[PDF] Top 20 Monoclonal antibody capture enzyme linked immunosorbent assay for detection of bovine enteric coronavirus
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Monoclonal antibody capture enzyme linked immunosorbent assay for detection of bovine enteric coronavirus
... Plates 96-well were sensitized with an optimal dilution of monoclonal antibodies E7, G9, and H10 previously determined by titration by each of the three binding methods described above a[r] ... See full document
6
Detection of Cryptosporidium parvum oocysts in bovine feces by monoclonal antibody capture enzyme linked immunosorbent assay
... The capture ELISA was suitable for diagnostic analysis of bovine fecal samples and for assessment of oocyst shedding in experimentally infected calves.. This assay may also prove useful [r] ... See full document
5
Serological diagnosis of bovine neosporosis by Neospora caninum monoclonal antibody based competitive inhibition enzyme linked immunosorbent assay
... in bovine, caprine, and ovine abortions has been demonstrated experi- mentally (7, 20, 23, ...of bovine abor- tions in various regions of the world indicate that Neospora infections account for 3 to 42% of ... See full document
6
Detection of H5 Avian Influenza Viruses by Antigen-Capture Enzyme-Linked Immunosorbent Assay Using H5-Specific Monoclonal Antibody
... antigen detection methods are deficient in their abilities to differentiate subtypes of AIVs, which might cause unnecessary concerns over non-H5 AIV ...antigen detection methods, such as immunofluorescence ... See full document
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Detection of Ebola Viral Antigen by Enzyme Linked Immunosorbent Assay Using a Novel Monoclonal Antibody to Nucleoprotein
... Epitope mapping of capture MAb 3-3D. Since only authentic Reston subtype NP and rNP of subtype Zaire were available to us, it was unclear if this antigen-capture system works with NPs of other subtypes of ... See full document
5
Serotype 1 Specific Monoclonal Antibody Based Antigen Capture Immunoassay for Detection of Circulating Nonstructural Protein NS1: Implications for Early Diagnosis and Serotyping of Dengue Virus Infections
... antigen capture enzyme-linked immunosorbent assay (ELISA) for early detection and serotyping of DV serotype 1 (DV1) by using well-characterized monoclonal antibodies ... See full document
7
Monoclonal antibody based sandwich enzyme linked immunosorbent assay for detection of Bordetella pertussis filamentous hemagglutinin
... Monoclonal antibody 16-2-D9 and biotinylated monoclonal antibody 15-2-A5 A and polyclonal goat anti-FHA serum and monoclonal antibody 16-1-E3 B were used as capture and second antibodies[r] ... See full document
6
Monoclonal antibodies to surface antigens of Mycobacterium tuberculosis and their use in a modified enzyme linked immunosorbent spot assay for detection of mycobacteria
... spot assay. The capture spot ELISA was adapted from the assay of Spira and Scharff (15) for the purpose of capturing and visualizing a single ...first capture antibody by incubating a ... See full document
8
Detection of Anti Arboviral Immunoglobulin G by Using a Monoclonal Antibody Based Capture Enzyme Linked Immunosorbent Assay
... Monoclonal antibody (MAb)-based capture enzyme-linked immunosorbent assays (ELISAs) for the detec- tion of anti-arboviral immunoglobulin G (IgG ELISAs) were developed for a ... See full document
5
Validation of a Monoclonal Antibody-Based Capture Enzyme-Linked Immunosorbent Assay for Detection of Campylobacter fetus
... There was a 100% correlation between the culture and the ELISA results for samples tested in Canada, but there were seven culture-negative and ELISA-positive samples from England which affected the overall specificity. ... See full document
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Simple diagnosis of Encephalitozoon sp microsporidial infections by using a panspecific antiexospore monoclonal antibody
... sp. monoclonal anti- ...One antibody-se- creting hybridoma was selected from over 1,000 hybridomas, as it was the only one that met the criterion of recognition of all Encephalitozoon species which cause ... See full document
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Development of an Antigen Capture Immunoassay Based on Monoclonal Antibodies Specific for Dengue Virus Serotype 2 Nonstructural Protein 1 for Early and Rapid Identification of Dengue Virus Serotype 2 Infections
... Preparation of recombinant NS1 protein. The gene encoding the NS1 protein was amplified from total viral RNA extracted from DENV2-infected C6/36 cells by RT-PCR with a forward primer (5 ⬘ -CGGGATCCGATAGTGGTTGCGTT ... See full document
8
Identification of pseudorabies virus exposed swine with a gI glycoprotein enzyme linked immunosorbent assay
... A monoclonal antibody specific for the gI glycoprotein of virulent pseudorabies virus was produced and used gI was used in an enzyme-linked immunosorbent assay ELISA that identified and [r] ... See full document
6
Detection of Francisella tularensis in Biological Specimens Using a Capture Enzyme-Linked Immunosorbent Assay, an Immunochromatographic Handheld Assay, and a PCR
... early detection of Francisella tularensis, the causative agent of tularemia, is important for adequate treatment by antibiotics and the outcome of the ...new capture enzyme-linked ... See full document
5
Detection of hepatitis A virus and antibody by solid phase radioimmunoassay and enzyme linked immunosorbent assay with monoclonal antibodies
... Of the 25 fecal specimens collected from the group of patients with acute hepatitis A infection, HAV was detected in the same 9 specimens by all the tests being evaluated SPRIA with huma[r] ... See full document
6
Humoral immune response against lipopolysaccharide and cytoplasmic proteins of Brucella abortus in cattle vaccinated with B. abortus S19 or experimentally infected with Yersinia enterocolitica serotype 0:9.
... Several authors have proposed the use of antigenic compo- nents different from LPS as a means to improve the diagnosis of bovine brucellosis (4, 8, 12). As far as we know, however, only two studies (3, 26) have ... See full document
5
Diagnostic efficacy of monoclonal antibody based sandwich enzyme linked immunosorbent assay (ELISA) for detection of Fasciola gigantica excretory/secretory antigens in both serum and stool
... Livers of infected cattle were obtained from a local abat- toir at Giza District, Egypt. Live intact F. gigantica adult worms were collected from the bile ducts and thor- oughly washed at room temperature with 0.9% ... See full document
7
Monoclonal Antibody Based Competitive Enzyme Linked Immunosorbent Assay for Detection of Morbillivirus Antibody in Marine Mammal Sera
... Comparison of each cELISA with VNT for detection of CDV-PDV antibody yielded relative sensitivities and specific- ities of 97.6% or greater. It was observed that sample quality had an adverse effect on test ... See full document
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Monoclonal Antibodies Specific for Immunorecessive Epitopes of Glucuronoxylomannan, the Major Capsular Polysaccharide of Cryptococcus neoformans, Reduce Serotype Bias in an Immunoassay for Cryptococcal Antigen
... for detection of glucuronoxylomannan (GXM), the major capsular polysaccharide of Crypto- coccus neoformans, is an important tool for diagnosis of ...on detection with polyclonal antibodies may show serotype ... See full document
5
Comparison of enzyme linked immunosorbent assay with enzyme linked fluorescence assay with automated readers for detection of rubella virus antibody and herpes simplex virus
... The enzyme-linked immunosorbent assay ELISA was compared with the enzyme-linked fluorescence assay ELFA for the detection of rubella antibody and herpes simplex virus antigen.. Test para[r] ... See full document
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