[PDF] Top 20 Loop-mediated isothermal amplification (LAMP) assays for the species-specific detection of Eimeria that infect chickens
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Loop-mediated isothermal amplification (LAMP) assays for the species-specific detection of Eimeria that infect chickens
... infecting species is required [9]. Detection of sub- clinical infection can be particularly demanding in the absence of ...random amplification of polymorphic DNA PCR (RAPD-PCR) and DNA ... See full document
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Loop-mediated Isothermal Amplification (LAMP) Assays for the Speciesspecific Detection of Eimeria that Infect Chickens
... Eimeria species parasites, protozoa which cause the enteric disease coccidiosis, pose a serious threat to the production and welfare of ...control Eimeria is common and sub-clinical infection is ... See full document
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Loop Mediated Isothermal Amplification for Rapid Detection of Newcastle Disease Virus
... both LAMP and nested PCR assays requires knowledge of the gene sequences, careful primer de- sign, and assay ...Designing LAMP primers is cru- cial in optimizing the LAMP reaction because ... See full document
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Development and evaluation of a Loop Mediated Isothermal Amplification (LAMP) technique for the detection of hookworm (Necator americanus) infection in fecal samples
... Hookworm has a direct lifecycle. The infective stage is a third stage larva that penetrates the skin upon contact with contaminated soil. While in the soil the third stage larvae are in a state of developmental arrest; ... See full document
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Wolbachia detection in insects through LAMP: loop mediated isothermal amplification
... identified in the ovaries of the mosquito Culex pipiens [7], and recent studies have estimated that 40% of terrestrial arthropod species are infected with the bacterium [8]. Critically, infections do not occur in ... See full document
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Specific, sensitive and rapid detection of human plasmodium knowlesi infection by loop mediated isothermal amplification (LAMP) in blood samples
... of LAMP to amplify DNA under isothermal conditions within an hour sug- gests that LAMP could be a potential alternative for detection of ...using LAMP assay is due to its ... See full document
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Detection of helminths by loop-mediated isothermal amplification assay: a review of updated technology and future outlook
... than detection using specimens from humans [8, 159, 168, 169, ...167–169]. LAMP can also act as an excellent surrogate for PCR in this ...volvulus detection targeting OvGST1a, without crossreactivity ... See full document
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Subpatent malaria in a low transmission African setting: a cross sectional study using rapid diagnostic testing (RDT) and loop mediated isothermal amplification (LAMP) from Zambezi region, Namibia
... the detection of asympto- matic ...by LAMP, and after excluding LAMP negatives (including 15 of the 16 RDT positives), sen- sitivity of RDT using LAMP as gold standard was ... See full document
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Operational Feasibility of Using Loop Mediated Isothermal Amplification for Diagnosis of Pulmonary Tuberculosis in Microscopy Centers of Developing Countries
... of LAMP for pulmo- nary TB in smear-positive, culture-positive specimens was ...of LAMP was ...of LAMP in the 220 culture- positive specimens was ...of LAMP for the detection of ...by ... See full document
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Real Time Reverse Transcription Loop Mediated Isothermal Amplification for Rapid Detection of Rift Valley Fever Virus
... Sensitivity and specificity. The L segment of the RVFV genome was used to design primers, as it encodes the viral polymerase and was thus more prone to contain conserved sequences than the S and M segments. We initially ... See full document
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Loop mediated isothermal amplification applied to filarial parasites detection in the mosquito vectors: Dirofilaria immitis as a study model
... and specific. The cost of LAMP reaction is comparative to PCR reaction, and it can be carried out with lower cost due to no requirement for a thermal cycler but only warm ...that LAMP can be well ... See full document
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Development of loop mediated isothermal amplification (LAMP) for simple detection of Leishmania infection
... Also, LAMP is particularly prone to contamination due to the large amount of DNA that it can generate, and its capability to amplify minute amounts of DNA. During this study, we nevertheless encountered multiple ... See full document
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Evaluation of Reverse Transcription Loop Mediated Isothermal Amplification assays for Rapid Detection of Human Enterovirus 71 and Coxsackievirus A16 in Clinical Samples
... CVA16 detection was slightly less sensitive than ...RT-LAMP assays for EV71 and CVA16 was 100% and ...the detection rate of EV71 and CVA 16 using RT-LAMP varied depending on the origin ... See full document
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Development of a Loop Mediated Isothermal Amplification Assay for Rapid Detection of Subgroup J Avian Leukosis Virus
... a loop-mediated isothermal amplification (LAMP) assay for rapid detection of ALV-J from culture isolates and clinical ...ALV-J-specific LAMP assay efficiently ... See full document
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Detection of Four Plasmodium Species by Genus and Species Specific Loop Mediated Isothermal Amplification for Clinical Diagnosis
... and LAMP was prepared as described previously ...of LAMP targets and priming sites for Plasmodium genus (A) and four Plasmodium species ...the species-specific primer annealing ... See full document
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Loop Mediated Isothermal Amplification Method for Rapid Detection of the Periodontopathic Bacteria Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola
... the detection of these organ- isms provides essential information on the severity of peri- ...the LAMP method for the rapid detection of these three ...the LAMP assays for these ... See full document
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Rapid Detection of Candida auris Based on Loop Mediated Isothermal Amplification (LAMP)
... Traditional methods have proven to be unsuitable for accurate identification of C. auris. Automated identification systems popularly used in clinical laboratories, like the Vitek 2 YST card (bioMérieux, Marcy I’Etoile, ... See full document
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Rapid and Simple Method for Detecting the Toxin B Gene of Clostridium difficile in Stool Specimens by Loop Mediated Isothermal Amplification
... by LAMP. DNA extraction from cultured isolates for a LAMP assay was performed in the same manner as previously described for PCRs of the toxin genes ...instructions. LAMP was also applied to ... See full document
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Rapid Detection of the Marek's Disease Viral Genome in Chicken Feathers by Loop Mediated Isothermal Amplification
... the LAMP method described here will not differentiate CVI988 strain and field MD virus/viru- lent ...the LAMP assay is most likely to be one of the field ... See full document
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Loop mediated isothermal amplification (LAMP) for point of care detection of asymptomatic low density malaria parasite carriers in Zanzibar
... The LAMP assay was performed using Loopamp™ MALARIA Pan Detection Kit (Eiken Chemical Company, Japan) [15]. The Loopamp kit has been described in detail previously [13]. Briefly, 30 μl of diluted DNA ... See full document
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