infected with human immunodeficiency virus (HIV) often develop drug-resistant HIV strains. One of the first drug-resistant ... See full document